Factors determining self-assembly of hyaluronan

Kocourková, Karolína; Musilová, Lenka; Smolka, Petr; Mráček, Aleš; Humeník, Martin; Minařík, Antonín

doi:10.1016/j.carbpol.2020.117307

Cited by 7 publications

(4 citation statements)

References 53 publications

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“…Hence, it was crucial to take into consideration mean MWs of the HU in the modification mixture and in the treated surface. The average MWs of HU were characterized by the AF4-MALS chromatography, as described in detail in the Supporting Information of our previous work . With widely different MWs, the aggregation capacity of the system, the swelling rate, and the thickness of the swollen layer caused the coalescence of the pore-forming component ( n BT), as shown in Figure A,C.…”

Section: Resultsmentioning

confidence: 99%

“…Bacterial sodium salt of hyaluronic acid of various molecular weights was purchased from Contipro a.s. (Dolní Dobrouč, Czech Republic). The average molecular weight (MW) of HU was in the range from 330 to 1000 kDa, as characterized by the AF4-MALS chromatography in our previous work . Polystyrene (PS) Petri dishes with a diameter 34 mm, sterilized by UV radiation, free from pyrogens and DNA/RNA for cell cultivation (TPP Techno Plastic Products AG, Trasadingen, Switzerland), were used as a substrate for hyaluronan layers.…”

Section: Methodsmentioning

confidence: 99%

“…The average MWs of HU were characterized by the AF4-MALS chromatography, as described in detail in the Supporting Information of our previous work. 38 With widely different MWs, the aggregation capacity of the system, the swelling rate, and the thickness of the swollen layer caused the coalescence of the pore-forming component (nBT), as shown in Figure 9A,C. Moreover, the surface swelling rate is influenced by the residual amount of water in the HU film before the treatment, which implied tight control of the film drying before the coating procedure, as described in HU Coating Preparation.…”

Section: Texturing Of the Hu Surface By Phase Separationmentioning

confidence: 96%

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Controlled Structuring of Hyaluronan Films by Phase Separation and Inversion

Smolka,

Kadlečková,

Kocourková

et al. 2023

Langmuir

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This work explores application of phase separation phenomena for structuring of films made from hyaluronan. A timesequenced dispensing of different solution mixtures was applied under rotation of hyaluronan-covered substrates to generate surface textures. This method is applicable in direct surface modification or cover layer deposition. Changes in the surface topography were characterized by atomic force microscopy, optical microscopy, and contact and non-contact profilometry. The mechanical properties of the surface-modified self-supporting films were compared using a universal testing machine. Experimental results show that diverse hyaluronan-based surface reliefs and self-supporting films with improved mechanical properties can be prepared using a newly designed multi-step phase separation process without the need for sacrificial removable templates or additives.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Texturing Of the Hu Surface By Phase Separationmentioning

confidence: 96%

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Controlled Structuring of Hyaluronan Films by Phase Separation and Inversion

Smolka,

Kadlečková,

Kocourková

et al. 2023

Langmuir

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“…The negative charge of HA allows for non-specific adsorption on cationic surfaces such as poly (L-lysine)coated substrates and layer-by-layer approaches (Dreaden et al, 2014;Kocourková et al, 2021). Bisphosphonatefunctionalized HA was used as injectable non-covalently self-assembling hydrogel with reversible calcium binding properties (Nejadnik et al, 2014).…”

Section: Ha As Biomedical Polymer Matrixmentioning

confidence: 99%

Chemical Modification of Hyaluronan and Their Biomedical Applications

2022

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Hyaluronan, the extracellular matrix glycosaminoglycan, is an important structural component of many tissues playing a critical role in a variety of biological contexts. This makes hyaluronan, which can be biotechnologically produced in large scale, an attractive starting polymer for chemical modifications. This review provides a broad overview of different synthesis strategies used for modulating the biological as well as material properties of this polysaccharide. We discuss current advances and challenges of derivatization reactions targeting the primary and secondary hydroxyl groups or carboxylic acid groups and the N-acetyl groups after deamidation. In addition, we give examples for approaches using hyaluronan as biomedical polymer matrix and consequences of chemical modifications on the interaction of hyaluronan with cells via receptor-mediated signaling. Collectively, hyaluronan derivatives play a significant role in biomedical research and applications indicating the great promise for future innovative therapies.

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