Factors affectingin vitro establishment and shoot proliferation ofRosa hybrida L. andRosa Chinensis minima

Hsia, Chi Ni; Korban, Schuyler S.

doi:10.1007/bf02822690

Cited by 28 publications

(12 citation statements)

References 26 publications

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“…With the increasing distance (maturity) of node up to a certain level from apex of the shoot a gradual increase in per cent explants showing bud-break was obtained as maximum response, i.e., 78.6-81.2% bud-break took place in explants having 3rd and 4th nodes (middle order nodes), respectively (Table 2). Hsia and Korban (1996) concluded that nodal stem segments having larger diameters had a better nutrient translocation from the donor mother plant, hence developed larger buds, which sprouted sooner when placed in vitro in case of Rosa sp.…”

Section: Resultsmentioning

confidence: 98%

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

et al. 2009

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An efficient in vitro process for rapid clonal propagation of a 40-year-old tree of Azadirachta indica employing nodal stem segments was developed. Season of collection and maturity of explants showed direct influence on bud-break. Nodal stem segments collected during the month of April gave best response. Maximum bud-break (78.6-81%) was obtained when middle order nodes (3rd or 4th node from apex) were taken. Amongst different cytokinins used, 6-benzylaminopurine (BAP) at the concentration of 1.11 lM was found most effective in inducing multiple shoots, whereas inorganic and organic constituents of the medium influenced growth and general condition of proliferating shoots. On an average 3.1 shoots per explant were regenerated in modified Murashige and Skoog medium supplemented with 1.11 lM BAP, 1.43 lM indole-3-acetic acid (IAA) and 81.43 lM adenine hemisulphate. Isolated shoots were rooted in presence of 2.46 lM indole-3-butryic acid (IBA). Root induction took place in 8-10 days with 100% rooting. The in vitro-raised plantlets were successfully transplanted in potted soil and finally grown under field conditions with 100% survival. The genetic fidelity of such in vitro-raised field-grown plants was ascertained by random amplified polymorphic DNA (RAPD) markers. Furthermore, the azadirachtin content of in vitro-cloned plants was found comparable to the mother tree proving their chemical stability also. The protocol developed holds good for in vitro cloning of mature elite neem trees.

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Section: Resultsmentioning

confidence: 98%

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

et al. 2009

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“…Carefree Beauty were established using nodal stem segments collected from greenhouse-grown plants as described by Hsia and Korban (1996). Leaves from these in vitro-proliferating shoot cultures were used as explants for embryogenic callus induction as described by Li et al (2002a).…”

Section: Plant Materials and Cloning Vectormentioning

confidence: 99%

Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1 , demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa )

Gasic

Cammue

et al. 2003

Planta

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An antimicrobial protein gene, Ace-AMP1, was introduced into Rosa hybrida cv. Carefree Beauty via Agrobacterium-mediated transformation. A total of 500 putative transgenic plants were obtained from 100 primary embryogenic calli co-cultivated with A. tumefaciens following selection on a regeneration medium containing 100 mg/l kanamycin. Polymerase chain reaction analysis of these putative transgenic lines, using primers for both Ace-AMP1 and neomycin phosphotransferase ( npt II) genes, showed that 62% of these plants were positive for both transgenes. These lines were further confirmed for stable integration of Ace-AMP1 and npt II genes by Southern blotting. Transcription of the Ace-AMP1 transgene in various transgenic rose lines was determined using Northern blotting. Transgenic rose lines inoculated with conidial spores of Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. rosae showed enhanced resistance to powdery mildew using both a detached-leaf assay and an in vivo greenhouse whole-plant assay.

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“…Arora et al (2010) noticed a gradual increase in percent explants showing bud-break with the increasing distance (maturity) of node up to a certain level from apex of the shoot. Hsia and Korban (1996) concluded that nodal stem segments having larger diameters and a better nutrient translocation from the donor mother plant, hence developed larger buds, which sprouted sooner when placed in vitro in case of Rosa sp. Several species require a moderate salt concentration for their initial survival in cultures (Bhojwani and Razdan 1996;Chaturvedi et al 2004).…”

Section: Discussionmentioning

confidence: 98%

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

Singh

Chaturvedi

2010

Plant Cell Tiss Organ Cult

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A reproducible protocol for clonal propagation of Spilanthes acmella has been established. Routinely, the cultures were established in spring (January-April) season because of the highest aseptic culture establishment and high frequency shoot proliferation. Incorporation of 5 lM N 6 -benzyladenine (BA) to Murashige and Skoog (MS) basal medium showed 100% bud-break and promoted multiple shoot proliferation in cultures. Interestingly, a higher concentration of BA (7-15 lM) promoted stunted shoots with pale leaves while a lower concentration (1-3 lM) resulted in shoots with long internodes and excessive adventitious root proliferation from all over their surface. For recurrent shoot multiplication, single node segments from in vitro-developed shoots were excised and cultured on MS ? BA (5 lM) medium where 20.3-fold shoot multiplication was achieved every 5 weeks. Finally, these shoots were successfully rooted on half-strength MS medium (major salts reduced to half-strength) with 50 g l -1 sucrose, with a frequency of 100%. Transplantation survival of micropropagated plants was 88.9%. Additionally, accumulation of scopoletin, a phytoalexin, was revealed for the first time in the uninfected leaves of Spilanthes. Further, the quantitative estimation by HPLC with a fluorescence detector showed that the amounts of scopoletin content (0.10 lg g -1 DW) in the leaves of micropropagated plants are comparable to those of field-grown mother plants. The study thus signifies the effectiveness of in vitro methodology for true-to-type plant regeneration of Spilanthes and their later utility for biosynthesis and constant production of scopoletin throughout the year.

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Factors affectingin vitro establishment and shoot proliferation ofRosa hybrida L. andRosa Chinensis minima

Cited by 28 publications

References 26 publications

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1 , demonstrate enhanced resistance to powdery mildew ( Sphaerotheca pannosa )

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

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