Factors enhancing fish sperm quality and emerging tools for sperm analysis

Effects of re-stripping on the seminal characteristics of pacu ( Piaractus mesopotamicus ) during the breeding season

“…In contrast, we observed a marked reduction in the CAGQS from the Beg period to the End period. Considering all of the evaluated parameters (Rurangwa et al, 2004;Cabrita et al, 2014), sperm with CAGQS were observed in the Beg (early spawning season, 11/17/2010 and 11/12/2013), RS1 (12/21/2010 and 12/18/2013) and RS2 (01/23/2014) groups, strongly suggesting that cellular aging may be related to decreased CAGQS. Our results suggest a decrease in pacu sperm quality throughout the spawning season and imply that using sperm from the end of the spawning period should be avoided.…”

“…The ''re-stripped'' group of Exp2 (RS1) consisted of males that were first stripped at Beg and re-stripped in Mid (n = 6), but in Exp 2, males were re-stripped for a second time (RS2) at End (n = 5) Table 1). In the previous experiment, the re-stripping procedure was effective and improved the characteristics that are associated with good-quality semen (CAGQS), as reviewed by Rurangwa et al (2004) and Cabrita et al (2014). Thus, we repeated the experiment to confirm the repeatability of re-stripping and to test the possibility of re-stripping the same male twice during the same breeding season.…”

Development of Brood-stock Diets for Nile tilapia Under Hapa-in-Pond Hatchery System; Optimal Dietary Vitamin C Level for the Optimum Reproductive Performance and Fry Survival

“…In the same context, live-bearing ornamental fish larva showed an increased survival rate in correlation with dietary ascorbic acid elevation [22]. Different doses of dietary Vitamin were suggested by many authors regarding Vitamin C form and life stage.…”

“…Meanwhile, 357 mg/kg ascorbic acid is required to obtain a 90% level of saturation and led to highest fecundity and embryo survival for rainbow trout (Oncorhynchus mykiss) [12]. The antioxidant effect of ascorbic acid seems to be critical for the genes integrity of fish gametes and decrease sperms DNA Fragmentation., meanwhile insufficient ascorbic acid lead to damage during mitotic division of germ cell [9,22]. Ascorbic acid is a leading nutrient in different tissue of reproductive system where interaction between catecholamine and steroid hormones that involved in gonad growth, acts as a regulatory mechanism of ascorbate absorption and metabolism in reproductive system [9].…”

Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium — Short communication

“…The OxyDNA test has not been used to study fish sperm quality so far (Cabrita et al, 2014). The heavy metal cadmium binds to organic matter in water and accumulates in sediments, and it can act as an Endocrine Disrupting Chemical (EDC; Gregory et al, 2008).…”