Factors Affecting the Quality of Cryopreserved Buffalo (<i>Bubalus bubalis</i>) Bull Spermatozoa

Andrabi, S. M. H.

doi:10.1111/j.1439-0531.2008.01240.x

Cited by 188 publications

(146 citation statements)

References 99 publications

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“…In the first case, fast thawing is suitable to prevent recrystallization of any intracellular ice which may be present in the spermatozoa (Andrabi, 2009). We found significantly higher sperm PM, RV, VAP, VSL and VCL, LIN and MMP with T3.…”

Section: Discussionmentioning

confidence: 99%

“…During cryopreservation, the spermatozoa are subjected to osmotic, thermal, and mechanical stresses, which are detectable at dilution, cooling, equilibration, freezing, and thawing stages (Andrabi, 2009). Regarding equilibration, it is traditionally called the 'glycerol equilibration' period, during which spermatozoa interact with glycerol before freezing.…”

Section: Introductionmentioning

confidence: 99%

“…In the freeze-thaw procedure, the warming phase is just as important for the survival of spermatozoa as the freezing stage (Andrabi, 2009). It is generally considered that fast rewarming rates are required for best sperm recovery.…”

Section: Introductionmentioning

confidence: 99%

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Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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SUMMARYThe effects of equilibration times (E1, 2 h; E2, 4 h; E3, 6 h), freezing rates (FR1, manual, 5 cm above liquid nitrogen (LN 2 ) for 10 min, plunging in LN 2 ; FR2, programmable ultra-fast, holding at +4°C for 2 min, from 4 to À10°C at À10°C/min, from À10 to À20°C at À15°C/min, from À20 to À120°C at À60°C/min, holding at À120°C for 30 sec, plunging in LN 2 ), and thawing rates (T1, 37°C for 30 sec; T2, 50°C for 15 sec; T3, 70°C for 7 sec) were evaluated on quality of buffalo bull spermatozoa. Progressive motility (%), rapid velocity (%), average path velocity (VAP, lm/s), straight line velocity (VSL, lm/s), and mitochondrial transmembrane potential (%) were higher (p < 0.05) with E2, FR2, and T3 compared to other groups. Sperm curved line velocity (VCL, lm/s) was higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm straightness (%) and linearity (LIN, %) were higher (p < 0.05) with E2 compared to other groups. Sperm LIN was affected (p < 0.05) with T3 compared to other groups. Supravital-plasma membrane integrity (%), viability and acrosome integrity (%) of spermatozoa were higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm DNA integrity (%) was higher (p < 0.05) with FR2 and T1 compared to other groups. We concluded that inclusion of 4 h-equilibration time, programmable ultra-fast freezing rate, and rapid thawing at 70°C for 7 sec in cryopreservation protocol improves the post-thaw quality of buffalo bull spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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“…Além de ser usado como animal de tração em diversos países, a espécie apresenta relevante papel na produção de carne e leite (LOURENÇO JÚNIOR; GARCIA, 2006), com crescente interesse pelos derivados lácteos no mercado internacional (BORGHESE;MAZZI, 2005). Para ser mais competitiva, a bubalinocultura deve elevar o padrão genético dos animais, o que pode ser facilitado pela ampliação de novos programas de inseminação artificial e consolidação dos já existentes (ANDRABI, 2009). …”

Section: Introductionunclassified

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

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This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility (P>0.05), but palm oil supplementation presented a positive effect on spermatic ability to progress (derived from BCF, STR and LIN) in Period IV (P<0.05). High correlation (91%) was observed between IP-H342 and E-N techniques (Y = 0.9155 x + 2.9843, R 2 = 0.82, P≤0.05). Thus, supplementation with UFAs increased the progressivity of spermatozoa after 90 days, and had no effect on other seminal parameters. Eosin-nigrosin stain can be used efficiently as a predictor of the level of PMI when conditions for implementing fluorescent probes not present.

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“…Many cryoprotectants like glycerol, dimethyl sulfoxide (DMSO) and sugar have been used with contrasting results in cryopreservation protocols for buffalo spermatozoa (Andrabi 2009). Among these, glycerol is the cryoprotectant of choice and is added at the concentration of 6-7 % in tris-citric acid based extender at 37°C for buffalo spermatozoa (Andrabi 2009).…”

Section: Introductionmentioning

confidence: 99%

Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa

Shah

Andrabi²,

Ahmed³

et al. 2016

Cytotechnology

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The objective of the study was to devise a cryoprotection synergism between glycerol and dimethyl sulfoxide (DMSO) for water buffalo spermatozoa. Additionally, the effect of best evolved concentrations of glycerol and DMSO in extender was assessed on in vivo fertility of buffalo spermatozoa. Ejaculates (n = 30) were equally distributed into five aliquots; first aliquot was diluted at 37°C in extender having 7 % glycerol (control); second aliquot was diluted at 37°C as well as at 4°C in extender having 3.5 % DMSO (Group 1); third aliquot was diluted at 37°C in extender having 3.5 % glycerol and then at 4°C in extender having 3.5 % DMSO (Group 2); fourth aliquot was diluted at 37°C in extender having 3.5 % DMSO and then at 4°C in extender having 3.5 % glycerol (Group 3); fifth aliquot was diluted in extenders having 1.75 % glycerol and 1.75 % DMSO at 37 as well as at 4°C (Group 4). At post thawing, sperm progressive motility (%), rapid velocity (%), average path velocity (lm/s), curved line velocity (lm/s), in vitro longevity (%), structural and functional integrity of plasmalemma (%), mitochondrial transmembrane potential (%) and viable sperm with intact acrosome (%) were higher (P \ 0.05) in Group 4 compared to other treatment groups and control. Regarding sperm DNA integrity (%); it was higher (P \ 0.05) in Group 4 compared to Group 1, 3 and control. The in vivo fertility (%) of buffalo spermatozoa was significantly higher with Group 4 compared to control (69.45 vs. 59.81). In conclusion, synergism exists between glycerol and DMSO (Group 4) in improving the quality and in vivo fertility of cryopreserved water buffalo spermatozoa.

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Factors Affecting the Quality of Cryopreserved Buffalo (Bubalus bubalis) Bull Spermatozoa

Cited by 188 publications

References 99 publications

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa

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