Factors Affecting the Transfer of Genetic Information Among Microorganisms in Soil

Stotzky, G.; Zeph, Lawrence R.; Devanas, Monica A.

doi:10.1016/b978-0-409-90199-3.50012-7

Cited by 21 publications

(11 citation statements)

References 71 publications

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“…However, the antimicrobial pressure exercised by the use of chemotherapeutic agents in fish farms as feed additives or administered directly into rearing water enhances the selection for strains resistant to these drugs, which ultimately become the predominant organisms in the bacterial population. In addition, it is known that in nature, parameters such as bacterial density and nutrient availability affect gene transfer (37). Therefore, the laboratory conditions are similar to those found in environments such as the sediments beneath fish farms that are rich in nutrients and contain large bacterial numbers (107 to 109) usually attached to particles or forming aggregates which favors contact between cells.…”

Section: Resultsmentioning

confidence: 98%

Comparison of the taxonomy, serology, drug resistance transfer, and virulence of Citrobacter freundii strains from mammals and poikilothermic hosts

Toranzo

Cutrín

Roberson

et al. 1994

Appl Environ Microbiol

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In this study, the phenotypic, antigenic, and virulence characteristics of 32 Citrobacterfreundii strains of fish, human, and veterinary origin were comparatively analyzed. In addition, the spread of drug resistance factors by conjugation was investigated. Regardless of the source of isolation, the strains exhibited variable reactions mainly for arginine dihydrolase, ornithine decarboxylase, and fermentation of sucrose, melibiose, amygdalin, and salicin. Total fatty acid methyl ester analysis by gas chromatography proved to be useful for an intratypic differentiation within the C. freundii strains studied. In fact, although all of the isolates exhibited similar fatty acid methyl ester profiles, significant differences in the major fatty acids 16:1 and 16:0 and in the 17:OA region were observed between the isolates from salmonids and the remaining strains. Serological studies using agglutination tests, analysis of lipopolysaccharides (LPS), and the corresponding immunoblots with 13 antisera indicated a great antigenic diversity among the strains. Common LPS patterns were shared only by some isolates showing high cross-agglutination titers. In contrast, although all strains exhibited very similar surface protein patterns, only two common outer membrane proteins of 54 and 58 kDa were immunologically related. Infectivity trials performed in mice and rainbow trout indicated that all of the C. freundii strains were not pathogenic for mice (50% lethal dose of >5 x 107). Although the isolates displayed a low degree of virulence

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Section: Resultsmentioning

confidence: 98%

Comparison of the taxonomy, serology, drug resistance transfer, and virulence of Citrobacter freundii strains from mammals and poikilothermic hosts

Toranzo

Cutrín

Roberson

et al. 1994

Appl Environ Microbiol

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“…These results support the hypothesis [3] that: (1) DNA binds tightly on clay minerals; (2) bound DNA is capable of transforming naturally competent cells; and (3) bound DNA is protected against degradation by bacterial nucleases. These concepts are important to microbial ecology studies, the assessment of risks associated with the introduction of genetically modified microorganisms into the environment [26,27] and to theories on the evolution of bacteria [28].…”

Section: Resultsmentioning

confidence: 99%

Transformation of Bacillus subtilis by DNA bound on clay in non-sterile soil

Gallori

Bazzicalupo

Canto

et al. 1994

FEMS Microbiology Ecology

126

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Chromosomal DNA from Bacillus subtilis and different forms of plasmid pHV14 (covalently closed circular (CCC), linear monomer (M), and linear multimer (LM)) were adsorbed and bound on the clay mineral montmorillonite. After extensive washing of the clay‐DNA complexes with DNA buffer (pH 7.5), approx. 25% of the chromosomal DNA, and approx. 30, 90, and 5%, respectively, of the CCC, M and LM form remained bound. Chromosomal and plasmid DNA bound on clay were capable of transforming competent cells, with different specific activities. The clay‐DNA complexes persisted in non‐sterile soil and retained transforming ability up to 15 days after their addition to the soil. DNA bound on montmortillonite was protected from the activity of EcoRI, supporting the evidence that DNA adsorbed on soil components was resistant to degradation by nucleases.

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“…Several physicochemical and biological factors have been shown to influence the survival of GMMs introduced into the environment [2][3][4]. Results of studies are often contradictory, especially when experiments are conducted in a heterogeneous environment such as soil.…”

Section: Introductionmentioning

confidence: 99%

Survival, plasmid transfer and impact ofpseudomonas fluorescensintroduced into soil

Kozdrój

1997

Journal of Environmental Science and Health . Part A: Environme

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The survival of donor, recipient and transconjugant strains of Pseudomonas fluorescens, transfer of plasmid RP4 from donor to recipient, and impact of the introduced strains on indigenous microflora was determined in a sandy-loam soil. The introduced donor and recipient strains survived significantly better in sterile than in non-sterile soil where a progressive decline in their numbers with time was observed. In both soils, conjugation and plasmid RP4 transfer from donor to recipient cells was observed during the first 3 days. The transconjugant survived significantly better when it was the only strain inoculated into the soil. When introduced into soil pre-colonized by the recipient strain, the transconjugant was undetectable. The introduced strains caused stimulation of total number of indigenous heterotrophic bacteria and selected physiological groups such as proteolytic, amylolytic and am-1139

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Factors Affecting the Transfer of Genetic Information Among Microorganisms in Soil

Cited by 21 publications

References 71 publications

Comparison of the taxonomy, serology, drug resistance transfer, and virulence of Citrobacter freundii strains from mammals and poikilothermic hosts

Comparison of the taxonomy, serology, drug resistance transfer, and virulence of Citrobacter freundii strains from mammals and poikilothermic hosts

Transformation of Bacillus subtilis by DNA bound on clay in non-sterile soil

Survival, plasmid transfer and impact ofpseudomonas fluorescensintroduced into soil

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