While the transportation planning literature contains many examples of the calculation of measures of accessibility for urban areas, these measures are largely restricted to motorized modes and to a handful of destination activities. This paper explores the issues related to the development of accessibility measures for non-motorized modes, namely bicycling and walking.We note that difficulties in calculating accessibility measures arise primarily from problems with data quality, the zonal structure of transportation planning models, and the adequacy of models and travel networks for describing and predicting travel by non-motorized modes. We present practical strategies for addressing these issues. The application of these methods is illustrated with the calculation of accessibility measures for a small study area in Minneapolis, MN (USA).The paper concludes with lessons learned from the process of developing non-motorized accessibility measures and ideas about their applicability to the practice of transportation planning.