Factors Influencing Quantitative Determination of Methylpentoses and Ketohexoses with Anthrone

Helbert, J.; Brown, Keith

doi:10.1021/ac60107a034

Cited by 46 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this procedure the rhamnose was first determined in one sample of carbohydrate by the l0 minute Dische reaction. Another sample was then tested by the anthrone test (7). The optical density of the test reaction for the carbohydrate is a summation due to both the rhamnose and the galactose content.…”

Section: G Streptococcimentioning

confidence: 99%

Immunochemical Studies on the Specific Carbohydrate of Group G Streptococci

Curtis

Krause

1964

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Although Group G streptococci have been recovered frequently from various sources including the human nasopharynx and the serological classification has been firmly established (1), little attention has been directed to the distinctive immunochemical features of the group-specific carbohydrate antigen. In the present study, therefore, the Group G carbohydrate purified from isolated cell walls, has been characterized and a major chemical determinant of antigenic specificity has been identified.In certain respects the chemical content of the cell walls and carbohydrate antigens of Group G streptococci resembles that of Groups A, A-variant, and C streptococci. The trypsinized cell walls of the latter Groups of streptococci contain two major components: a mucopeptide matrix consisting of N-acetylmuramic acid, N-acetylglucosamine, and four amino acids; and the groupspecific carbohydrate, exterior to the mucopeptide, which is composed of rhamnose and N-acetylhexosamine (2, 3). Data to be reported here indicate that the constituent sugars of Group G carbohydrate are rhamnose, galactosamine, and galactose while the composition of the mucopeptide is similar to that of the other three groups of streptococci. Materials and MethodsStreptococcal Strains.--Group G cell walls were prepared from strains D166B, B763A, and B549 obtained from Dr. Rebecca C. Lancefield, The Rockefeller Institute. Preparation of Cell Walls and C-roup-SpeciJ~Carbohydrate.--Cell walls were prepared by previously described methods (4). The group carbohydrate was extracted from the cell walls by the formamide procedure (5).Analytical Methods.--Analyses for rhamnose, muramic acid, glucosamine, galactosamine, and the amino acids were performed by previously described methods (3, 4).

show abstract

Section: G Streptococcimentioning

confidence: 99%

Immunochemical Studies on the Specific Carbohydrate of Group G Streptococci

Curtis

Krause

1964

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…Substituted calyx [8] Membrane transport was studied in a U-shaped tube (d = 19 ram). The membrane was a solution of a reagent-"host" in chloroform (V = 20 mL, C = 1 9 10 -3 mol L-l); an aqueous phase: a solution of sugar (V = 10 mL, C = 0.1 tool L -I) was used as a source solution and distilled water (V = 10 mL) was used as a receiving solution.…”

Section: Methodsmentioning

confidence: 99%

“…The membrane was a solution of a reagent-"host" in chloroform (V = 20 mL, C = 1 9 10 -3 mol L-l); an aqueous phase: a solution of sugar (V = 10 mL, C = 0.1 tool L -I) was used as a source solution and distilled water (V = 10 mL) was used as a receiving solution. The content of sugar in the receiving solution was controlled photometrically using a reaction with anthrone, s Substituted calyx [8]arenes 1 and 2 transport monosaccharides (and not disaccharides) across a chloroform membrane. The selectivity of the two reagents is different (Table I).…”

Section: Methodsmentioning

confidence: 99%

“…

Key words: transport across a bulk liquid membrane, "host--guest" complexation; substituted calyx [S]arenes, podands; monosaccharides, disaccharides.

Needs of biotechnology and pharmacology motivate interest in novel synthetic reagents for binding polyhydroxy compounds, in particular, monosaccharides and their derivatives.

…”

mentioning

confidence: 99%

“…For example, transport of sugars with derivatives of boric acid has been studied, but this case does not deal with "host--guest" complexation, a Earlier, we used substituted calyx [8]arenes and podands for binding amines. 7 Those reagents are lipophilic and contain functional groups capable of forming hydrogen bonds with sugars.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Transport of carbohydrates across a bulk liquid membrane with substituted calyx[8]arenes and phosphoryl-containing podand

et al. 1998

View full text Add to dashboard Cite

Adamantyl-and tert-butyl-substituted calyx [8]arene esters and tripodal podand containing diphenylphosphoryl terminal groups are capable of transporting monosaccharides, but not disaccharides, across a bulk chloroform membrane. Both stoichiometry of complex formation and transport selectivity, in particular, glucose-fructose selectivity, are sensitive to the structure of a carrier.Key words: transport across a bulk liquid membrane, "host--guest" complexation; substituted calyx [S]arenes, podands; monosaccharides, disaccharides.Needs of biotechnology and pharmacology motivate interest in novel synthetic reagents for binding polyhydroxy compounds, in particular, monosaccharides and their derivatives. Synthetic membrane systems are of special interest. In our opinion, complex formation of the "host--guest" type is quite promising for creating such systems) -5 Certainly, a reagent:host" has to form firm hydrogen bonds with sugars and be lipophilic enough.The great majority of investigations in the field of transport across membranes with synthetic reagents is devoted to ionic transport, while data concerning electroneutral compounds, in particular as hydrophilic as sugars, are poorly reported. For example, transport of sugars with derivatives of boric acid has been studied, but this case does not deal with "host--guest" complexation, a Earlier, we used substituted calyx[8]arenes and podands for binding amines. 7 Those reagents are lipophilic and contain functional groups capable of forming hydrogen bonds with sugars. In the present work, we attempted to use them as carbohydrate carriers. Membrane transport was studied in a U-shaped tube (d = 19 ram). The membrane was a solution of a reagent-"host" in chloroform (V = 20 mL, C = 1 9 10 -3 mol L-l); an aqueous phase: a solution of sugar (V = 10 mL, C = 0.1 tool L -I) was used as a source solution and distilled water (V = 10 mL) was used as a receiving solution. The content of sugar in the receiving solution was controlled photometrically using a reaction with anthrone, s Results and Discussion Monosaccharides and disaccharides (D-glucose, D-d-bose, D-galactose, o-fructose, o-xylose, D-arabinose, maltose, saccharose) cannot be transported across a chloroform membrane without a carrier. The transport also was not observed in the presence of simplest lipophilic reagents capable of forming hydrogen bonds: tetradecyl alcohol and dicyclohexyl-18-crown-6 ether. Substituted calyx[8]arenes 1 and 2 transport monosaccharides (and not disaccharides) across a chloroform membrane. The selectivity of the two reagents is different (Table I). Thus, when reagent 2 is used, hexoses (fructose, galactose, and glucose), which are more hydrophilic than pentoses, are transported with a lower velocity. The transport of pentoses, in turn, substantially varies in effectiveness. Among hexoses, glucose is trans-

show abstract

Carbohydrates in Malting and Brewing Iii. Modified Method for Determining Carbohydrates by Means of the Anthrone Reagent

Hall

1956

Journal of the Institute of Brewing

View full text Add to dashboard Cite

show abstract

Factors Influencing Quantitative Determination of Methylpentoses and Ketohexoses with Anthrone

Cited by 46 publications

References 10 publications

Immunochemical Studies on the Specific Carbohydrate of Group G Streptococci

Immunochemical Studies on the Specific Carbohydrate of Group G Streptococci

Transport of carbohydrates across a bulk liquid membrane with substituted calyx[8]arenes and phosphoryl-containing podand

Carbohydrates in Malting and Brewing Iii. Modified Method for Determining Carbohydrates by Means of the Anthrone Reagent

Contact Info

Product

Resources

About