Factors Affecting the Production of Secondary Metabolites

Ramawat, K. G.; Mathur, M

doi:10.1201/b10756-4

Cited by 48 publications

(37 citation statements)

References 57 publications

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“…their concentration and the cytokinin/auxin ratio, on the accumulation of different groups of secondary metabolites is a well-known phenomenon (Ramawat and Mathur 2007). We documented this relationship in our earlier studies on the accumulation of other groups of secondary metabolites in in vitro cultures, e.g.…”

Section: Discussionsupporting

confidence: 56%

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Szopa

2013

Plant Growth Regul

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Phenolic acids, both benzoic and cinnamic acid derivatives, are plant metabolites with high therapeutic and cosmetic values. Methanolic extracts from the biomass of shoot and callus cultures of Aronia melanocarpa growing on seven variants of the Murashige and Skoog (MS) medium with different concentrations of plant growth regulators, BA and NAA, ranging from 0.1 to 3.0 mg l -1 , were examined for the production of free phenolic acids and cinnamic acid using the high-performance liquid chromatography (HPLC) method. The extracts from the shoot and callus cultures were confirmed to contain five of the twelve compounds tested for: caffeic, p-coumaric, p-hydroxybenzoic, syringic and vanillic acids. The shoot extracts contained additionally salicylic acid. Both the total amounts and the amounts of individual compounds in either the shoot or callus extracts were dependent on the concentration of cytokinin and auxin in the MS medium variants. The total amounts in the shoot and callus cultures were in the range from 93.52 to 217.00 mg 100 g -1 DW and from 47.11 to 83.83 mg 100 g -1 DW, respectively. The amounts of individual compounds showed wide variation, from 1.31 to 91.86 mg 100 g -1 DW in the shoot extracts, and from 2.58 to 40.16 mg 100 g -1 DW in the callus extracts. Salicylic acid (max. 91.86 mg 100 g -1 DW), p-coumaric acid (max. 62.39 mg 100 g -1 DW) and p-hydroxybenzoic acid (max. 50.66 mg 100 g -1 DW) dominated in the shoot extracts, while syringic acid (max. 40.16 mg 100 g -1 DW) and p-hydroxybenzoic acid (max. 23.59 mg 100 g -1 DW) were the main metabolites in the callus extracts. This is the first report on the quantitative analysis of benzoic and cinnamic acid derivatives in shoot and callus cultures of A. melanocarpa growing on MS-based media with different concentrations of selected plant growth regulators-BA and NAA. The obtained maximum amounts of some metabolites are of interest from a practical perspective.

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Section: Discussionsupporting

confidence: 56%

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Szopa

2013

Plant Growth Regul

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“…White light has been demonstrated to produce a beneficial effect on the accumulation of alkaloids in R. graveolens cultures and of flavonoids in Citrus aurantium cultures, as well as on the production of cardiac glycosides by Digitalis lanata or indole alkaloids and anthocyanin glycosides in Catharanthus roseus cultures (Ramawat and Mathur 2007). White light also stimulates the accumulation of betacyanins in callus cultures of Suaeda salsa (Zhao et al 2010).…”

Section: Discussionmentioning

confidence: 97%

“…The majority of studies on the effect of light quality on the production of secondary metabolites have been concerned with other groups of compounds, e.g. alkaloids, anthocyanins glycosides and flavonoids (Ramawat and Mathur 2007). Also, recently the influence of light quality on the accumulation of other groups of plant pigments such as naphthoquinones and betacyanins has been studied (Zhang et al 2010;Zhao et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Apart from light intensity and photoperiod, the quality of light is one of the known ambient factors influencing metabolite accumulation in plant in vitro cultures (Ramawat and Mathur 2007). The majority of studies on the effect of light quality on the production of secondary metabolites have been concerned with other groups of compounds, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions

Szopa

Ekiert

Szewczyk

et al. 2012

Plant Cell Tiss Organ Cult

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“…The effect of PGRs in culture media, i.e. their composition, concentration and consequently the auxin/cytokinin ratio, on the accumulation of secondary metabolites in in vitro cultures is a known phenomenon (Ramawat and Mathur 2007). It has been documented for different PGRs which influence the production of various groups of secondary metabolites, e.g.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

Szopa

Muszyńska

2012

Plant Cell Tiss Organ Cult

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Phenolic acids are plant metabolites important in phytotherapy and also in cosmetology. In this study, proliferating shoot and callus cultures of Aronia melanocarpa were established and maintained on Linsmaier and Skoog (L-S) medium containing different levels of a-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), ranging from 0.1 to 3.0 mg l -1 . Methanolic extracts from the biomass of these cultures and from the fruits of soil-grown plants were used to determine the amounts of free phenolic acids and cinnamic acid using the high-performance liquid chromatography (HPLC) method. Out of a total of twelve analyzed compounds, all of the extracts contained four of them: caffeic acid, p-hydroxybenzoic acid, syringic acid, and vanillic acid. Moreover, shoot extracts also contained salicylic acid (o-hydroxybenzoic acid), while callus extracts contained p-coumaric acid. On the other hand, fruit extracts also contained both salicylic acid and p-coumaric acid. The total amount of the analyzed compounds in extracts from both shoot and callus cultures depended on the L-S medium used, and varied between 103.05 and 150.95 mg 100 g -1 dry weight (DW), and between 50.23 and 81.56 mg 100 g -1 DW, respectively. Both types of culture contained higher levels of phenolic acids than the fruit extracts (32.43 mg 100 g -1 DW). In shoot cultures, p-hydroxybenzoic acid and salicylic acid were the predominant metabolites (reaching 55.14 and 78.25 mg 100 g -1 DW, respectively), while in callus cultures, p-hydroxybenzoic acid (25.60 mg 100 g -1 DW) and syringic acid (41.20 mg 100 g -1 DW) were the main compounds. In fruit extracts, salicylic acid (15.60 mg 100 g -1 DW) and p-hydroxybenzoic acid (5.29 mg 100 g -1 DW) were predominant.

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Factors Affecting the Production of Secondary Metabolites

Cited by 48 publications

References 57 publications

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

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