Factors affecting thawed oocyte viability suggest a customized policy of embryo transfer

Ferraretti, Anna Pia; Lappi, Michela; Magli, Maria Cristina; Muzzonigro, Francesca; Resta, Silvia; Gianaroli, Luca

doi:10.1016/j.fertnstert.2009.05.088

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…The survival rate achieved in the present study is similar to that previously observed with the 0.3 M sucrose method [7,15,16,[31][32][33][34][35][36][37] and the 0.2 M sucrose method [17,19,21]. In some of the above studies only oocytes with optimal morphology were cryopreserved [37,38] which is not the case in the present study.…”

Section: Discussionsupporting

confidence: 91%

“…Although the effect on fertilisation has been circumvented by the universal application of ICSI, similar delays have now been demonstrated to manifest in reduced implantation rates [42,43]. Although failure to obtain sperm was the main reason for oocyte cryopreservation in the present study, and would often delay the decision to cryopreserve the oocytes, we have adhered to a strict limit of cryopreservation within 5 h of oocyte retrieval.…”

Section: Discussionmentioning

confidence: 89%

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Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

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Previous reports of slow cooling of human mature oocytes have shown a reduced clinical efficiency relative to fresh oocytes. This study reports that equivalent fertilization and implantation rates to those obtained using fresh oocytes and cryopreserved embryos can be achieved with human mature oocytes dehydrated in 1.5 M propanediol and 0.2 M sucrose at 37°C and cryopreserved using slow cooling rates.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 89%

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

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“…In line with other researchers, mature oocytes which were slowly frozen within this time period yielded a higher amount of good quality embryos after ICSI than oocytes, slowly frozen beyond that time period [ 11 ]. The time at which oocyte cryopreservation is performed plays a key role in the clinical outcome of frozen-thawed oocytes and should be performed at less than 40 hours after HCG administration [ 12 ]. Cryoprotectant 1,2-propanediol and increased concentration 0.3 M sucrose were used to optimize the oocyte slow freezing procedure [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

Slow oocyte freezing and thawing in couples with no sperm or an insufficient number of sperm on the day of in vitro fertilization

Virant‐Klun

Bacer-Kermavner

Tomaževič

et al. 2011

Reprod Biol Endocrinol

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BackgroundThe clinical results of in vitro fertilization of slowly frozen-thawed oocytes are known to be significantly worse than those obtained by fresh oocytes. Little is known about the factors affecting the clinical outcome of frozen-thawed oocytes. The aim of this retrospective study was to explore the role of oocyte cryopreservation in the group of patients with no available sperm on the day of in vitro fertilization. Additionally, the effects of the female serum FSH level and sperm quality on the clinical outcome of frozen-thawed oocytes were evaluated.MethodsOocytes were slowly frozen and thawed in 22 infertile couples with no sperm or insufficient number of sperm on the day of in vitro fertilization (IVF). In 9 couples with severe azoospermia or oligoasthenoteratozoospermia frozen-thawed oocytes were fertilized by autologous sperm of bad quality when available (Group 1). In 13 couples with non-ejaculation due to psychological stress on the day of classical IVF or severe azoospermia frozen-thawed oocytes were fertilized by autologous or donated sperm of normal quality (Group 2). Oocytes were thawed in 23 cycles and microinjected by the autologous or donated sperm, when available. The clinical outcome of intracytoplasmic sperm injection - ICSI (fertilization, blastocyst, and pregnancy rates) was compared to the outcome of fresh oocytes of the same group of patients; additionally, the female serum FSH level and the sperm quality were compared.ResultsIn all couples, 70.5% of oocytes survived the freeze-thaw procedure. After ICSI, 61.5% of thawed oocytes were fertilized. Twenty one% of embryos developed to the blastocyst stage. The pregnancy rates per embryo transfer and freeze-thaw cycle were 33.3% and 17.4%, respectively. All pregnancies ended in the birth of a baby without congenital anomalies. In patients with severe azoospermia or oligoasthenoteratozoospermia there was no statistically significant difference in pregnancy rates per cycle obtained by thawed oocytes vs. fresh oocytes in previous ICSI cycles (14.2% vs. 13.6%) but there was a higher proportion of abnormal, non-cleaved or triploid zygotes when frozen-thawed oocytes were microinjected (33.3% vs. 11.8%; P < 0.01). The female serum FSH levels did not affect the survival and fertilization of frozen-thawed oocytes, but in patients with increased serum FSH level no pregnancies were achieved. After the complete freeze-thaw cycle, there was a significantly higher fertilization rate and tendency to higher pregnancy rates per thawing cycle after the microinjection of autologous or donated sperm of normal quality than autologous sperm of poor quality.ConclusionThe slow oocyte freezing and thawing is a valuable method when no or insufficient number of sperm are available on the day of in vitro fertilization. The quality of sperm is an important factor affecting the clinical outcome achieved by frozen-thawed oocytes.

