Factors Affecting the Clinical Value of Microscopy for Acid-Fast Bacilli

Lipsky, Benjamin A.; Gates, Joann; Tenover, Fred C.; Plorde, James J.

doi:10.1093/clinids/6.2.214

Cited by 129 publications

(73 citation statements)

References 21 publications

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“…More patients in Group II were found with enlargement of lymph node, skin lesions and oral thrush (Table 4). Direct smear microscopy does not discriminate between tubercle bacilli and other mycobacteria, however this is not a major problem in developing world because majority of patients with AFB in sputum smears have TB as atypical mycobacteria does not present in sufficient concentration in sputum to be detected by direct microscopy (Lipsky et al, 1984). It is also possible that the direct smear microscopy may be a false positive result because of contamination by environmental bacteria or cross-contamination.…”

Section: Resultsmentioning

confidence: 99%

Improved microscopy diagnosis of pulmonary teberculosis using sodium hypochlorite concentration technique in Tanga, Tanzania

Makunde¹,

Makunde²,

Kamugisha³

et al. 2007

Tanzania J Hlth Res

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Pulmonary tuberculosis diagnosis commonly relies on the bacteriological examination of sputum. A cross-sectional hospital-based study was carried out to compare on "on the spot" sputum staining using sodium hypochlorite (bleach method) and routine Ziel-Neelsen (ZN) staining technique. Study candidates included individual patients presenting with cough <3 weeks (Group I) and ≥3 weeks (Group II). Sensitivity and specificity of the bleach method was calculated and compared at 100% using the ZN staining technique as the standard. A total of 171 patients (94 males, 77 females) with mean age 34.9 years (SD ±12.9) were recruited. Fifty-eight patients had coughed for <3 weeks while 113 had coughed for ≥3 weeks. Smear-positive TB in Group I was 13.8% (95% CI = 5-23) while in Group II was 25.7% (95% CI = 21-29). Using the bleach method, the prevalence of smear-positive TB in Group II was 28.3% . This was an increase in smear-positivity rate of 15.6% as compared to the ZN technique. These results suggest that the use of bleach technique "on the spot" improve the sensitivity of tuberculosis diagnosis among patients with a history of coughing of over three weeks. However, further studies in different settings are recommended to validate the technique. _______________________________________________________________________________________

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Section: Resultsmentioning

confidence: 99%

Improved microscopy diagnosis of pulmonary teberculosis using sodium hypochlorite concentration technique in Tanga, Tanzania

Makunde¹,

Makunde²,

Kamugisha³

et al. 2007

Tanzania J Hlth Res

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“…have a positive AFB smear of sputum or gastric aspirate (9). Fluorochrome stains such as auramine and rhodamine are superior to classical carbol fuchsin stains.…”

Section: -100mentioning

confidence: 99%

Clinical aspects of tuberculosis

Vijayan

2002

Indian J Clin Biochem

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“…Sputum examination is one of the basic and fundamental tests for diagnosing tuberculosis. Overall sensitivity of smear has been reported to range from 22-80% [2].…”

Section: Introductionmentioning

confidence: 99%