Factors involved in Bacillus spore's resistance to cold atmospheric pressure plasma

Hertwig, Christian; Reineke, Kai; Rauh, Cornelia; Schlüter, Oliver

doi:10.1016/j.ifset.2017.07.031

Cited by 34 publications

(24 citation statements)

References 44 publications

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“…To measure the amount of plasma active species, the air flow of the internal suction pump was set to 25 lpm and the power setting value (time) was increased from 50% (5 s on/5 s off) to 100% (10 s on/10 s off). As shown in Figure 1 and [20] found that there was a significant difference in plasma emission intensity depending on the gas types (dry air, N 2 , O 2 , and CO 2 ). N 2 as a process gas showed the highest emission intensity compared to the other process gasses.…”

Section: Active Species Generated By Corona Discharge Air Plasmamentioning

confidence: 79%

Microbial Decontamination of Onion by Corona Discharge Air Plasma during Cold Storage

Chang

Bae²,

Shin

et al. 2018

Journal of Food Quality

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Corona discharge air plasma (CDAP) is a nonthermal decontamination technology which is generating antimicrobial agents such as photons, electrons, positively and negatively charged ions, atoms, and free radicals. We investigated the effect of a corona discharge under atmospheric pressure on the sterilization of postharvest fungal pathogens on onion. The main antimicrobial reactive substance generated by CDAP was O3. The active species such as nitric oxide (NO) and nitric dioxide (NO2) were nearly detected in this experiment. CDAP treatment revealed different isolation frequencies depending on postharvest pathogens from diseased onions, showing less isolation frequency of Fusarium spp. and Alternaria sp. than that of Botrytis spp. when compared with untreatment onions during 10-month cold storage. CDAP treatment at 2∼2.6 ppm of O3 slightly stimulated the mycelial growth of Alternaria sp., while the treatment at 20∼24 ppm of O3 gradually inhibited mycelial growth by treatment time. However, Botrytis sp. showed different patterns of mycelial growth with CDAP treatment. Less than 4 hours’ treatment of CDAP slightly inhibited the mycelial growth of Botrytis sp., while 8 hours’ treatment of CDAP slightly stimulated the mycelial growth of Botrytis sp. not depending on the concentration of O3. The inhibitory effect of CDAP on the conidial germination of Alternaria sp. and Botrytis sp. was examined with treatment time and intensity of CDAP. The conidial germination of Alternaria sp. treated with CDAP at the concentration of 13.7∼14.4 ppm of O3 was strongly inhibited by time, showing y = 2.66x2 − 85.139x + 4.88 and R2 = 0.98. When the conidia of Alternaria sp. were exposed for 2 hours with varying plasma O3 concentration, the conidial germination was strongly inhibited as the concentration of O3 increases, showing y = −0.09x2 + 6.905x − 0.764 and R2 = 0.95. The conidia of Botrytis sp. also showed similar patterns to CDAP. The inhibitory effect of CDAP on the germination of postharvest pathogens depends on treatment time and O3 concentration.

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Section: Active Species Generated By Corona Discharge Air Plasmamentioning

confidence: 79%

Microbial Decontamination of Onion by Corona Discharge Air Plasma during Cold Storage

Chang

Bae²,

Shin

et al. 2018

Journal of Food Quality

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“…found that there were almost no UV‐C or UV‐B generated and the presence of primarily UV‐A photons contributed minimally to spore inactivation, although the charged particles and reactive oxygen species contributed significantly. Hertwig et al . demonstrated that non‐thermal atmospheric pressure plasma of air generated much less UV‐C comparing to that of nitrogen gas, and the inactivation rate was much faster using the nitrogen‐plasma, although there was no reactive oxygen species present in the nitrogen‐plasma.…”

Section: Resultsmentioning

confidence: 99%

“…Using NTP-AA, Wang et al 9 found that there were almost no UV-C or UV-B generated and the presence of primarily UV-A photons contributed minimally to spore inactivation, although the charged particles and reactive oxygen species contributed significantly. Hertwig et al 10 demonstrated that non-thermal atmospheric pressure plasma of air generated much less UV-C comparing to that of nitrogen gas, and the inactivation rate was much faster using the nitrogen-plasma, although there was no reactive oxygen species present in the nitrogen-plasma. Further research with respect to designing efficient spore inactivation processes should not only consider the characteristics of NTP and the energy consumption, but also the structure and biochemical properties of spores and the reactions of spore components to different species in NTP.…”

Section: H Nmr Analysis Of Dpamentioning

confidence: 99%

“…NTP technology is considered efficient and economically viable as a result of its relatively low operating cost and energy consumption, with actual or potential applications with respect to inactivating microorganisms on foods such as almonds, fresh produce, food processing equipment and food handling environments, as well as in heat‐sensitive packaging materials, pharmaceuticals and medical devices . Because of its efficiency and ease for implementation, non‐thermal atmospheric pressure plasma, with different configurations and discharge gases, has been the focus of recent studies investigating the inactivation of spores …”

Section: Introductionmentioning

confidence: 99%

“…7,8 Because of its efficiency and ease for implementation, non-thermal atmospheric pressure plasma, with different configurations and discharge gases, has been the focus of recent studies investigating the inactivation of spores. [9][10][11][12][13] Despite extensive research, the applications of NTP to microbial control remain poorly understood, and the diversity of the devices and complexity of NTP generation make it difficult to allow any general evaluation of the potential of the NTP technologies. 14 Moreover, additional considerations are needed when inactivating resistant bacterial spores.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An investigation of inactivation mechanisms of Bacillus amyloliquefaciens spores in non‐thermal plasma of ambient air

Huang

Doona

et al. 2018

J Sci Food Agric

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Progressive erosion of spore surface by NTP-AA with ensuing or concomitant biochemical damage, which includes the alteration of structural proteins, internal lipids and the loss of dipicolinic acid content from the spore core, represent the main mechanisms of inactivation, and there is evidence that reactive NTP-AA species could penetrate the cortex and reach the core of spores to cause damage. © 2018 Society of Chemical Industry.

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Efficiency of plasma‐processed air for biological decontamination of crop seeds on the premise of unimpaired seed germination

Wannicke

Wagner

Stachowiak

et al. 2020

Plasma Processes & Polymers

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In this study, the antimicrobial effect of plasma‐processed air (PPA) generated by a microwave‐induced nonthermal plasma was investigated for preharvest utilization using three crop species: Barley, rape, and lupine. Bacillus atrophaeus spores were chosen as a model, inoculated onto seeds, and subsequently treated with PPA at two different flow rates, different filling regimes, and gas exposure times. PPA treatment was efficient in reducing viable spores of B. atrophaeus, reaching sporicidal effects in all species at certain parameter combinations. Maximum germination of seeds was strongly reduced in barley and rape seeds at some parameter combination, whereas it had a modest effect on lupine seeds. Seed hydrophilicity was not altered. Overall, PPA investigated in this study proved suitable for preharvest applications.

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Factors involved in Bacillus spore's resistance to cold atmospheric pressure plasma

Cited by 34 publications

References 44 publications

Microbial Decontamination of Onion by Corona Discharge Air Plasma during Cold Storage

Microbial Decontamination of Onion by Corona Discharge Air Plasma during Cold Storage

An investigation of inactivation mechanisms of Bacillus amyloliquefaciens spores in non‐thermal plasma of ambient air

Efficiency of plasma‐processed air for biological decontamination of crop seeds on the premise of unimpaired seed germination

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