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“…PCOS has also been noted to affect 28% of unselected obese and 5% of lean women (15)(16)(17)(18) . Hopefully, lifestyle intervention including dietary, exercise and behavioral therapy, stop smoking if present and leave sedentary life as can as possible, improves the symptoms and signs of this syndrome (19) . Also, there is not significant correlation between occupation of the women and their residence .…”

Section: Discussionmentioning

confidence: 99%

Analysis of Patients with PCOS According to Demographic Factors & Hormonal Assayin Babylon Government in Iraq

Mousa¹,

Joborae²

2020

MLU

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Introduction: PCO is asignificant alteration in hormonal state that happen in females which lead to ovulatory reasonsof subfertility & amenorrhea in reproductive period. Polycystic Ovarian Syndrome was collecting of presenting symptoms and signs.If we had two of the following presenting sign and symptoms,we called this condition as PCO.1. Oligomenorrhea & amenorrhea (menstrual and ovulating problem). Signs of hyperandrogenimea (clinical or biochemical).3. U/S features of polycystic ovaries which include (thick stoma on the periphery of the ovaries,which include multiple small follicles > 10and largest one < 9 mm,while the ovarian size > 10 cm3.Aims of the study :-analysis of Iraqi women in AL-Hilla city who present with problems in her menstrual cycle or fertility and assess their period of age when maximum symptoms and signs were appear,BMI, hereditary factors,occupation, residence, marital status and laboratory investigation to confirm our diagnosis (FSH, LH, AMH, PRLACTIN, TESTESTERONE) then vaginal u/s was done for married women and abdominal u/s done for unmarried one.Patients and Method: Case controlled study carried out for women that suffer from problems in her menstrual cycle and fertility from outpatient clinic in Babylon teaching hospital and some privet clinics, in period of 1year from(June 2017 to June 2018).100 complaining women were participated in this study randomly after verbal and written consent which taken from them.Results: 100 women were shared in this study, we observed that percentage of infertility increase at the current time due to PCO for unknown reason, in addition to elevation percentage of PCOS frankly according to The Rotterdam criteria(symptoms and signs of presenting women), this multisystimic disorders disturb fertility to large extent because hormonal disturbances such as increase LH\FSH ratio, Prolactin & AMH also increase ovarian volume due to thick stromal layer of the ovary then we assess affecting factors and relieving factors, its relation to patients age, BMI, occupation, residence and her hereditary state and hormonal analysis to document the diagnosis.Conclusions: Found fright increase of infertility in couples especially in our country in relative to the past, previously the incidence of infertility is about 12% of total couples and the incidence of PCO in general female about 35%(married or unmarred, may be due to delay discovery especially in unmarried group) & 45% in married women decreasing with progress of age

show abstract

Factors affecting thawed oocyte viability suggest a customized policy of embryo transfer

Cited by 8 publications

References 42 publications

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Slow oocyte freezing and thawing in couples with no sperm or an insufficient number of sperm on the day of in vitro fertilization

Analysis of Patients with PCOS According to Demographic Factors & Hormonal Assayin Babylon Government in Iraq

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