Factors involved in control of tissue-specific expression of albumin gene

Cereghini, Silvia; Raymondjean, Michel; Carrancá, Alejandro García; Herbomel, Philippe; Yaniv, Moshe

doi:10.1016/0092-8674(87)90036-5

Cited by 360 publications

(240 citation statements)

References 29 publications

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“…The NF-I is a family of proteins generally regarded as ubiquitous transcriptional factors for various cellular and viral genes [45][46][47]. This proteins family is critical for the expression of liver-specific [48,49] and brain-specific genes [50], for activation of adipocyte-specific enhancer [51], and for epithelial cellspecific transcription [52]. In most cases, NF-I proteins cooperate with other ubiquitous or tissue-specific TFs whose binding sites are in close proximity.…”

Section: Identification Of Transcription Factor Binding Sitesmentioning

confidence: 99%

Polymorphisms of beta-lactoglobulin promoter region in three Sicilian goat breeds

et al. 2011

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Several beta-lactoglobulin (BLG) polymorphisms have been described within the proximal promoter region and coding region of the caprine gene, although no genetic variants affecting the protein amino acid composition and/or expression level have been characterized so far. Binding sites for several transcription factors (TFs) are present in the BLG promoter region. The aims of this work were to sequence the full-length promoter region of three Sicilian goat breeds in order to identify polymorphisms, analyze the identified haplotypes, search for differences between breeds for the presence of polymorphisms in this gene region, search for putative TFs binding sites, and check if polymorphisms lay within the identified TFs binding sites. The promoter region of BLG gene in Sicilian goat breeds showed high level of polymorphism due to the presence of 36 single nucleotide polymorphisms (SNPs). Association between polymorphic sites was computed within the whole sample analyzed and 18 haplotypes were inferred. Binding sites for three milk protein binding factors (MPBFs) and four nuclear factor-I (NF-I) were found within BLG promoter region based on the ovine sequence. The identification of some SNPs within TFs binding sites allowed hypothesizing the loss of TFs. Further studies are in progress to evaluate the effect of these mutations on binding affinity of TFs, the functional interaction of the TFs with the goat BLG promoter, and the relationship of the polymorphisms with BLG gene expression and milk production and composition.

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Section: Identification Of Transcription Factor Binding Sitesmentioning

confidence: 99%

Polymorphisms of beta-lactoglobulin promoter region in three Sicilian goat breeds

et al. 2011

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“…These genes lack any predicted perfect NFI dyad symmetric binding sites. While some mammalian promoters have been shown to contain functional hemi NFI binding sites (a single TTGGC or GCCAA site alone) (Cereghini et al, 1987;BoisJoyeux and Danan, 1994;Alonso et al, 1996;Gao et al, 1996), the absence of high-affinity dyad symmetric NFI binding sites within the myosin and exp-2 genes raises the possibility that they may not be direct targets of NFI.…”

Section: Fig 2 Amentioning

confidence: 99%

Lifespan extension and increased pumping rate accompany pharyngeal muscle‐specific expression of nfi‐1 in C. elegans

Lazakovitch

Kalb

Gronostajski

2008

Developmental Dynamics

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Caenorhabditis elegans nfi-1 belongs to the Nuclear Factor I (NFI) family of transcription factors known to regulate metazoan gene expression and development. We showed previously that loss of nfi-1 in worms results in multiple behavioral defects; slower pharyngeal pumping rate, impaired egg laying, defective motility, and a shortened life span. Here, we generated cell-type specific transgenic worms to determine the cells in which nfi-1 must be expressed to rescue the pharyngeal pumping defect. Expression of nfi-1 from the pharyngeal muscle-specific myo-2 promoter, but not from the F25B3.3 or myo-3 promoters, rescued the pharyngeal pumping defect of nfi-1 worms. Surprisingly, myo-2-driven nfi-1 expression also rescued the shortened lifespan of nfi-1 worms, demonstrating a possible cell-autonomous role of nfi-1 in pharyngeal muscle for both phenotypes. We propose some relationships between the pharyngeal pumping and lifespan phenotypes and potential mechanisms of nfi-1 function.

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“…Nuclear and whole-cell extracts from mouse embryo, testes and spermatogenic cells were prepared as described elsewhere (Cereghini et al, 1987). All procedures were performed at 48C.…”

Section: Protein Extraction and Gel-shift Assaysmentioning

confidence: 99%

Gli family members are differentially expressed during the mitotic phase of spermatogenesis

et al. 1997

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The Gli family of DNA binding proteins has been implicated in multiple neoplasias and developmental abnormalities, suggesting a primary involvement in cell development and di erentiation. However, to date their speci®c roles and mechanisms of action remain obscure, and a drawback has been the lack of a model system in which to study their normal function. Here we demonstrate that Gli family members are di erentially expressed during spermatogenesis in mice. Speci®cally, Gli and Gli3 mRNAs were detected in mouse germ cells, while Gli2 was not. Further, both Gli and Gli3 exhibited stage-dependent patterns of expression selectively in type A and B spermatogonia. Gli expression was somewhat higher in type B spermatogonia while the abundance of Gli3 transcripts was similar in type A and B cells. Gel-shift analyses also demonstrated the enrichment of DNA binding activity speci®c for the Gli target sequence in spermatogonial cells. These results indicate a selective role for Gli and Gli3 during mitotic stages of male germ cell development. Spermatogenesis may thus provide a unique opportunity to identify downstream targets and explore the normal function of Gli family proteins.

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Factors involved in control of tissue-specific expression of albumin gene

Cited by 360 publications

References 29 publications

Polymorphisms of beta-lactoglobulin promoter region in three Sicilian goat breeds

Polymorphisms of beta-lactoglobulin promoter region in three Sicilian goat breeds

Lifespan extension and increased pumping rate accompany pharyngeal muscle‐specific expression of nfi‐1 in C. elegans

Gli family members are differentially expressed during the mitotic phase of spermatogenesis

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