Factors Determining the Superior Performance of Lipid/DNA/Protammine Nanoparticles over Lipoplexes

Caracciolo, Giulio; Pozzi, Daniela; Capriotti, Anna Laura; Marianecci, Carlotta; Carafa, Maria; Marchini, Cristina; Montani, Maura; Amici, Augusto; Amenitsch, Heinz; Digman, Michelle A.; Gratton, Enrico; Sanchez, Susana S.; Laganà, Aldo

doi:10.1021/jm200237p

Cited by 51 publications

(41 citation statements)

References 65 publications

(135 reference statements)

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“…Whatever the involved mechanism, it is now well established that all transfection barriers are strongly affected by the ultrastructural features of DNAbased complexes. 18,19 In this study, we investigate the nanoscale structure of protamine complexes with short DNA fragments of different sizes, designed to build gene carriers in the hundreds of nanometers length-scale.…”

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confidence: 99%

Nanoscale structure of protamine/DNA complexes for gene delivery

Motta

Brocca

Favero

et al. 2013

Applied Physics Letters

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Understanding the internal packing of gene carriers is a key-factor to realize both gene protection during transport and de-complexation at the delivery site. Here, we investigate the structure of complexes formed by DNA fragments and protamine, applied in gene delivery. We found that complexes are charge- and size-tunable aggregates, depending on the protamine/DNA ratio, hundred nanometers in size. Their compactness and fractal structure depend on the length of the DNA fragments. Accordingly, on the local scale, the sites of protamine/DNA complexation assume different morphologies, seemingly displaying clumping ability for the DNA network only for shorter DNA fragments.

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confidence: 99%

Nanoscale structure of protamine/DNA complexes for gene delivery

Motta

Brocca

Favero

et al. 2013

Applied Physics Letters

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“…But to overcome this problem, researchers are lavishing many efforts to develop an efficient nanocarrier. 60 NDs can also serve as a safe and effective alternative to viral gene delivery. When coated with polyethyleneimine (PEI), a polycationic polymer, NDs are capable of safely and efficiently introducing DNA coding for the production of multiple proteins to the cells in a culture.…”

Section: Gene Therapymentioning

confidence: 99%

Biomedical Applications of Nanodiamonds: An Overview

Passeri¹,

Rinaldi²,

Ingallina³

et al. 2015

j nanosci nanotechnol

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Nanodiamonds are a novel class of nanomaterials which have raised much attention for application in biomedical field, as they combine the possibility of being produced on large scale using relatively inexpensive synthetic processes, of being fluorescent as a consequence of the presence of nitrogen vacancies, of having their surfaces functionalized, and of having good biocompatibility. Among other applications, we mainly focus on drug delivery, including cell interaction, targeting, cancer therapy, gene and protein delivery. In addition, nanodiamonds for bone and dental implants and for antibacterial use is discussed. Techniques for detection and imaging of nanodiamonds in biological tissues are also reviewed, including electron microscopy, fluorescence microscopy, Raman mapping, atomic force microscopy, thermal imaging, magnetic resonance imaging, and positron emission tomography, either in vitro, in vivo, or ex vivo. Toxicological aspects related to the use of nanodiamonds are also discussed. Finally, patents, preclinical and clinical trials based on the use of nanodiamonds for biomedical applications are reviewed.

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“…In order to study the effective electric charge of the system, electrophoretic mobility experiments were carried out by means of a laser Doppler electrophoresis technique (Malvern-NanoZetaSizer) [29]. The mobility u was converted into the zeta-potential using the Smoluchowski relation: zeta-potential = uη/ε, where ε is the permittivity of the solvent phase while η is the solvent viscosity.…”

Section: Macro and Micro-electrophoresis Experimentsmentioning

confidence: 99%

Photo-thermal effects in gold nanorods/DNA complexes

Sio

Caracciolo

Annesi

et al. 2015

Micro and Nano Syst Lett

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An ingenious combination of plasmonic nanomaterials and one of the most relevant biological systems, deoxyribonucleic acid (DNA) is achieved by bioconjugating gold nanorods (GNRs) with DNA via electrostatic interaction between positively charged GNRs and negatively charged short DNA. The obtained system is investigated as a function of DNA concentration by means of gel electrophoresis, zeta-potential, DNA melting and morphological analysis. It turns out that the obtained bioconjugated systems present both effective electric charge and aggregate size that are particularly amenable for gene therapy and nanomedicine applications. Finally, the effect of the localized (photothermal heating) and delocalized temperature variation on the DNA melting by performing both light induced bio-transparent optical heating experiments and a thermographic analysis is investigated, demonstrating that the developed system can be exploited for monitoring nanoscale temperature variation under optical illumination with very high sensitivity.

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Factors Determining the Superior Performance of Lipid/DNA/Protammine Nanoparticles over Lipoplexes

Cited by 51 publications

References 65 publications

Nanoscale structure of protamine/DNA complexes for gene delivery

Nanoscale structure of protamine/DNA complexes for gene delivery

Biomedical Applications of Nanodiamonds: An Overview

Photo-thermal effects in gold nanorods/DNA complexes

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