Factors Limiting Aliphatic Chlorocarbon Degradation by
            <i>Nitrosomonas europaea</i>
            : Cometabolic Inactivation of Ammonia Monooxygenase and Substrate Specificity

Rasche, Madeline E.; Hyman, Michael R.; Arp, Daniel J.

doi:10.1128/aem.57.10.2986-2994.1991

Cited by 171 publications

(113 citation statements)

References 23 publications

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“…It is well known that enzymatic transformation of certain compounds can result in deleterious metabolic effects.2.1S, 18,28,34,42 However, the distinct mechanisms of inhibition and inactivation have not been modeled entirely satisfactorily to date. Competition for the primary enzyme of interest has been noted p r e v i o u~l y ,~~ and models have been proposed in which enzyme inactivation is expressed in terms of decreases in "active biomass.…”

Section: Discussionmentioning

confidence: 99%

“…( 2 ) oxidation of the nongrowth substrate consumes reductant that must be replaced e n d o g e n~u s l y "~~~; and (3) oxidation of the nongrowth substrate can yield products toxic to the monooxygenase enzyme and other proteins. 16,34 However, evidence suggests that cells may be able to counter these inhibiting and inactivating effects under some circumstances. Bacteria are known to synthesize particular proteins in response to heat shock and other s t r e s~e s .~'~~ In addition, it has been shown that ammonia-oxidizing bacteria can recover from TCE toxicity, in the absence of TCE, by de novo enzyme synthesis.18934 Recovery potential in the presence of TCE has not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

“…Finally, as discussed earlier, 8 is the ratio of the pseudo-first-order reaction rates of the growth substrate and nongrowth substrate, respectively. Therefore, dimensionless Eq (34). indicates that the fraction of maximum growth substrate activity ( d d d7 will be equal to the extent of enzyme saturation with the growth substrate times one minus the fraction of activity lost plus the fraction of activity recovered, all divided by one plus the extent of enzyme saturation with the growth substrate plus the extent of enzyme saturation with the nongrowth substrate.…”

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confidence: 99%

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A cometabilic kinetics model incorporating enzyme inhbition, inactivation, and recovery: I. Model development, analysis, and testing

Ely

Williamson

Guenther

et al. 1995

Biotech & Bioengineering

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Cometabolic biodegradation prcesses are important for bioremediation of hazardous waste sites. However, these proceeses are not well understood and have not been modeled thoroughly. Traditional Michaelis-Menten kinetics models often are used, but toxic effects and bacterial responses to toxicity may cause changes in enzyme levels, rendering such models inappropriate. In this article, a conceptual and mathematical model of cometabolic enzyme kinetics i described. Model derivation is based on enzyme/growth-substrate/nongrowth-substrate interaction and incorporates enzyme inhibition (caused by the presence of a cometabolic compound), inactivation (resulting from toxicity of a cometabolic product), and recovery (associated with bacterial synthesis of new enbzyme in response to inactivation). The mathematical model consists of a system of two, nonlinear ordinary differential equations that can be solved implicitly using numerical methods, providing estimates of model parameters. Model analysis shows that growth substraate adn nongrowth substrate oxidation rates are related by a dimensionless constant. Reliability of tehy model solution prcedure is verifiedl by abnalyzing data ses, containing random error, from simulated experimentss with trichhloroethyylene (TCE) degradation by ammonia-oxidizing bacterialunder various conditions. Estimation of the recovery rate contant is deterimined to be sensitive to intial TCE concentration. Model assumptions are evaluated in a companion article using data from TCE degradation experiments with amoniaoxidizing bacteria. (c) 1995 John Wiley & Sons, Inc.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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A cometabilic kinetics model incorporating enzyme inhbition, inactivation, and recovery: I. Model development, analysis, and testing

Ely

Williamson

Guenther

et al. 1995

Biotech & Bioengineering

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“…However, many other organic contaminants, including many herbicides, have no effect on nitrification [14]. Rasche et al [15] demonstrated that ammonia oxidation to nitrite by Nitrosomonas europea in pure culture is inhibited by several chlorinated solvents due to an interaction with the ammonia monooxygenase enzyme (AMO). It was found that if sufficient time was allowed to elapse for de novo protein synthesis, nitrification activity could be reestablished.…”

