Factors affecting the shoot multiplication of Persian walnut (Juglans regia L.)

Saadat, Y A; Hennerty, M. J.

doi:10.1016/s0304-4238(02)00003-1

Cited by 25 publications

(17 citation statements)

References 16 publications

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“…For instance, the multiplication rate was 3.6 in the first subculture and it increased to 3.89 in the fourth subculture (Table II). Saadat and Hennerty [27] support these results.…”

Section: Resultsmentioning

confidence: 68%

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Micropropagation of Walnut (Juglans regia L.)

Kepenek

Kolağasi

2016

Acta Phys. Pol. A

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The objective of this work was to establish regeneration protocol for walnut (Juglans regia L.) by multiplication of shoots from axillary buds. Different concentrations of TDZ (0.0, 1.0, 2.5, 5.0 mg/l), IBA (0.0, 1.0, 2.5, 5.0 mg/l) or IBA together with TDZ were investigated to optimize regeneration for the multiplication in the NGE (Sanchez) culture medium. Multiplication rate increased with increased concentration of TDZ or IBA together with TDZ. The highest mean multiplication rate was observed in the fourth subculture with 2.5 mg/l TDZ + 2.5 mg/l IBA reaching 5.33 shoots per explant. The shoots supplemented with 5 mg/l IBA (62.71%) had higher number of rooted shoots than those supplemented with 2.5 mg/l IBA (45.66%). With 73.24% of rooting, the shoots rooted on medium containing 2.5 mg/l IBA after etiolation application had higher number of rooted shoots than those supplemented with 5 mg/l IBA with 64.69% of rooting after etiolation application. The highest mean survival rate of the shoots was observed in 2.5 mg/l TDZ + 2.5 mg/l IBA reaching 35.27%. Results showed that walnut in vitro micropropagation is feasible and the best growth percentage was obtained in 5.0 mg/l TDZ, and 2.5 mg/l TDZ with 2.5 mg/l IBA.

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“…For instance, the multiplication rate was 3.6 in the first subculture and it increased to 3.89 in the fourth subculture (Table II). Saadat and Hennerty [27] support these results.…”

Section: Resultsmentioning

confidence: 68%

“…Saadat and Hennerty obtained higher shoot proliferation both in MS media plus phytagel (2.2 g/l) and DKW media plus phytagel (2.7 new shoot per explant) [27]. The best proliferation results were obtained with 1 mg/l of BAP plus 0.01 mg/l of IBA.…”

Section: Introductionmentioning

confidence: 90%

Micropropagation of Walnut (Juglans regia L.)

Kepenek

Kolağasi

2016

Acta Phys. Pol. A

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“…Any differences observed may be a result of the higher purity of phytagel and unidentified impurities in the agar (Sharma and Ramamurthy, 2000), which could contribute to inhibiting shoot growth (Hu and Wang, 1983). There are some reports of phytagel addition improving the multiplication phase in different plants (Saadat and Hennerty, 2002;Thimmappaiah and Sadhana, 2002).…”

Section: Discussionmentioning

confidence: 99%

Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

Suárez

Pérez-Francés

Rodríguez-Pérez

2010

Span. j. agric. res.

