Factors Affecting the Gel-Permeation Chromatographic Fractionation of Low-Molecular-Weight Compounds

Multimode separation of polycyclic aromatic compounds by size exclusion chromatography with poly(divinylbenzene)

“…A reason often proposed for this observation is the fact that nonplanar PAC are cyclic compounds with structures more compact than those of the calibration compounds, so they will elute later than predicted by a MW-based calibration curve (10,(31)(32)(33)(34)(35). Nevertheless, evidence is lacking that any general 0 Chemical suppliers: (AC) Aldrich Chemical Co., Milwaukee, WI; (AN) Analabs Div., Foxboro Analytical; (CA) Caledon-American, American Bioanalytical, Natick, MA; (CO) Columbia Organic Chemical; (CS) Chem Servies, Inc., Chester, PA; (LS) Lancaster Synthesis, Inc.; (SN) Synthetic chemical.…”

“…Molecular shape can contribute to anomalous PAC retention because PAC have more compact molecular shapes than the polymeric species usually used for calibration, causing them to elute later than calibration compounds of the same molecular weight. The effect of molecular shape on Ve can be significant for small molecules and has been studied (10,(31)(32)(33)(34)(35). "Chemical suppliers: (AC) Aldrich Chemical Co., Milwaukee, WI; (CC) Cambridge Chemical Co.; (CS) Chem Services, Inc., Chester, PA. 6 [Ve], elution volume.…”

“…tron-donating and electron-withdrawing groups. Reference compounds are n-octylbenzene (17), nitrobenZene (18), toluene (19), benzene (20), bromobenzene ( 21), 2,6-di-ferf-butylnaphthalene ( 22), 1-nltronaphthalene ( 23), 2,6-dlmethylnaphthalene (24), naphthalene (25), 1 -bromonaphthalene ( 28), 1 -(bromomethyl)naphthalene ( 27), 1bromo-4-methylnaphthalene (28), 1-lodonaphthalene ( 29), 9,10-dlphenylanthracene (30), 3,6-dlmethylphenanthrene (31), 9-aminophenanthrene (32), 9-bromophenanthrene (33), 9-phenylanthracene (34), anthracene ( 35), 1,3,6,8-tetraphenylpyrene ( 38), 1-nitropyrene (37), pyrene (38), and 1-bromopyrene (39). The reference point Va…”

Densimeter detector in gel permeation chromatography of copolymers

“…(4) and (5) can be inserted into eq. (3) to yield (5) Consequently, in the GPC analysis the concentration of the solution eluting at a given retention volume is…”

New analytical techniques for mycotoxins in complex organic matrices

“…In some cases, only weak interactions are proposed [45][46][47] while other work proposes more specific mechanisms, including adsorption [48][49][50], partitioning [51], and solvent-solute hydrogen-bonding [52][53][54]. An empirical approach to correct for changes in molar volume due to the presence of various functional groups has also been employed [55].…”