Factors Affecting the Variability of An in Vitro Rumen Fermentation Technique for Estimating Forage Quality

Nelson, B.D.; Ellzey, H.D.; Montgomery, C.R.; Morgan, Erin

doi:10.3168/jds.s0022-0302(72)85497-3

Cited by 26 publications

(14 citation statements)

References 14 publications

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“…Engels and van der Merwe (1967) compared lucerne hay and oaten hay inoculum sources and found a mean digestibility difference of 6 x 10"^ for a set of fourteen test feeds. Nelson et al (1972) compared five basal diets (lucerne, bahia grass, Bermuda grass, ryegrass hay and corn silage) and found a mean digestibility difference of 8 X 10"^ for this set of species prepared as test feeds. Moreover, inoculum from two of the basal rations (bahia grass, corn silage) failed to rate the test feeds in consistent order.…”

Section: Discussionmentioning

confidence: 99%

Sources of error with in vitro digestibility assay of pasture feeds

Ayres¹

1991

Grass and Forage Science

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A series of experiments was undertaken to determine population statistics for in vitro organic matter digestibility (in vitro OMD) data and to examine the effects of basal diet, donor animal and precollection fasting interval on the activity and specificity of rumen fluid inoculum. The experiments utilized wether sheep, a diverse set of pasture grass and legume feeds prominent in the Australian subtropics and the Tilley and Terry in vitro digestibility procedure running under the operating pressure of a practising feeds evaluation laboratory.The standard errors of in vitro OMD estimates for within and between batch runs were ±0-88 X 10"^ and 0-62 x 10"^, respectively. These error terms were used to develop protocols to accept, reject or scale raw in vitro OMD data. Differences between donor animals in the activity of rumen fluid were highly significant. Extending the precollection fasting interval beyond 16 h was associated with a substantial decline in inoculum activity.An in vitro-in vivo calibration relationship based on fifteen test feeds and using lucerne (Medicago sativa) as basal diet was described by the linear model y=l-3 x-0-195±4-9x 10"r =0-79 (y = in vivo OMD, x = />i vitro OMD). Despite large effects of basal diet on both the absolute values and relative ranking of test feeds, neither the RSD nor r values were improved using alternative diets to lucerne chaff.The results highlight the need to formally standardize the analytical and biological components of the in vitro digestibility procedure to safeguard the integrity of data.

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Section: Discussionmentioning

confidence: 99%

Sources of error with in vitro digestibility assay of pasture feeds

Ayres¹

1991

Grass and Forage Science

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“…Nelson et al (1972) reported that when Bermudagrass was fed to donor animals, IVDMD values for a variety of forages were higher than when perennial Ryegrass was fed; but when another warm-season grass (Bahiagrass) was fed, the IVDMD values were less than when perennial Ryegrass as fed. The laboratory needs to identify the type of samples that will be analyzed and adapt the diet of the donor animal, but no single donor diet will produce accurate results for all possible test forages (Weiss 1994).…”

Section: Methods To Estimate Digestibility Of C4 Grassesmentioning

confidence: 99%

Bahiagrass Tolerance to Aminocyclopyrachlor in Florida

et al. 2016

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Two varieties of bahiagrass were evaluated under Florida conditions for forage tolerance to the new herbicide, aminocyclopyrachlor (ACP), which is essential for product development decisions. Herbicide treatments included ACP alone at 70 and 140 g ai ha−1, ACP + chlorsulfuron at 69 + 27 and 138 + 54 g ai ha−1, ACP + 2,4-D amine at 70 + 532 g ai ha−1 and 140 + 1,064 g ai ha−1, ACP + triclopyr-amine at 70 + 140 g ai ha−1 and 140 + 280 g ai ha−1, and ACP + metsulfuron at 46 + 7, 78 + 12, and 168 + 26 g ai ha−1, and also included a nontreated check. ‘Argentine’ bahiagrass was the most tolerant forage species, and ‘Pensacola’ bahiagrass was sensitive to ACP + metsulfuron and initially to ACP + chlorsulfuron. Herbicide applications using ACP, when labeled, will likely provide good to excellent control of several weed species, with little long-term impact on bahiagrass forage production when the cultivar is known.

