Factors and markers of virulence in from human septicemia

Abstract

One hundred Escherichia coil isolates from human septicemia were characterized ,~,,ith respect to O serogroups 1,2,4, 6,7,8, 15,18, 75 and 78, a-hemolysin, carboxylesterase B typing, cytotoxic necrotizing factor, F163 and CS31A fimbrial antigens, aerobactin production, colicins, and antibiotic sensitivity. A factorial analysis of correspondence and X-" tests indicated that most of E. coli isolates belonging to the studied O serogroups were positive for the virulence factors or markers a-haemolysin, carboxylesterase B 2 type, cytotoxic necrotizing factor, F165 fimbrial antigen and were antibiotic-sensitive (Group I). These characteris-Correspondence to:

Clonal relationships among Escherichia coli serogroup 06 isolates from human and animal infections

“…For virulence traits, we have limited our investigation to haemolysin and CNF1 production. These characteristics have already been associated with invasive E. coli [23,24]. In our study 24/26 invasive isolates were Hly and 18/26 were CNF1 positive.…”

Clonal relationships among bovine pathogenic Escherichia coli producing surface antigen CS31A

“…I. and F. Orskov according to standard techniques [15]. For a few strains, O serogroups 8, 17 and 78 were determined using an immunodot technique and also by comparison of the SDS-PAGE LPS patterns with reference O antigens as previously described [9]. SDS-PAGE analysis of OMPs was carried out on Triton X-100 insoluble membrane preparations using a 10% polyacrylamide gel [16].…”

“…A variant of CS31A was isolated from a bovine E. coli strain in the USA [8]. CS31A was also identified on E. coli strains from human septicaemia [9]. A CF 29K protein closely related to CS31A was described recently in Klebsiella strains from nosocomial infections [10].…”