Factors related to occurrence and distribution of selected bacterial and protozoan pathogens in Pennsylvania streams

Duris, Joseph W.; Reif, Andrew G.; Krouse, Donna A.; Isaacs, Natasha M.

doi:10.1016/j.watres.2012.10.006

Cited by 26 publications

(21 citation statements)

References 59 publications

(70 reference statements)

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“…The ability of the indicator data string to predict the presence or absence of a pathogen is expressed as the percentage of samples correctly classified into ''pathogen present'' and ''pathogen absent'' categories (Harwood et al, 2005). An additional measure of accuracy for the Type I relationship was the percentage of cases when the indicator was absent and the pathogen present (Duris et al, 2013).…”

Section: The Datasetmentioning

confidence: 99%

“…A linear combination of indicator concentrations that would give the best separation of samples into groups with and without a pathogen was also sought. The water quality criteria value for E. coli was tested as a threshold to estimate pathogen prevalence in samples above and below it (Duris et al, 2013).…”

Section: The Datasetmentioning

confidence: 99%

“…For example, Listeria monocytogenes is known to survive in multiple habitats, and differences in sources might lead to the negative correlation between E. coli and L. monocytogenes concentrations found by Wilkes et al (2009) in large datasets from the Ontario province. Duris et al (2013) noted that Giardia occurrence was likely related to nonpoint sources which are highly influential during seasonal overland transport from snowmelt and elevated precipitation in late winter and spring in Pennsylvania. It is unknown whether pathogens and E. coli are released from land-deposited animal waste at the same rate, but differences in E. coli and enterococci release were documented (Guber et al, 2007).…”

Section: Ecology and Sourcesmentioning

confidence: 99%

“…(c) Thresholds of prevalence of pathogen markers in a survey in Pennsylvania (Duris et al, 2013) and pathogens in Ontario province, Canada (Wilkes et al, 2009). Differences in transport of E. coli, THT, and pathogens of interest differences may affect the relationship between indicators and pathogens. Lemarchand & Lebaron (2003) and Duris et al (2013), for example, hypothesized that although sources of fecal indicator bacteria and protozoa can be the same, weak correlation to fecal indicator bacteria may be due to differences in settling velocities of protozoa and coliforms and potential differences in entrainment and transport of coliforms and protozoa in soil and in water.…”

Section: Ecology and Sourcesmentioning

confidence: 99%

“…Lemarchand & Lebaron (2003) and Duris et al (2013), for example, hypothesized that although sources of fecal indicator bacteria and protozoa can be the same, weak correlation to fecal indicator bacteria may be due to differences in settling velocities of protozoa and coliforms and potential differences in entrainment and transport of coliforms and protozoa in soil and in water. Brookes et al (2005) noted correlations of Cryptosporidium spp.…”

Section: Ecology and Sourcesmentioning

confidence: 99%

See 4 more Smart Citations

CanE. colior thermotolerant coliform concentrations predict pathogen presence or prevalence in irrigation waters?

Pachepsky

Shelton

Dorner

et al. 2014

Critical Reviews in Microbiology

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An increase in food-borne illnesses in the United States has been associated with fresh produce consumption. Irrigation water presents recognized risks for microbial contamination of produce. Water quality criteria rely on indicator bacteria. The objective of this review was to collate and summarize experimental data on the relationships between pathogens and thermotolerant coliform (THT) and/or generic E. coli, specifically focusing on surface fresh waters used in or potentially suitable for irrigation agriculture. We analyzed peer-reviewed publications in which concentrations of E. coli or THT coliforms in surface fresh waters were measured along with concentrations of one or more of waterborne and food-borne pathogenic organisms. The proposed relationships were significant in 35% of all instances and not significant in 65% of instances. Coliform indicators alone cannot provide conclusive, non-site-specific and non-pathogen-specific information about the presence and/or concentrations of most important pathogens in surface waters suitable for irrigation. Standards of microbial water quality for irrigation can rely not only on concentrations of indicators and/or pathogens, but must include references to crop management. Critical information on microbial composition of actual irrigation waters to support criteria of microbiological quality of irrigation waters appears to be lacking and needs to be collected.

