Factors affecting sperm recovery rates and survival after centrifugation of equine semen

Ferrer, Maria S.; Lyle, Sara K.; Eilts, B.E.; Eljarrah, A. H.; Paccamonti, Dale L.

doi:10.1016/j.theriogenology.2012.07.011

Cited by 14 publications

(14 citation statements)

References 18 publications

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“…Unlike pipetting, other physical stressors involved in centrifugation did not affect rat sperm motility, which leads the present authors to suggest that horse sperm will not be affected by centrifugation, as defined by Ferrer et al. (). Pipetting and centrifugation have been found to affect mitochondrial function in the rat (Kim et al., ).…”

Section: Discussionsupporting

confidence: 51%

“…Spermatozoa are exposed to physical stress from the time they are ejaculated, and their processing increases the possible cellular damage to these cells, affecting the mitochondrial function and the reactive oxygen species (ROS; Agarwal, Ikemoto, & Loughlin, 1994;Aitken, De Iuliis, Finnie, Hedges, & McLachlan, 2010;Guthrie & Welch, 2006). The most studied physical stress in spermatozoa is that provoked by centrifugation, as described by Ferrer, Lyle, Eilts, Eljarrah, and Paccamonti (2012) in equine sperm. In relation to the pipetting of sperm, Kim et al (2013) observed that rat sperm is highly sensitive to this source of stress, which accords with studies carried out by Varisli et al (2009) comparing spermatozoa from rats, mice, rams, boars and bulls.…”

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confidence: 99%

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Short‐term tolerance of equine spermatozoa to various abiotic factors

Marı́n

Arbulu

Requena

et al. 2018

Reprod Domestic Animals

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The aim of this study was to determine the effects of various abiotic factors, such as light, physical stress (pipetting) and thermal shock, on the quality of fresh and cooled equine sperm. In experiment I, four sperm aliquots were subjected to different light exposures: (i) protected control samples (CTRL), (ii) exposed to UV light at 10 cm (UV10), (iii) exposed to UV light at 20 cm (UV20) and (iv) exposed to laboratory lighting (LAB). In experiment II, four semen aliquots were subjected to repeated pipetting for 0, 10, 20 and 30 times (CTRL, P10, P20 and P30, respectively). In experiment III, four semen aliquots at 15°C were subjected to thermal oscillations: (i) cooled control sperm at 15°C (CTRL), (ii) oscillations of 1.9°C/min to a temperature of 30°C (T30), (iii) oscillations of 1.4°C/min, with the temperature rapidly falling until reaching 1.3°C (T0R) and (iv) oscillations of 1.1°C/min, with the temperature slowly falling until reaching 4.2°C (T0S). The results revealed that after 30 min, UV10 and UV20 sperm samples showed significantly (p < .05) lower total and progressive motility values, sperm kinematic parameters and mitochondrial potential. After 45 min of exposure, differences were highly significant (p < .001). No significant differences (p > .05) were found for pipetting or thermal oscillations. The results suggest that, even if equine sperm samples are not handled in the laboratory under optimal conditions, fresh and cooled equine spermatozoa are able to resist the impact of various abiotic stimuli without any reduction in their quality. This study analyses the effect on normospermic samples, but future research could look at the tolerance that asthenozoospermic equine samples have to these abiotic influences.

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Section: Discussionsupporting

confidence: 51%

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confidence: 99%

Short‐term tolerance of equine spermatozoa to various abiotic factors

Marı́n

Arbulu

Requena

et al. 2018

Reprod Domestic Animals

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“…Más aún cuando estudios donde se han evaluado bajas fuerzas de centrifugación (300 x g y 400 x g), han permitido mantener niveles de vitalidad espermática, superiores al 75% [3,20]. Sin embargo, algunos estudios indican que el aumento de la fuerza hasta 1800 x g o 2400 x g, durante un período corto de tiempo (5 min.…”

