Factors affecting tissue culture initiation from maize tassels

Rhodes, Carol A.; Green, C.E.; Phillips, Ronald L.

doi:10.1016/0168-9452(86)90196-2

Cited by 45 publications

(24 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plant regeneration has also been reported from calli initiated from different explants, namely, anthers [2][3][4], glumes [5], immature inflorescence [6], immature tassels [7][8][9], leaf segments [10][11][12], protoplasts [13], seedling segments [14][15][16], shoot tips [17][18][19], shoot apical meristem [20], mature embryos [15,21,22], and immature embryos [23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

2018

J App Biol Biotech

View full text Add to dashboard Cite

In vitro plant regeneration is preliminary requisite in the process of obtaining transgenic plants. Somatic embryogenesis is the most frequently used method of in vitro plant regeneration in maize. In this study, a reliable and reproducible maize regeneration method has been standardized using mature embryos. Inspite, the immature embryos are most suitable explants for somatic embryogenesis, obtaining these explants continuously is a laborious and tedious process, especially in summer. Hence, alternate explants of maize for in vitro regeneration have been chosen from different parts of embryonic axis. This study provides an evaluation of inbred lines and effect of hormonal combinations on total and embryogenic callus induction, their growth rate, and plantlet regeneration. Coleoptilar nodal explants from four inbred lines were cultured on different concentrations and combinations of 2,4-dichloro phenoxyacetic acid (2,4-D) along with kinetin (Kn). Explants grown on Murashige and Skoog medium supplemented with 2,4-D and Kn at a concentration of 5 and 1 mg/L, respectively, were found to be the best one for yielding a high frequency of embryogenic callus. Inbred line, NM81A was found to be the best one for in vitro culture among the four lines used. Combination of 6-benzylaminopurine and Kn at a concentration of 1 mg/L each promoted the highest frequency of shoot induction.

show abstract

Section: Introductionmentioning

confidence: 99%

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

2018

J App Biol Biotech

View full text Add to dashboard Cite

show abstract

“…Our results pointed out that a few different nuclear genes involved in the control of regeneration from wheat somatic tissue cultures, discriminate between Chinese Spring and Aquila . Nuclear genes exhibiting recessivity controlled wheat somatic embryo formation, contrary to maize, orchardgrass and alfalfa where dominance is involved (Bruneau, 1985 ;Gavin et al ., 1989 ;Hodges et al ., 1986 ;Reisch & Bingham, 1980 ;Rhodes et al ., 1986) . The fact that embryogenic capacity is a character controlled by a few genes is in agreement with other studies on genetic control in tissue culture (Hernandez & Christie, 1989) .…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of in vitro wheat somatic embryogenesis

1992

View full text Add to dashboard Cite

A spring wheat genotype which produces somatic embryos in vitro, after short and long-term culture, was tested for its ability to sexually transmit this embryogenic trait . Reciprocal crosses were performed between a embryogenic line and a nonembryogenic variety .Immature embryos were cultured on Murashige and Skoog medium plus 2 mg/12,4-dichlorophenoxyacetic acid, gelled with 5 .5 g/l agarose . Somatic embryogenesis was not expressed in the F,'s . In contrast, from several hundred immature embryos of the F 2 generation of one cross, 10 .7% and 1 .6% expressed somatic embryogenesis in short and long-term cultures respectively . These percentages of embryogenic : nonembryogenic fits a model of a few complementary genes . The embryogenic capacity of the F, genotypes depends on the presence of recessive alleles at these gene loci . The long-term wheat somatic embryogenesis capacity requires a more complex mechanism than the short-term one .

show abstract

“…Successful plant regeneration has also been reported from calli initiated from anthers (Ting et al 1981), glumes (Suprasanna et al 1986), immature inflorescences (Pareddy and Petolino 1990), immature tassels (Rhodes et al 1986;Songstad et al 1992), leaf segments (Conger et al 1987;Ray and Gosh 1990), seedling segments (Santos et al 1984), shoot tips (Zhong et al 1992;O'Connor-Sµnchez et al 2002) and shoot apical meristems (Zhang et al 2002). Immature embryo-derived callus is more efficient for plant regeneration than calli from other explant tissues.…”

Section: Introductionmentioning

confidence: 90%

High-frequency plant regeneration through callus initiation from mature embryos of maize ( Zea Mays L.)

Huang

Wei

2004

Plant Cell Reports

View full text Add to dashboard Cite

An efficient maize regeneration system was developed using mature embryos. Embryos were removed from surface-sterilized mature seeds and sliced into halves. They were used as explants to initiate callus on induction medium supplemented with 4.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). The induction frequency of primary calli was over 90% for all inbred lines tested. The primary calli were then transferred onto subculture medium supplemented with 2.0 mg l(-1) 2,4-D. Following two biweekly subcultures, embryogenic calli were formed. Inclusion of a low concentration (0.2 mg l(-1)) of 6-benzylaminopurine (BA) in the subculture medium significantly promoted the formation of embryogenic callus. The addition of silver nitrate (10 mg l(-1)) also supported an increased frequency of embryogenesis. The embryogenic callus readily formed plantlets on regeneration medium supplemented with 0.5 mg l(-1) BA. The regenerated plantlets were transferred to half-strength Murashige and Skoog medium supplemented with 0.6 mg l(-1) indole-3-butyric acid to develop healthy roots. The regenerated plantlets were successful on transfer to soil and set seed. Using this system, plantlets were regenerated from seven elite maize inbred lines. The frequency of forming green shoots ranged from 19.8% to 32.4%. This efficient regeneration system provides a solid basis for genetic transformation of maize.

show abstract

Factors affecting tissue culture initiation from maize tassels

Cited by 45 publications

References 13 publications

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

Genetic analysis of in vitro wheat somatic embryogenesis

High-frequency plant regeneration through callus initiation from mature embryos of maize ( Zea Mays L.)

Contact Info

Product

Resources

About