Factors associated with adherence to and biofilm formation on polystyrene by<i>Stenotrophomonas maltophilia</i>: the role of cell surface hydrophobicity and motility

Pompilio, Arianna; Piccolomini, R; Picciani, Carla; D’Antonio, Domenico; Savini, Vincenzo; Bonaventura, Giovanni Di

doi:10.1111/j.1574-6968.2008.01292.x

Cited by 173 publications

(101 citation statements)

References 25 publications

(34 reference statements)

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“…Roosjen et al (2006) observed that the motility and zeta potential were not distinctive for adhesive and non-adhesive strains, and could therefore not be the reason for the difference in adhesion behavior. In other study, no correlation between motility, adhesion and biofilm formation was found (Pompilio et al 2008). Also, those authors found a strong relationship between the extent of initial adhesion of Stenotrophomonas maltophilia to PS surfaces and biofilm formation.…”

Section: Discussionmentioning

confidence: 90%

“…Similarly to other studies, PS was used as a model surface for adhesion and biofilm formation under laboratorial conditions (Simões et al 2007b;Pompilio et al 2008;Silva et al 2008;Johansen et al 2009). The PS microtiter plates are commonly used as the standard bioreactor system for adhesion and biofilm formation of bacteria isolated from many different environments, providing reliable comparative data (Djordjevic et al 2002;Andersson et al 2008;Cotter et al 2009).…”

Section: Discussionmentioning

confidence: 99%

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Adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria

Simões

Vieira

2010

Antonie van Leeuwenhoek

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This study was performed in order to characterize the relationship between adhesion and biofilm formation abilities of drinking water-isolated bacteria (Acinetobacter calcoaceticus, Burkholderia cepacia, Methylobacterium sp., Mycobacterium mucogenicum, Sphingomonas capsulata and Staphylococcus sp.). Adhesion was assessed by two distinct methods: thermodynamic prediction of adhesion potential by quantifying hydrophobicity and the free energy of adhesion; and by microtiter plate assays. Biofilms were developed in microtiter plates for 24, 48 and 72 h. Polystyrene (PS) was used as adhesion substratum. The tested bacteria had negative surface charge and were hydrophilic. PS had negative surface charge and was hydrophobic. The free energy of adhesion between the bacteria and PS was[ 0 mJ/m 2 (thermodynamic unfavorable adhesion). The thermodynamic approach was inappropriate for modelling adhesion of the tested drinking water bacteria, underestimating adhesion to PS. Only three (B. cepacia, Sph. capsulata and Staphylococcus sp.) of the six bacteria were non-adherent to PS. A. calcoaceticus, Methylobacterium sp. and M. mucogenicum were weakly adherent. This adhesion ability was correlated with the biofilm formation ability when comparing with the results of 24 h aged biofilms. Methylobacterium sp. and M. mucogenicum formed large biofilm amounts, regardless the biofilm age. Given time, all the bacteria formed biofilms; even those non-adherents produced large amounts of matured (72 h aged) biofilms. The overall results indicate that initial adhesion did not predict the ability of the tested drinking water-isolated bacteria to form a mature biofilm, suggesting that other events such as phenotypic and genetic switching during biofilm development and the production of extracellular polymeric substances (EPS), may play a significant role on biofilm formation and differentiation. This understanding of the relationship between adhesion and biofilm formation is important for the development of control strategies efficient in the early stages of biofilm development.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria

Simões

Vieira

2010

Antonie van Leeuwenhoek

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“…Knowing that adhesion and morphological transition are two important stages for C. albicans biofilm formation (42) and that there is a positive correlation between CSH and adhesion of C. albicans (43)(44)(45), we first examined the effect of PTE on CSH of C. albicans. The normal CSH of C. albicans was found to be 0.67 (Fig.…”

Section: Resultsmentioning

confidence: 99%

In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

Zhao

Mylonakis

et al. 2014

Antimicrob Agents Chemother

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d Pterostilbene (PTE) is a stilbene-derived phytoalexin that originates from several natural plant sources. In this study, we evaluated the activity of PTE against Candida albicans biofilms and explored the underlying mechanisms. In 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assays, biofilm biomass measurement, confocal laser scanning microscopy, and scanning electron microscopy, we found that <16 g/ml PTE had a significant effect against C. albicans biofilms in vitro, while it had no fungicidal effect on planktonic C. albicans cells, which suggested a unique antibiofilm effect of PTE. Then we found that PTE could inhibit biofilm formation and destroy the maintenance of mature biofilms. At 4 g/ ml, PTE decreased cellular surface hydrophobicity (CSH) and suppressed hyphal formation. Gene expression microarrays and real-time reverse transcription-PCR showed that exposure of C. albicans to 16 g/ml PTE altered the expression of genes that function in morphological transition, ergosterol biosynthesis, oxidoreductase activity, and cell surface and protein unfolding processes (heat shock proteins). Filamentation-related genes, especially those regulated by the Ras/cyclic AMP (cAMP) pathway, including ECE1, ALS3, HWP1, HGC1, and RAS1 itself, were downregulated upon PTE treatment, indicating that the antibiofilm effect of PTE was related to the Ras/cAMP pathway. Then, we found that the addition of exogenous cAMP reverted the PTE-induced filamentous growth defect. Finally, with a rat central venous catheter infection model, we confirmed the in vivo activity of PTE against C. albicans biofilms. Collectively, PTE had strong activities against C. albicans biofilms both in vitro and in vivo, and these activities were associated with the Ras/cAMP pathway.

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“…Since adhesion and morphological transition are two important stages for C. albicans biofilm formation (14) and there is a positive correlation between cellular surface hydrophobicity (CSH) and adhesion (15)(16)(17), we investigated the influence of SAN on CSH of C. albicans. The normal relative CSH of C. albicans was about 89.9% (Fig.…”

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confidence: 99%

Activity of Sanguinarine against Candida albicans Biofilms

Zhong

et al. 2017

Antimicrob Agents Chemother

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Candida albicans biofilms show resistance to many clinical antifungal agents and play a considerable contributing role in the process of C. albicans infections. New antifungal agents against C. albicans biofilms are sorely needed. The aim of this study was to evaluate sanguinarine (SAN) for its activity against Candida albicans biofilms and explore the underlying mechanism. The MIC 50 of SAN was 3.2 g/ ml, while Ն0.8 g/ml of SAN could suppress C. albicans biofilms. Further study revealed that Ն0.8 g/ml of SAN could decrease cellular surface hydrophobicity (CSH) and inhibited hypha formation. Real-time reverse transcription-PCR (RT-PCR) results indicated that the exposure of C. albicans to SAN suppressed the expression of some adhesion-and hypha-specific/essential genes related to the cyclic AMP (cAMP) pathway, including ALS3, HWP1, ECE1, HGC1, and CYR1. Consistently, the endogenous cAMP level of C. albicans was downregulated after SAN treatment, and the addition of cAMP rescued the SAN-induced filamentation defect. In addition, SAN showed relatively low toxicity to human umbilical vein endothelial cells, the 50% inhibitory concentration (IC 50 ) being 7.8 g/ml. Collectively, the results show that SAN exhibits strong activity against C. albicans biofilms, and the activity was associated with its inhibitory effect on adhesion and hypha formation due to cAMP pathway suppression.

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Factors associated with adherence to and biofilm formation on polystyrene byStenotrophomonas maltophilia: the role of cell surface hydrophobicity and motility

Cited by 173 publications

References 25 publications

Adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria

Adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria

In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

Activity of Sanguinarine against Candida albicans Biofilms

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