Factors inducing a loss of net negative surface charge on spleen cells of mice grafted with a slow-growing tumour

Vaillier, D; Vaillier, Jacques

doi:10.1038/bjc.1980.307

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…The drop in negative charge corre sponds to factors binding to T cells and released after a 37 °C incubation [14]. Other investigations have shown that the stimulation of T cells produced a loss in negative charge [3].…”

Section: Discussionmentioning

confidence: 85%

Factors in the Sera of Tumor-Bearing Mice Modifying the Charge of Immune Cells

Vaillier¹,

Vaillier²

1983

Int Arch Allergy Immunol

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The mean electrophoretic mobility (EPM) of normal C3H spleen cells is significantly slackened (by approximately 15%) when the cells are incubated for 0.5 h at 37 °C with serum from slow-growing tumors (VMM2 and VMM1 tumors). On the contrary, it is not modified when the cells are incubated with serum from fast-growing tumors (RV2 or VFM1 tumors). VMM2 serum heated at 56°C induces the same phenomenon. Incubation at 4°C with VMM2 serum does not induce any change in spleen cell EPM. VMM2 and VMM1 sera seem to be essentially active on T cells. Indeed, no effect is observed with cells from ‘nude’, thymectomized, bone marrow-reconstituted C3H mice, or with the B cell-enriched fraction after nylon wool separation of normal spleen cells. A significant EPM decrease is only observed after serum incubation with the T cell-enriched fraction. Polyacrylamide gel electrophoresis shows that these sera exhibit an increase in the content of different protein groups. These phenomena suggest that the reduction of negative charge of lymphocytes after interaction with some tumor sera (VMM2 and VMM1 sera) might help establish tumor cell-lymphocyte contact and thus explain the slow growth rate of these VMM2 and VMM1 tumors.

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Section: Discussionmentioning

confidence: 85%

Factors in the Sera of Tumor-Bearing Mice Modifying the Charge of Immune Cells

Vaillier¹,

Vaillier²

1983

Int Arch Allergy Immunol

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“…It is known that T cell activation is ac companied by a loss of negative charge [7], The electrophoretic mobility measurement enables one to demonstrate the variations in the ratio fast/slow cells in the thymus [11] and the spleen [19] of some tumor-bearing mice, attributable in the latter cells to soluble factors modifying the cell charge. From the slow-growing VMM2 tumor, we have iso lated a mitogenic factor for B cells and a fac tor which reduces the negative surface charge of T cells and might also favor the contact tumor cells -lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

“…These tumor strains have been maintained by serial transplantations at 3-and 4-week intervals in C3H/He mice. RV2 has a higher growth rate, while VMM2 grows slowly [19]. Congenitally T lymphocytes-deficient (nu/nu) mice of Balb/c background were also used in some experiments.…”

Section: Animals and Tumorsmentioning

confidence: 99%

Stimulation and Membrane Charge Changes of Spleen Cells by Factors of Low Molecular Weight Associated with Murine Solid Tumors

Vaillier¹,

Vaillier²

1982

Pathobiology

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Dialysates from a slow-growing VMM2 and a fast-growing RV2 solid murine tumor were fractionated on a Sephadex G-50 column. Similar elution profiles with two peaks were obtained in both cases. Fractions I and II, corresponding to these two peaks were tested for their mitotic properties on lymphocytes in culture and their effect on the negative surface charge of spleen cells by the electrophoretic mobility measurement (EPM) technique. Fraction I from both tumors presents a similar mitogenic action on C3H/He or nu/nu lymphocytes in culture. Incubation for 1 h at 37 °C of normal mice spleen cells with fraction I from RV2 or VMM2 tumors did not affect the mean electrophoretic mobility of spleen cells. Fraction II from VMM2 tumor induced a significant decrease in the mean electrophoretic mobility of spleen cells (-13.4%), while fraction II from RV2 tumor increases the EPM (+ 9%) significantly. Incubation with T cell-enriched or B cell-enriched populations or spleen cells from nu/nu mice shows that fraction II from VMM2 interacts with T cells but not with B cells. Fraction II from RV2 increases the T and B cell electrophoretic mobility.

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Cytotoxicity of Subpopulations of Splenic Cells from Normal and Fibrosarcoma - Bearing Mice towards Syngeneic Tumour Cells

Aroskar

Godbole

Chaubal

1983

Tumori

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The nonadherent splenic cells from normal and tumour-bearing (mouse fibrosarcoma-MFS) Swiss mice were divided into 6 subpopulations on Percoll step density gradient and characterised. For the determination of their cytotoxicity towards syngeneic MFS cells and their electrophoretic mobility (EPM), the splenic cell populations were pooled to form 2 broad groups: a lower-density group (density of saline to just less than 1.069 g/ml) and a higher-density group (1.069 to just less than 1.087 gm/ml). In general, the splenic cells from mice bearing 10- to 11-day-old MFS tumours differed in certain characteristics from those of normal mice in that they showed an increase in the following: proliferation, heterogeneity, with appearance of large cells (greater than 70 mu2); cells with a lower density (less than 1.069 g/ml); cells with a lower (less than 0.85 micron/sec/Volt/cm) anodi cEPM. The cytotoxicity studies revealed that: a) the lower-density splenic cells of both normal and tumour-bearing mice were more cytotoxic than the higher-density splenic cells; b) the lower- and higher-density splenic cells of tumour-bearing mice were more cytotoxic than the corresponding cells of normal mice. These findings indicate that the splenic cells of mice with a lower EPM and a lower density are the main contributors of cell-mediated cytolysis of a subpopulation of MFS cells.

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Factors inducing a loss of net negative surface charge on spleen cells of mice grafted with a slow-growing tumour

Cited by 3 publications

References 14 publications

Factors in the Sera of Tumor-Bearing Mice Modifying the Charge of Immune Cells

Factors in the Sera of Tumor-Bearing Mice Modifying the Charge of Immune Cells

Stimulation and Membrane Charge Changes of Spleen Cells by Factors of Low Molecular Weight Associated with Murine Solid Tumors

Cytotoxicity of Subpopulations of Splenic Cells from Normal and Fibrosarcoma - Bearing Mice towards Syngeneic Tumour Cells

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