Factors influencing the fate of antibiotic resistance genes during thermochemical pretreatment and anaerobic digestion of pharmaceutical waste sludge

Abstract

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Disentangling the Effects of Physicochemical, Genetic, and Microbial Properties on Phase-Driven Resistome Dynamics during Multiple Manure Composting Processes

“…Furthermore, the link between microbial phylogeny and the resistome was supported by Procrustes analyses, as the resistome correlated significantly with microbial communities during composting (sum of squares M 2 = 0.3578–0.6486, P < 0.001, based on 9999 permutations, Figure S10), along with correlation analyses, as the absolute abundances of ARGs correlated significantly with those of 16S rRNA genes during composting (Pearson r = 0.9390, P < 0.001, Figure S2). These findings further supported the notions that the resistome profile during composting is largely dependent on the microbial genotype and that a greater microbial diversity enhances the dissemination of ARGs . In order to more specifically investigate the association between ARGs and microbial communities during composting, we constructed co-occurrence networks that were, for the first time, based on ARG datasets and a wide range of microbial communities (bacterial, fungal, and protistan).…”

“…These findings further supported the notions that the resistome profile during composting is largely dependent on the microbial genotype 16 and that a greater microbial diversity enhances the dissemination of ARGs. 88 In order to more specifically investigate the association between ARGs and microbial communities during composting, we constructed co-occurrence networks that were, for the first time, based on ARG datasets and a wide range of microbial communities (bacterial, fungal, and protistan). Due to differences in the variation of persistent and transient ARGs, ARG−microbe networks were evaluated separately for these two datasets (Figure 5a).…”

An overview of the occurrence, impact of process parameters, and the fate of antibiotic resistance genes during anaerobic digestion processes

“…Literature reports demonstrated that most ARGs and MGEs were reduced after the pretreatment. However, their abundances increased again after the AD process which is called the rebound effect (Ma et al 2011 ; Pei et al 2016 ; Tong et al 2018 , 2019 ; Wang et al 2019a ; Sun et al 2019a ). Since the treatment significantly removes ARGs in the feedstock, the increase of resistance genes during AD could be attributed to the seed inoculum used in the systems (Ma et al 2011 ; Pei et al 2016 ).…”

Post-digestate composting shifts microbial composition and degrades antimicrobial resistance genes

“…Moreover, mitigating the spread of ARGs in the environment is essential to reduce potential health risks. There are several pathways through which ARGs can be released to the environment such as manure land application (Tien et al, 2017), disposal of poorly treated pharmaceutical sludge (Tong et al, 2018), and digestate land application (Derongs et al, 2020).…”