Section: Discussionmentioning

confidence: 99%

Effect of Application Solvents on Heterotrophic and Nitrifying Populations in Soil Microcosms

Miller

Sardo

Thompson

et al. 1997

Environ Toxicol Chem

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Agricultural practices may cause contamination of soil and ground water with a combination of organic compounds (pesticides and fuel) and nitrogen fertilizers. In coupled microcosm studies that monitored the mineralization of naphthalene and the nitrification of ammonia, it was observed that the solvent (dichloromethane) used to apply naphthalene to the soil inhibited nitrification, although there was no effect on naphthalene mineralization. Further studies were performed with a series of application solvents: methanol, acetonitrile, trichloromethane, and dichloromethane. Soil and solvent were allowed to equilibrate with ambient air for various times before capping and incubation of microcosms. Results indicated that dichloromethane equilibrated for 5 mins inhibited nitrification for at least 3 weeks relative to the control (water). Acetonitrile and trichloromethane similarly inhibited nitrification. Methanol and dichloromethane equilibrated for 60 mins also significantly delayed nitrification, although to a lesser extent. Inhibition of nitrification was not permanent, and nitrification activity was eventually restored in all systems tested. None of the solvents inhibited mineralization of the added carbon source. These results indicate that special care must be taken to ensure that applications solvents do not affect the activity of sensitive microbial populations, such as the nitrifiers, that may be part of a study.

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“…The inhibitory effect of aniline, one of the most important amines produced, is thought to be caused by competition with ammonia for the active site of the enzyme AMO. A key enzyme, AMO is known to be inhibited by a variety of chemicals such as aromatic compounds, aliphatic chlorocarbon, ethylene, chloroethane, and 1-1-1 trichloroethane (Hyman and Wood, 1984;Arp, 1993, 1994;Rasche et al, 1990Rasche et al, , 1991.…”

Section: Model Developmentmentioning

confidence: 99%

Modeling of Nitrification Inhibition with Aniline in Suspended‐Growth Processes

Khin

Gheewala²,

Annachhatre³

2002

Water Environment Research

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Nitrification inhibition due to aniline was investigated in completely mixed suspended-growth, batch, and continuous processes. Synthetic wastewater was used with aniline as the carbon source. The experiments were conducted at aniline concentrations inhibitory to nitrifier organisms. In the batch tests, degradation took place rapidly (within 4 to 6 hours) for initial aniline concentrations below 100 mg/L, with nitrification picking up as soon as the aniline concentration decreased to less than 3 to 4 mg/L. For initial aniline concentrations of 250 mg/L and higher, complete nitrification did not take place even though the aniline concentration decreased to less than 3 to 4 mg/L. This observation indicated nitrifier inhibition due to aniline. In the continuous experiments, a hydraulic residence time of 8 to 24 hours and a solids retention time of 8 to 24 days were maintained. Complete nitrification took place at bulk aniline concentrations less than 0.5 mg/L, while at higher aniline concentrations, nitrification inhibition took place. The inhibitory effect of aniline on the nitrification process was modeled using uncompetitive inhibition kinetics. Modeling of batch processes yielded the value for the inhibition constant for aniline, K i , as 3.3 mg/L. Modeling of continuous processes yielded criteria for stable process operation for nitrification under inhibitory conditions, which were also confirmed through experimental results. Water Environ. Res., 74, 531 ( 2002).

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Factors Limiting Aliphatic Chlorocarbon Degradation by Nitrosomonas europaea : Cometabolic Inactivation of Ammonia Monooxygenase and Substrate Specificity

Cited by 171 publications

References 23 publications

A cometabilic kinetics model incorporating enzyme inhbition, inactivation, and recovery: I. Model development, analysis, and testing

A cometabilic kinetics model incorporating enzyme inhbition, inactivation, and recovery: I. Model development, analysis, and testing

Effect of Application Solvents on Heterotrophic and Nitrifying Populations in Soil Microcosms

Modeling of Nitrification Inhibition with Aniline in Suspended‐Growth Processes

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