Section: Resultsunclassified

“…La centrifugación es un proceso utilizado durante el tratamiento del semen, para su almacenamiento en refrigeración y congelación [3,4]. De igual forma, se utiliza dada la necesidad de aumentar la concentración de los espermatozoides, para la inseminación artificial de las yeguas [5,6].…”

Section: El Objetivo De Este Estudio Fue Evaluar El Efecto De La Centunclassified

Efecto De La Centrifugación Sobre La Integridad Y La Funcionalidad De Espermatozoides Equinos

Betancur

Cantero-Nanclares

Páez

2016

BSAA

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“…Here, we defined the double consecutive DGC in combination with SU as DDGC/SU. It has been demonstrated that an increase in the centrifugation time or gravitational (g) force can increase the sperm recovery rate from equine semen but can also decrease sperm motility or quality due to the mechanical forces associated with centrifugation and excessive packing of the sperm 9 . Studies using spermatozoa from the epididymis of rats and mice revealed that increasing the centrifugation force decreased sperm function [10][11][12] .…”

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confidence: 99%

Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles

Dai

Wang

Cao

et al. 2020

Sci Rep

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Sperm preparation in iVf cycles using density gradient centrifugation (DGc) in combination with swim-up (SU) has been widely adopted in reproductive centres worldwide. it is a fact that the sperm recovery rate following one DGc from poor semen samples (showing liquefaction defects/containing too many unresolvable clots or rare sperm) is relatively low. our results showed that double DGc (DDGC) is effective at increasing the sperm recovery rate from poor semen samples. However, DDGC may increase the mechanical stress of sperm, thereby potentially impairing embryo development. Therefore, it is necessary to evaluate the safety of using sperm prepared by DDGC/SU for IVF cycles. In this study, we retrospectively analysed the data generated from a total of 529 IVF cycles (from June 2017 to June 2018), and these IVF cycles contributed 622 transfer cycles (from June 2017 to December 2018) in Changzhou Maternal and Child Health Care Hospital. Of them, 306 IVF cycles and the related 355 transfer cycles (normal semen samples prepared by DGC/SU) were set as the normal group, while 223 IVF cycles and the related 267 transfer cycles (poor semen prepared by DDGC/SU) were set as the observation group. The main outcome measures, including the normal fertilization rate, top D3 embryo formation rate, blastocyte formation rate, clinical pregnancy rate and live birth rate, birth weight and duration of pregnancy, were compared between the two groups. Compared to semen in the DGC/SU group, semen in the DDGC/SU group showed increased levels of the DNA fragmentation index (DFI) and reduced sperm concentration, percentage of progressive motility (PR) sperm, and percentage of normal morphology sperm. The indicators reflecting in vitro embryo development and clinical outcomes were similar in the DGC/SU group and DDGC/SU group, including the normal fertilization rate, top D3 embryo formation rate, blastocyte formation rate, pregnancy rate, implantation rate, spontaneous abortion rate, live birth rate, birth weight and duration of pregnancy. Furthermore, we found that the 1PN zygote formation rate was significantly lower in the DDGC/SU group than that in the DGC/SU group. We concluded that oocytes fertilized by sperm from poor semen samples separated by DDGC/SU achieved the same outcomes as oocytes fertilized by sperm from normal semen separated by DGC/SU, suggesting that DDGC/SU is an effective and safe method of sperm enrichment for poor semen samples in IVF. The main contribution of the present study is the verification of the effectiveness of DDGC/SU in improving sperm recovery from poor semen samples and the safety of using sperm prepared by DDGc/ SU for iVf.

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Factors affecting sperm recovery rates and survival after centrifugation of equine semen

Cited by 14 publications

References 18 publications

Short‐term tolerance of equine spermatozoa to various abiotic factors

Short‐term tolerance of equine spermatozoa to various abiotic factors

Efecto De La Centrifugación Sobre La Integridad Y La Funcionalidad De Espermatozoides Equinos

Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles

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