Factors of importance when selecting sows as embryo donors

Nohalez, Alicia; Martínez, Cristina A.; Reixach, Josep Peñuelas i; Dı́az, Mònica; Vilà, Jordi; Colina, I.; Parrilla, Inmaculada; Vázquez, José Luís; Roca, Jordi; Gil, M.A.; Rodriguez‐Martinez, Heriberto; Martı́nez, Emilio A.; Cuello, C.

doi:10.1017/s1751731117000325

Cited by 5 publications

(5 citation statements)

References 22 publications

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“…However, other factors, including parity, the weaning‐to‐oestrous interval (WEI) or the season of the year, can decisively influence donor embryo production outcomes and affect the entire ET success. Because there was hardly any information regarding the impact of these factors on embryo production and quality, we investigated whether parity (2 to 7), WEI (oestrus within 3 to 4 days) and season (fall, winter and spring) had any influence on the number of corpora lutea and ovarian cysts, number and quality of collected embryos, embryo developmental stages and recovery and fertilization rates of embryo‐producing donors (Nohalez et al., ). The results of this study showed a lack of significant effects of all variables evaluated or their interactions on any of the reproductive and embryonic parameters examined.…”

Section: Factors Affecting the Et Technologymentioning

confidence: 99%

Achievements and future perspectives of embryo transfer technology in pigs

Martı́nez

Martínez

Cambra

et al. 2019

Reprod Domestic Animals

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Commercial embryo transfer (ET) has unprecedented productive and economic implications for the pig sector. However, pig ET has been considered utopian for decades mainly because of the requirements of surgical techniques for embryo collection and embryo deposition into recipients, alongside challenges to preserve embryos. This situation has drastically changed in the last decade since the current technology allows non-surgical ET and short-and long-term embryo preservation. Here, we provide a brief review of the improvements in porcine ET achieved by our laboratory in the past 20 years. This review includes several aspects of non-surgical ET technology and different issues affecting ET programmes and embryo preservation systems.The future perspectives of ET technology are also considered. We will refer only to embryos produced in vivo since they are the only type of embryos with possible short-term use in pig production. K E Y W O R D Sembryo storage, embryo transfer, porcine, vitrification | 5 MARTINEZ ET Al.

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Section: Factors Affecting the Et Technologymentioning

confidence: 99%

Achievements and future perspectives of embryo transfer technology in pigs

Martı́nez

Martínez

Cambra

et al. 2019

Reprod Domestic Animals

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“…In this experiment, we only used weaned sows of parity 3–7 because they are readily available on farms and because they are usually preferred in ET programs ( 3 ). In addition, unlike prepubertal and mature gilts ( 24 – 27 ), their response to superovulation treatment is very efficient in terms of embryo production and quality ( 11 ), which makes the use of these sows as embryo donors advisable.…”

Section: Discussionmentioning

confidence: 99%

“…However, pig ET has been considered impractical for many years, primarily because of the surgical procedures required to obtain and transfer embryos, in addition to the difficulties in preserving embryos from this species. These circumstances have changed considerably in recent years, as recent advances now allow nonsurgical ET with short-(liquid state) and long-term (vitrified) preserved embryos (1)(2)(3)(4)(5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

A Short-Term Altrenogest Treatment Post-weaning Followed by Superovulation Reduces Pregnancy Rates and Embryo Production Efficiency in Multiparous Sows

et al. 2021

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Although embryo transfer (ET) is a biotechnology ready for the swine industry, there are factors to be solved, the availability of embryo donors as one. Multiparous sows as donors ought to be considered since weaning is a natural and efficient method for estrus synchronization. In addition, superovulation treatments at weaning are effective in increasing the efficiency of donor embryo production. However, ET programs typically require more donors than those available from a single weaning, imposing grouping several weanings to establish a batch for ET. Since short-term administration of Altrenogest is effective in delaying estrus after weaning without effects on ovulation and embryo development, we investigated how Altrenogest combined with superovulation would affect reproductive parameters and embryo quality and quantity of weaned multiparous donor sows. The sows were administered Altrenogest from the day of weaning for 14 (SS-14 group; N = 26), 7 (SS-7 group; N = 31) and 4 (SS-4 group; N = 32) days. The sows were superovulated with eCG 24 h after the last administration of Altrenogest and with hCG at the onset of estrus. Sows not treated with Altrenogest that were superovulated with eCG 24 h post-weaning and hCG at the onset of estrus (SC group; N = 37) and sows with natural estrus after weaning (C group; N = 34) were used as control groups. The percentage of sows showing estrus within 10 days was not affected by the treatment, but the interval from Altrenogest withdrawal to estrus was longer (P < 0.05) in the SS groups than the interval from weaning to estrus in the controls. SS treatments increased (P < 0.05) the percentage of sows with ovarian cysts and the development of polycystic ovaries. The pregnancy and the fertilization rates, and the overall embryo production efficiency were also negatively affected by the SS treatments (P < 0.05). Interestingly, almost 70% of the structures classified as unfertilized oocytes or degenerated embryos in sows from the SS groups were immature oocytes. In conclusion, although superovulation of weaned sows was highly efficient, short-term administration of Altrenogest in combination with superovulation had negative effects on most of the reproductive parameters assessed, particularly affecting the overall efficiency of pregnancy and embryo production.

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“…All sows included in the study were weaned at interval days 21–24 post‐partum, and only those sows with a weaning to oestrus interval of 4 to 5 days were included in the experiment. Sows were selected according to an optimal reproductive history (averaging fertility >90% and litter size >10 piglets) and adequate body condition (2.7 to 3.2 on a five‐point scale on the day of weaning) following the criteria previously (Nohalez et al., 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Neither frozen–thawed seminal plasma nor commercial transforming growth factor‐β1 infused intra‐utero before insemination improved fertility and prolificacy in sows

Parrilla

Rodriguez‐Martinez

Cuello

et al. 2022

Reprod Domestic Animals

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The large, >150 ml, boar ejaculate is to 95% acellular, the composite seminal plasma (SP). Most SP is either discarded during the collection of the different ejaculate fractions, or diluted while preparing semen doses for artificial insemination (AI). Despite having low amounts of SP, farrowing rates and litter sizes equivalent to or even greater using AI than those resulting from natural mating are obtained worldwide (Roca et al., 2016), contrasting with the concept that SP plays an important role in sperm viability and transport and (Crawford et al., 2015), and in later events such as ovulation, corpora lutea formation, fertilization, implantation and subsequent pregnancy (Bromfield, 2014;

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Factors of importance when selecting sows as embryo donors

Cited by 5 publications

References 22 publications

Achievements and future perspectives of embryo transfer technology in pigs

Achievements and future perspectives of embryo transfer technology in pigs

A Short-Term Altrenogest Treatment Post-weaning Followed by Superovulation Reduces Pregnancy Rates and Embryo Production Efficiency in Multiparous Sows

Neither frozen–thawed seminal plasma nor commercial transforming growth factor‐β1 infused intra‐utero before insemination improved fertility and prolificacy in sows

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