Factors affecting the primer for deoxyribonucleic acid polymerase

Inhibition of a Mammalian Deoxyribonucleic Acid Nucleotidyltransferase by Actinomycin D

“…However, no inforination has yet appeared on the action of Actinomycin D in soluble DNAsynthesizing systems derived from mammalian cells. Keir, Binnie & Smellie (1962) and Keir (1962) have described a partially purified preparation of DNA nucleotidyltransferase from Landschutz ascites-tumour cells, and in the present paper we present evidence indicating that the activity of this enzyme fraction is inhibited competitively by Actinomycin D.…”

Factors Involved in the Initiation of Multiplication of Vaccinia Virus

“…Single cycle growth studies were made and at intervals cells were disrupted by suspending in Cold Spring Harbor Laboratory Press on July 5, 2016 -Published by symposium.cshlp.org Downloaded from distilled water and briefly homogenizing in a PotterElvehjem homogenizer. The polymerase assay was based on methods described by Gray et al, 1960;Smellie, 1961;and Keir et al, 1962. DNase was assayed by estimation of added substrate DNA rendered acid soluble by incubation with the enzyme fraction under polymerase conditions, but in the absence of deoxyribonocleoside triphosphates.…”

Structure and Function of Chromatin

“…Most procedures have begun with a high-speed soluble fraction of the whole tissue, although studies by Keir et al (156) on nuclei prepared by the Behrens (16) procedure have shown the intranuclear localization of the preponderance of DNA polymerizing activity in normal cells. Most procedures have begun with a high-speed soluble fraction of the whole tissue, although studies by Keir et al (156) on nuclei prepared by the Behrens (16) procedure have shown the intranuclear localization of the preponderance of DNA polymerizing activity in normal cells.…”