1983
DOI: 10.1007/bf01386368
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Factors affecting the estimation of intracellular ethanol concentrations

Abstract: SUMMARY Estimates of intracellular ethanol concentration in batch culturesof Saooharomyoes cerevisiae were significantly affected by continued fermentation during processing of the sample. Precooling of samples to 4°C and reduction of centrifugation time significantly reduced apparent intracellular ethanol concentration.Intracellular ethanol concentrations were substantially lower than in some previous reports and fell below the extracellular ethanol concentrations during the later stages of fermentation. I NT… Show more

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Cited by 41 publications
(31 citation statements)
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References 7 publications
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“…A difference between the inhibitory effect of added and produced ethanol, in conjunction with different intracellular and extracellular ethanol concentrations, was proposed for yeast in studies prior to 1983 (34,479,497). However, subsequent unrebutted work has discounted this hypothesis for yeast (133,134,135,229,395), Z. mobilis (313), and E. coli (168). In light of these data and in particular biophysical analyses indicating that substantial differences between intracellular and extracellular ethanol concentrations across plasma membranes can exist for at most short periods (313,395), it would appear unlikely that this discrepancy is due to a difference in the inhibitory effect of added and produced ethanol.…”
Section: Native Cellulolytic Strategymentioning
confidence: 99%
“…A difference between the inhibitory effect of added and produced ethanol, in conjunction with different intracellular and extracellular ethanol concentrations, was proposed for yeast in studies prior to 1983 (34,479,497). However, subsequent unrebutted work has discounted this hypothesis for yeast (133,134,135,229,395), Z. mobilis (313), and E. coli (168). In light of these data and in particular biophysical analyses indicating that substantial differences between intracellular and extracellular ethanol concentrations across plasma membranes can exist for at most short periods (313,395), it would appear unlikely that this discrepancy is due to a difference in the inhibitory effect of added and produced ethanol.…”
Section: Native Cellulolytic Strategymentioning
confidence: 99%
“…In addition, the biotransformation product, benzyl alcohol, has been shown to directly affect membrane fluidity in studies using erythrocyte^.^^ The actual values of intracellular substrates and products must be interpreted with caution because they are dependent on the accuracy of estimates of intracellular volume and are influenced by laboratory methodology. '2,24- 26 In addition, overall intracellular levels ignore internal distribution among cell wall, membrane, and cytoplasmic phases.…”
Section: Discussionmentioning
confidence: 99%
“…Analyses Cell numbers were determined using a Coulter Counter as previously described (Dasari et al, 1983). The maximum specific growth rate during the exponential phase pm was determined from a linear regression fit of the semilog plot of cell growth.…”
Section: Methodsmentioning
confidence: 99%