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“…Diversos estudos têm indicado que, no método in vitro, a digestibilidade da MS ou da fibra inicia-se na própria forragem, independentemente da fonte de inóculo ruminal (MARINUCCI et al, 1992). Entretanto, outros estudos indicam que há efeitos significativos da fonte de inóculo sobre a digestibilidade da MS (NELSON et al, 1973;GRANT et al, 1974). CHERNEY et al (1993), estudando a influência de vacas doadoras e fontes de fibra sobre a digestibilidade in vitro de MS de silagem de milho e de alfafa, com período de incubação de 48 horas, acrescentando solução de extração de NDF (fibra em detergente neutro), concluíram que a fonte de fibra na dieta de vaca doadora de líquido ruminal e o método de filtração do líquido podem afetar a digestibilidade in vitro.…”

Section: Introductionunclassified

“…NELSON et al (1973), trabalhando com a digestibilidade de cinco forragens (silagem de milho e fenos de alfafa, capim pensacola bahia, capim coastal bermuda e centeio), in situ e in vitro, encontraram diferenças significativas (P<0,05) entre digestibilidade in situ e in vitro, quando avaliaram os fenos, mas não encontraram diferenças significativos para silagem de milho.…”

Section: Introductionunclassified

Comparação do sistema de monitoramento computadorizado de digestão in vitro com os métodos in vivo e in situ: 2. Uso do resíduo da matéria seca de forragens

et al. 2000

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RESUMO -O objetivo deste trabalho foi comparar o sistema de monitoramento computadorizado da produção de gás in vitro com os métodos in vivo e in situ. Nas comparações, foram utilizadas silagens de milho com alto/baixo teores de matéria seca, com/sem inoculante. A digestibilidade das silagens com alto teor de matéria seca (MS), com/sem inoculação, não apresentou diferenças entre os métodos analisados. Quando avaliadas separadamente do efeito do inóculo, essas silagens diferiram nos métodos in vitro/gás e in situ, mas não no in vivo. Entretanto, quando analisadas somente sob efeito do inoculante, apenas no método in situ houve diferença. Não foram encontradas diferenças na digestibilidade da fibra em detergente neutro (FDN) das silagens com alta ou baixa MS, inoculadas ou não, bem como em relação ao pH ao final da digestão. Em conclusão, o desaparecimento da MS e FDN quantificada pelo resíduo no sistema in vitro/gás foi semelhante aos demais métodos avaliados. ABSTRACT -The objective of this work was to compare the computerized monitoring of the in vitro gas production system with the in vivo and in situ methods. On the comparisons, corn silage with high/low dry matter contents, with/without inoculation, were used. The digestibility of silage with high dry matter (DM) content, with/without inoculation, did not present differences among the analyzed methods. When evaluated apart from the inoculation effect, that silage differed in vitro/gas and in situ methods; however in vivo did not differ. Nevertheless, when analyzed under the inoculation effect, only in in situ method there was no difference. No differences were found on the silage neutral detergent fiber (NDF) digestibility with high or low DM, inoculated or not, as well as in relation to pH at the end of digestion. In conclusion, the disappearing of DM and NDF determined by the residue of the in vitro/gas system was similar to the others evaluated methods.Key Words: corn silage, gas production, in situ digestion, in vitro digestion, in vivo digestion Rev. bras. zootec., 29(2):531-536, 20001 Projeto financiado pela FAPESP e pelo CNPq.

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Factors Affecting the Variability of An in Vitro Rumen Fermentation Technique for Estimating Forage Quality

Cited by 26 publications

References 14 publications

Sources of error with in vitro digestibility assay of pasture feeds

Sources of error with in vitro digestibility assay of pasture feeds

Bahiagrass Tolerance to Aminocyclopyrachlor in Florida

Comparação do sistema de monitoramento computadorizado de digestão in vitro com os métodos in vivo e in situ: 2. Uso do resíduo da matéria seca de forragens

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