“…An increased diversity and reduced stability of members of the Clostridia supports previously demonstrated associations between CC and increased counts of Clostridia using culture dependent methods (Kanazawa et al, 1996) and a recent culture-independent analysis of 7-a dehydroxylating Clostridia (7DHC) . Furthermore, recent culture-independent studies have highlighted significant alterations in the community structure of the Clostridia in various inflammatory diseases of the GI tract (Manichanh et al, 2006).…”
Section: Discussionsupporting
confidence: 82%
“…The contribution the GI microbiota make to CC progression and development is unclear and complex (Kanazawa et al, 1996;O'Keefe et al, 2007). This exploratory study highlights the differences in both the microbial community structure and stability in CC and PP compared with controls.…”
A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture-independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.
“…An increased diversity and reduced stability of members of the Clostridia supports previously demonstrated associations between CC and increased counts of Clostridia using culture dependent methods (Kanazawa et al, 1996) and a recent culture-independent analysis of 7-a dehydroxylating Clostridia (7DHC) . Furthermore, recent culture-independent studies have highlighted significant alterations in the community structure of the Clostridia in various inflammatory diseases of the GI tract (Manichanh et al, 2006).…”
Section: Discussionsupporting
confidence: 82%
“…The contribution the GI microbiota make to CC progression and development is unclear and complex (Kanazawa et al, 1996;O'Keefe et al, 2007). This exploratory study highlights the differences in both the microbial community structure and stability in CC and PP compared with controls.…”
A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture-independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.
“…In comparison to the sulfur concentration in the periodontal pocket, much higher concentration of H 2 S, over 1000 ppm greater than the lethal dose exists in the colon [40][41][42]. Colonic epithelium cope with this toxicity by methylation and de-methylation [42,43].…”
Section: Carcinogenicity Of Vscsmentioning
confidence: 99%
“…Incubation of colonic mucosa with H 2 S produced significant damage including apoptosis, loss of goblet cells, and superficial ulcerations [49]. H 2 S concentration in feces from subjects at high risk of sigmoid colon cancer was significantly higher than in the control group [41]. Proliferation of colonic mucous cells was accelerated by H 2 S, and controlled by the Ras family that consists of Ha-ras, Ki-ras, and N-ras genes [50,51].…”
“…42,43 In another study, lecithinase-negative Clostridium and Lactobacillus were more abundant in colon cancer patients. 44 Some Lactobacillus species and Eubacteriumaerofaciens have been associated with a reduced risk. 43 In animals, colonic tumour formation is dependent on the presence of intestinal flora.…”
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