Factors influencing the production of endopolysaccharide and exopolysaccharide from Ganoderma applanatum

Lee, Wi Young; Park, Youngki; Ahn, Jin Kwon; Ka, Kang Hyeon; Park, So Young

doi:10.1016/j.enzmictec.2006.04.009

Cited by 92 publications

(60 citation statements)

References 30 publications

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“…Carbon and nitrogen sources play a significant role in determining the production level of submerged fermentation because these nutrients are directly linked with cell proliferation and metabolite biosynthesis (Kim et al, 2003;Zou, 2005). Maltose has been found to be best carbon source for the cell mass and EPS production in G. applanatum (Lee et al, 2007) and G. lucidum (Tang and Song, 2002), respectively, because it can be easily used for biosynthesis of biomass and EPS, as glucose is the main sugar component of the cellular energy production system and EPS. Skim milk is substrate for protease enzymes, such as fibriolytic enzymes (Silva et al, 2003;Korat and Rizvi, 2004;Albillos et al, 2007;Merheb et al, 2007), which Ganoderma species have the ability to (Jo et al, 2011;Kumaran et al, 2011) in order to digest complex protein molecules into simple polypeptide chains and amino acids (Sharma et al, 2011) for the growth and metabolic activities.…”

Section: Discussionmentioning

confidence: 99%

COMBINATION OF STATISTICAL TECHNIQUES FOR SUBMERGED FERMENTATION FOR EXTRACELLULAR POLYSACCHARIDE AND BIOMASS OF <I>GANODERMA TSUGAE</I>

Nukrob¹

2013

American Journal of Biostatistics

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Biomass and extracellular polysaccharide of Ganoderma tsugae have various biological activity including anti-inflamatory activity, antioxidant activity and antitumor activity. However, the growth rate of G. tsugae in nature is very slow. Therefore, many studies have attempted to develop mass culture systems for G. tsugae using laboratory techniques. Many parameters of submerged fermentation for G. tsugae were studies to determine the optimization of process by combination of statistical techniques. Ten parameters from preliminary results and literature reviews (maltose, skim milk, KH 2 PO 4 +K 2 HPO 4 , MgSO 4 -7H 2 O, CaCO 3 , vitamin B 5 +B 6 , olive oil, ethanol, pH and shaking speed) were screened by Packett Berman design. The significant parameters were determined the optimal ranges by path of steepest ascent method. The optimal condition of process was performed by response surface method. Maltose, skim milk and pH are significant parameters for G. tsugae cultivation. The conditions of 31.031 g L −1 maltose, 14.055 g L −1 skim milk and an initial pH of 7.12 resulted in the maximum extracellular polysaccharide content of 415 mg L −1 and the same fermentation broth at an initial pH of 6.46 exhibited the most biomass at 15.776 g L −1 . Finally, the optimal condition was compared with un-optimal condition which result indicates that the combination of statistical techniques enhance the productions of biomass and extracellular polysaccharide (13X and 1.5X of the control, respectively). Therefore, these strategies are useful for improvement of submerged fermentation of G. tsugae which it can apply in pharmaceutical industry.

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Section: Discussionmentioning

confidence: 99%

COMBINATION OF STATISTICAL TECHNIQUES FOR SUBMERGED FERMENTATION FOR EXTRACELLULAR POLYSACCHARIDE AND BIOMASS OF <I>GANODERMA TSUGAE</I>

Nukrob¹

2013

American Journal of Biostatistics

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“…The mycelia isolated from each mushroom were maintained on the potato dextrose agar (PDA) medium. Each mycelium grown on PDA was inoculated to 100 ml fungal growth medium (FGM) [22] and then grown at 25°C on a shaking incubator at 110 rpm for 10 days. This preculture was then homogenized at 13,000 rpm for 8 s in a homogenizer (Ingenieurbüro CAT.…”

Section: Methodsmentioning

confidence: 99%

Supplementation of Methionine Enhanced the Ergothioneine Accumulation in the Ganoderma neo-japonicum Mycelia

Lee¹,

Park²,

Ahn³

2008

Appl Biochem Biotechnol

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The methods for increasing the production of ergothioneine (ERG) were investigated by using the mycelial culture of several mushroom species, primarily Ganoderma neo-japonicum. We first found that ERG was accumulated at the different levels in mycelia and fruiting bodies, respectively, depending on the mushroom species. As a result of adding various amino acids to the mycelial culture medium, methionine (Met) was shown to be the most effective additive. The most preferable condition of the additive was the combination of 4 mM Met and 1 g/l of yeast extract, and the maximum ERG production reached approximately 1.7 mg/l, which corresponds to 2.4 times (0.7 mg/l) that in the basal medium without Met. Although the supplementation of Met enhanced the ERG production, the mycelial growth was significantly inhibited. Furthermore, the analysis of amino acids in the culture medium revealed that the Met additive reduced the consumption rates of most amino acids tested, probably due to the decrease in mycelial growth. Taking these results into consideration, we suggest that the addition of Met to the mycelial culture medium is an efficient way to enhance the ERG production in economically important mushroom species.

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“…ALRs have been tested for production of biopolymers from fungi Ganoderma applanatum (440), Tremella fuciformis (441), Paecilomyces tenuipes (442), Grifola frondosa (443), and Sclerotium glucanicum (444). κ-Carrageenan for the phycocolloid industry may also be obtained from Kappaphycus alvarezii calluses in a DT-ALR (445).…”

Section: Biopolymersmentioning

confidence: 99%

Bioreactors: Airlift Reactors

Merchuk

Camacho

2010

Encyclopedia of Industrial Biotechnology

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ALRs are popular in modern bioprocess research and development and, in many cases, they have found industrial uses. The distinctive characteristics of ALRs are conferred by the fluid dynamics of the liquid‐gas or liquid gas‐solid mixtures: holdup, liquid velocity, and mixing in the riser, gas separator, and downcomer. Only a correct understanding of the interconnection of these regions can make possible the scale‐up of a laboratory device, to pilot or industrial size. Several correlations are available in the literature for the prediction of the fluid dynamic characteristics of the reactor and of the mass and heat transfer coefficients, and a selection is presented in this review. Significant progress has been made in the application of modern computational tools to the simulation and design of ALRs. In parallel, innovative and sophisticated methods have been applied to obtain a deeper insight into the mechanisms of fluid motion and the flow patterns in the reactor. However, no single research group has managed to cover all the variables over a wide range. The engineer confronting scale‐up or de novo design of an ALR must analyze the validity of the correlations and computation methods used for the calculations.

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Factors influencing the production of endopolysaccharide and exopolysaccharide from Ganoderma applanatum

Cited by 92 publications

References 30 publications

COMBINATION OF STATISTICAL TECHNIQUES FOR SUBMERGED FERMENTATION FOR EXTRACELLULAR POLYSACCHARIDE AND BIOMASS OF <I>GANODERMA TSUGAE</I>

COMBINATION OF STATISTICAL TECHNIQUES FOR SUBMERGED FERMENTATION FOR EXTRACELLULAR POLYSACCHARIDE AND BIOMASS OF <I>GANODERMA TSUGAE</I>

Supplementation of Methionine Enhanced the Ergothioneine Accumulation in the Ganoderma neo-japonicum Mycelia

Bioreactors: Airlift Reactors

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