Factors Affecting the Oxidative Activity of Laccase towards Biphenyl Derivatives in Homogeneous Aqueous-Organic Systems

Abstract

Catalytic oxidation of biphenyl derivatives was investigated using laccase in a homogeneous aqueous-organic system. A thermostable laccase from Trametes sp. showed the highest catalytic activity for the oxidation of 4-hydroxybiphenyl (4-HB) at a reaction temperature of 60 degrees C when dimethylsulfoxide (DMSO) was employed as a co-solvent. Furthermore, the catalytic performance was successfully enhanced by the incorporation of a laccase mediator system (LMS) into the aqueous-DMSO media. The catalytic performance strongly depended on the type of mediator, and the highest activity was observed with 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as mediator, suggesting the importance of the selection of a suitable mediator. It was verified that this mediator system is applicable to the oxidation of several biphenyl derivatives with hydroxyl groups.

Amine‐Functionalized Amino Acid‐based Ionic Liquids as Efficient and High‐Capacity Absorbents for CO2

“…As environmentally benign solvents, ionic liquids have been used in biocatalytic reactions and processes; ionic liquids more substantially enhance the technological application of enzymes than do conventional organic solvents or aqueous reaction media 176,178. The combination of unique physicochemical properties and green properties make such liquids excellent non‐aqueous solvents for enzymatic organic synthesis; they present numerous advantages, including high conversion rates, better enzyme stability, high selectivity, and better recyclability and recoverability 179…”

Amelioration of the ability to decolorize dyes by laccase: relationship between redox mediators and laccase isoenzymes in Trametes versicolor

“…However the use of mediator compounds permits an expansion of the reactivity of laccase to include such unnatural laccase substrates by generating high redox potential species. So, the use of laccase-mediator systems (LMSs) has been proposed by different authors as a promising method for bleaching pulp (Call and Mücke, 1997;Sigoillot et al 2005) and degrading xenobiotic compounds (Nagai et al 2002;Tominaga et al 2004;Dodor et al 2004), considering the increase in the oxidation capability and in the diffusibility of the generated species.…”

Applicability of Coriolopsis rigida for Biodegradation of Polycyclic Aromatic Hydrocarbons

“…Amongst the different PAHs studied, anthracene was more rapidly oxidised and selected as substrate in these experiments. Different authors propose the use of laccasemediator systems as a promising method for degrading xenobiotic compounds (Tominaga et al 2004;Dodor et al 2004). The presence of HBT led to greater anthracene oxidation than that obtained without mediator addition.…”