Factors influencing in vitro multiplication and rooting of peach cultivars

Hammerschlag, F. A.; Bauchan, Gary R.; Scorza, R.

doi:10.1007/bf00040950

Cited by 73 publications

(43 citation statements)

References 12 publications

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“…Antonopoulou et al (2004) reported the positive effect of a short period of darkness on the in vitro rooting. It has been reported that keeping cultures of Prunus cerasifera (Hammerschlag, 1982) and almond/peach hybrid rootstock Titan×Nemaguard (Channuntapipat et al 2003) in the dark prior to Transferring to light condition enhanced the rooting of explants. More effective photoreceptor activation is one of the factors which can be attributed to the positive effect of dark on rooting.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of GF 677 Rootstock

Aghaye¹,

Yadollahi²

2012

JAS

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The aim of this investigation was to study micropropagation of Almond GF rootstock on in vitro. Explants were cultured in MS medium containing 5 concentrations of 6-Benzylaminoporine (BAP) 0.1, 0.6, 1, 1.5 and 2 mg 1 -1 along with control treatment. Each treatment includes 5 replications. After 5 weeks of culture, the results showed that the number and length of shoots in treatment containing 1 mg 1 -1 BAP was more than the other treatments and control. The number of shoot that was obtained in treatment of 1 mg 1 -1 BAP was 2.98 (Table 1). The lowest number of shoot was 0.4 due to control treatment. For rooting, explants with 3-5 cm length were transferred to different rooting treatments ( Table 2). The results revealed that the best rooting was obtained with treatment 3 mg 1 -1 IBA. Number and length of roots were 2.167 and 11.53, respectively. Also results showed that putting explants in dark for 1 week is more effective than light.

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Section: Discussionmentioning

confidence: 99%

Micropropagation of GF 677 Rootstock

Aghaye¹,

Yadollahi²

2012

JAS

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“…Similar results obtained in species cultured in liquid or low-agar concentration media is caused by greater availability of water (Bouniols 1974;Debergh 1983) and of nutrients (Debergh 1983;Singha 1982). This increased availability may be induced by a lower resistance to diffusion and closer contact between the explant and the culture medium (Hammerschlag 1982;Romberger and Tabor 1971;Singha 1982). Although sucrose is the most commonly used energy source for cultures in most of the propagation of woody species, reducing sugars like glucose that are not normally transported in sieve tubes, can be readily taken up and metabolized providing a better carbon source (Welander et al 1989).…”

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confidence: 99%

In vitro propagation of pecan [Carya illinoinensis (Wangenh) K. Koch]

Renukdas

Manoharan

Garner

2010

Plant Biotechnology

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The pecan [Carya illinoinensis (Wangenh.) K. Koch], a member of the family Juglandaceae, is an economically important nut crop. Propagation of pecan is done primarily by budding or grafting of improved cultivars onto seedling rootstocks. However, these methods suffer disadvantages such as considerable time, expense and poor transplanting survival of the plants. Propagation by cutting, though leads to uniform clonal rootstocks, is very difficult as the clones do not root readily. Even with the use of hormones, inconsistent results with only limited improvements have been reported (Brutsch et al. 1977;Smith et al. 1974;Wolstenholme and Allan 1975). In vitro propagation offers great potential for the pecan industry for large scale multiplication of selected clones and enables production of a high amount of constant quality plantlets within a very short time. Although somatic embryogenesis has been developed for pecan (Mathews and Wetzstein 1993;Merkle et al. 1987;Wetzstein et al. 1989Wetzstein et al. , 1990Yates and Reilly 1990), successful pecan micropropagation was not achieved because of poor regeneration and rooting. Wood (1982) and Knox and Smith (1981) have used nodal stem segment explants from seedlings to establish shoot proliferation, however, no consistent shoot proliferation, elongation or root development occurred and no plants were established in soil. Phillips and Ramirez (1983) and Corte-Olivares et al. (1990) used apical and axillary buds; shoot elongation was obtained, but rooting and establishment of plantlets were poor. Hansen and Lazarte (1984) used nodal cuttings as explants; shoots were rooted with 93% success and 63% plants were established in soil while Corte-Olivares et al. (1990) obtained 40% rooting of shoots. In this article, we report the development of a reliable and efficient protocol for rapid in vitro shoot induction and rooting of pecan plants by culturing nodal explants from seed in liquid culture. Although micropropagation of pecan through nodal explants from seed will result in heterozygous plants, the protocol optimized in this study could be applied for producing true-type plants from mature tissues.The unshelled mature seeds of pecan cultivars 'Cape Fear' and 'Desirable' were washed with sterile water for 10 min and then sterilized by immersion in the carbendazim solution (1.0 g l Ϫ1; Sigma Chemical Co., St. Louis, MO, USA) overnight. The seeds were washed with sterile water and treated with 70% ethanol for 30 min., again washed with sterile water for three times to remove ethanol. Subsequently, the seeds were treated with a 2.83% (w/v) sodium hypochlorite for 30 min. The seeds were thoroughly rinsed five times with sterile water and aseptically shelled using nut cracker. The embryos were aseptically isolated from the seeds and treated with 2.83% (w/v) sodium hypochlorite solution for 10 min. and rinsed with sterilized water five times for five min Abstract An efficient method for in vitro propagation has been developed for pecan [Carya illinoinensis (Wangenh) K. Koch], a hig...

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“…Some authors have reported that the time in in vitro culture could promote somaclonal variation (Hartmann et al 1989, Nayak andSen 1991), whereas Gould (1986) has reported that culture time does not seem to be the only parameter affecting genetic stability. Genetic variation in a culture line could be affected more by a genotype than by the period in culture (Hammerschlag et al 1987, Nayak and Sen 1998, Vendrame et al 1999.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Genetic Stability of Micropropagated Curcuma caesia through Cytophotometric and Molecular Analysis

et al. 2010

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Summary Protocol has been standardized for micropropagation of medicinally important Curcuma caesia using sprouted bud explants from rhizome. Tissue culture raised plantlets of C. caesia could be conserved in vitro through subculturing at an interval of 4 months. The genetic stability of micropropagated plants was studied with an interval of 6 months up to 30 months in culture using cytophotometric, random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analysis. Cytophotometric analysis of 151 plants revealed a unimodal distribution of the DNA content with a peak corresponding to 4C nuclear DNA (3.5 pg). RAPD and ISSR analysis revealed mopnomorphic bands showing the absence of polymorphism in all 73 regenerants analyzed, thus confirming the genetic uniformity among in vitro grown somaclones of Curcuma caesia.

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Factors influencing in vitro multiplication and rooting of peach cultivars

Cited by 73 publications

References 12 publications

Micropropagation of GF 677 Rootstock

Micropropagation of GF 677 Rootstock

In vitro propagation of pecan [Carya illinoinensis (Wangenh) K. Koch]

Assessment of Genetic Stability of Micropropagated Curcuma caesia through Cytophotometric and Molecular Analysis

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