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Section: The Datasetmentioning

confidence: 99%

Section: The Datasetmentioning

confidence: 99%

Section: Ecology and Sourcesmentioning

confidence: 99%

Section: Ecology and Sourcesmentioning

confidence: 99%

Section: Ecology and Sourcesmentioning

confidence: 99%

See 3 more Smart Citations

CanE. colior thermotolerant coliform concentrations predict pathogen presence or prevalence in irrigation waters?

Pachepsky

Shelton

Dorner

et al. 2014

Critical Reviews in Microbiology

View full text Add to dashboard Cite

show abstract

Environmental injustice and Escherichia coli in urban streams: Potential for community‐led response

et al. 2022

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Escherichia coli are fecal indicator bacteria that reach waterbodies through aging and failing infrastructure in cities. Exposure to pathogens in untreated sewage can result in gastrointestinal disease, which frequently goes unreported. In the United States, the Clean Water Act regulates point source discharges of sewage and treated wastewater, but fecal indicator bacteria remain the second leading cause of river impairment. The burden of this contamination is not equitably distributed, with Black, Indigenous, and communities of color suffering from a disproportionate burden of untreated and overflowing waste and subsequent health impacts. Regulatory failures, and even abandonment, along with the exclusion of people of color from the mainstream environmental movement, mean that new approaches are needed to help communities empower themselves to address contamination. Community-led groups that engage residents in addressing issues within and beyond regulatory frameworks have used approaches including trash traps, riparian planting and restoration, and community-led monitoring. Mycofiltration, or the use of fungi to filter pollutants from water, is an emerging and understudied method of remediating E. coli. The low cost of mycofilter installation and maintenance may give agency to communities that have been unduly burdened with sewage contamination to diminish harmful exposures in the near term, even as they continue working toward longer-term regulatory fixes. Continued research is needed to fully understand how different species of mycelium work under varying hydrologic conditions, including in-field installations, along with long-term monitoring and community acceptance to understand the efficacy and the potential impact of this strategy. K E Y W O R D SE. coli, environmental justice, remediation, sewage, urban stream | INTRODUCTIONUrbanized areas, with their high population densities and impervious surfaces, create huge quantities of fecal waste, high organic matter wastewater, and contaminated runoff from both humans and animals that can contaminate surface

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Simultaneous detection of various pathogenic Escherichia coli in water by sequencing multiplex PCR amplicons

Suzuki

Shimizu

Tamai

et al. 2023

Environ Monit Assess

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Waterborne diseases due to pathogen contamination in water are a serious problem all over the world.Accurate and simultaneous detection of pathogens in water is important to protect public health. In this study, we developed a method to simultaneously detect various pathogenic Escherichia coli by sequencing the amplicons of multiplex PCR. Our newly designed multiplex PCR ampli ed ve genes for pathogenic E. coli (uidA, stx1, stx2, STh gene, and LT gene). Additional two PCR assays (for aggRand eae) were also designed and included in the amplicon sequencing analysis. The same assays were also used for digital PCR (dPCR). Strong positive correlations were observed between the sequence read count and the dPCR results for most of the genes targeted, suggesting that our multiplex PCR-amplicon sequencing approach could provide quantitative information. The method was also successfully applied to monitor the level of pathogenic E. coli in river water and wastewater samples. The approach shown here could be expanded by targeting genes for other pathogens.

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Factors related to occurrence and distribution of selected bacterial and protozoan pathogens in Pennsylvania streams

Cited by 26 publications

References 59 publications

CanE. colior thermotolerant coliform concentrations predict pathogen presence or prevalence in irrigation waters?

CanE. colior thermotolerant coliform concentrations predict pathogen presence or prevalence in irrigation waters?

Environmental injustice and Escherichia coli in urban streams: Potential for community‐led response

Simultaneous detection of various pathogenic Escherichia coli in water by sequencing multiplex PCR amplicons

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