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Leucadendron 'Safari Sunset' is one of the most valuable Proteaceae cultivar, due to its economic importance as cutflower. In vitro tissue culture of this cultivar and others may offer a massive cloned plant production that permit to satisfy the market demand. Multinodal explants of Leucadendron 'Safari Sunset' were established in vitro on a half strength Murashige and Skoog medium supplemented with 2.2 µM benzylaminopurine (BAP). Various antioxidant treatments were used to reduce the presence of phenolic compounds in culture. No important differences in growth and multiplication between these treatments were detected during in vitro establishment and multiplication, but explants cultured on a medium with 1 g L -1 of polyvinylpyrrolidone (PVP) developed a higher number of buds in the establishment phase. Proliferation of axillary shoots was achieved with 0.88 µM BAP, being slightly higher on a medium with 2.2 µM BAP although the buds were deformed, showing abnormal leaves. The use of phytagel in this phase increased proliferation rate and shoot growth. Low percentages rooting were obtained when microcuttings were cultured on the same basal medium supplemented with several indole-3-butyric acid (IBA) concentrations. The best rooting percentage (37.5%) was obtained with 44.1 µM IBA. This work shows that micropropagation of L. 'Safari Sunset' is possible with multinodal explants, although is necessary to optimize the protocol for commercial applications.Additional key words: antioxidant treatment, in vitro tissue culture, ornamental plants, phenolic compounds, Proteaceae. ResumenEmpleo de explantos multinodales para la micropropagación de Leucadendron 'Safari Sunset' Leucadendron 'Safari Sunset' es uno de los cultivares más importantes de Proteaceae debido a su importancia económica como flor cortada. El cultivo in vitro de este cultivar y otros, puede ofrecer una producción masiva de clones que permita satisfacer la demanda del mercado. Se establecieron in vitro explantos multinodales de L. 'Safari Sunset' en un medio Murashige y Skoog, con los macronutrientes a la mitad de su concentración, y bencilaminopurina (BAP) 2,2 µM. Para reducir la presencia de compuestos fenólicos en los cultivos, se emplearon diferentes tratamientos antioxidantes. No se observaron diferencias importantes en cuanto al crecimiento y multiplicación de las yemas, entre los antioxidantes empleados durante el establecimiento y multiplicación in vitro, sin embargo, se obtuvo un mayor nú-mero de yemas (25 ± 2,82) en los explantos sembrados en un medio con 1 g L -1 de polivinilpirrolidona (PVP). La multiplicación de las yemas se logró con BAP 0,88 µM. La tasa de proliferación fue ligeramente superior al adicionar al medio de cultivo BAP 2,2 µM, pero en este medio se desarrollaron yemas deformes, con hojas anormales. El empleo de fitagel incrementó la tasa de multiplicación y crecimiento de los vástagos. Se obtuvieron bajos porcentajes de enraizamiento al cultivar los microesquejes en el medio basal suplementado con diferentes concentraci...

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“…Also, the effect of genotype on rooting in different plant species were examined with several other experiments, which are compatible with present study. This shows the need of different hormonal concentrations and rooting induction period for different genotypes and also shows the effect of endogenous hormonal level on rooting (Pruski et al, 2005;Vahdati et al, 2002;Saadat and Hennerty, 2002;Rugini et al, 1993). The effect of basal MS medium supplemented with IBA found to be more effective than IAA in some cases (Al-Mizory and Mayi, 2012;James et al, 1980).…”

Section: Effect Of Indole Butyric Acid (Iba) On Rootingmentioning

confidence: 94%

“…The DKW medium known to reach to this target. Moreover, different concentrations of BAP, IBA and BA found to be stimulant to increase the walnut vegetative buds induction, propagation and rooting (Saadat and Hennerty, 2002;Driver and Kuniyuki, 1984;Penuela et al, 1988;Amiri, 2005;Jay-Allemand et al, 1992;Tetsumura et al, 2002;Sanchez-Zamora et al, 2006). In recent years, the walnut micro propagation methods are highly developed but successful shoot and root developments still in demand to commercialize the protocol.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Hormonal Treatments on Proliferation and Rooting of Three Persian Walnut (Juglans regia L.) Genotypes

Zarghami¹,

Salari²

2015

Pakistan J. of Biological Sciences

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A B S T R A C T Present study is set to develop a method to proliferate and rooting the Persian walnut (Juglans regia L.) in in vitro condition. To evaluate the proliferation of three genotypes, the DKW basal medium supplemented with four different concentrations of BAP (0, 0.5, 1 and 1.5 mg LG 1 ) and IBA (0.01 mg LG 1 ) conducted in three replications under factorial randomized block design were used. The root induction evaluated by using DKW medium supplemented by three different concentrations of IBA (0, 3 and 4 mg LG 1 ) for one week. By study of growth factors, we conclude, the BAP (1.5 mg LG 1 ) have shown appreciable results and the Chandler genotype were more responsive to the concentration compare to Hartley and Z60 genotypes. To root induction and callus initiation, IBA (3 mg LG 1 ) were found to be responsive hormone with 7 days in dark incubation. The Chandler genotype compare to the other genotypes studied here, were more responsive to this hormonal level by showing high root percentage.

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Factors affecting the shoot multiplication of Persian walnut (Juglans regia L.)

Cited by 25 publications

References 16 publications

Micropropagation of Walnut (Juglans regia L.)

Micropropagation of Walnut (Juglans regia L.)

Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

Effect of Different Hormonal Treatments on Proliferation and Rooting of Three Persian Walnut (Juglans regia L.) Genotypes

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