Factors Affecting Sperm Quality Before and After Mating of Calopterygid Damselflies

Tsuchiya, Kaori; Hayashi, Fumio

doi:10.1371/journal.pone.0009904

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…A possible explanation for the beneficial effects of EPI on the mitochondrial metabolism may be provided by nuclear magnetic resonance spectroscopy studies suggesting a high affinity of catechins for the membranous structures of spermatozoa, particularly mitochondria [46]. Consequently, proper maintenance of the mitochondrial activity, with simultaneous prevention of ROS overproduction may result in a cell that is better suited to withstand a variety of internal or external assaults [47].…”

Section: Discussionmentioning

confidence: 99%

Epicatechin Provides Antioxidant Protection to Bovine Spermatozoa Subjected to Induced Oxidative Stress

Tvrdá

Straka

Galbavy³

et al. 2019

Molecules

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Epicatechin (EPI) is a natural flavonoid with antibacterial, anti-inflammatory and anti-cancer properties. Furthermore, the molecule exhibits powerful reactive oxygen species (ROS) scavenging and metal-chelating properties. In this study, we assessed the efficiency of EPI to reverse ROS-mediated alterations to the motility, viability, DNA integrity and oxidative profile of bovine spermatozoa. For the first experiment, spermatozoa were washed out of fresh semen and exposed to 12.5 μmol/L EPI, 25 μmol/L EPI, 50 μmol/L EPI and 100 μmol/L EPI in the presence of ferrous ascorbate (FeAA) during a 6 h in vitro culture. For the second experiment, the ejaculates were split into aliquots and cryopreserved with a commercial semen extender supplemented with 12.5 μmol/L EPI, 25 μmol/L EPI, 50 μmol/L EPI, 100 μmol/L EPI or containing no supplement. Sperm motility was assessed using the computer-aided sperm analysis and the cell viability was studied with the metabolic activity test. ROS production was quantified using luminometry, and DNA fragmentation was evaluated using the chromatin dispersion test. Cell lysates were prepared at the end of the culture in order to assess the concentration of protein carbonyls and malondialdehyde. Exposure to FeAA led to a significantly reduced sperm motility (p < 0.001), mitochondrial activity (p < 0.001), but increased the generation of ROS (p < 0.001), as well as oxidative damage to proteins (p < 0.001), DNA (p < 0.001) and lipids (p < 0.001). EPI supplementation, particularly at a concentration range of 50–100 μmol/L, resulted in higher preservation of the spermatozoa vitality (p < 0.001). Furthermore, 50–100 μmol/L EPI were significantly effective in the prevention of oxidative damage to sperm proteins (p < 0.001), lipids (p < 0.001) and DNA (p < 0.01 in relation to 50 μmol/L EPI; p < 0.001 with respect to 100 μmol/L EPI). In the case of the cryopreserved spermatozoa, the administration of 50–100 μmol/L EPI resulted in higher sperm motility (p < 0.001) and mitochondrial activity (p < 0.001). ROS production, the number of protein carbonyls, lipid peroxidation as well as oxidative DNA damage were found to be significantly decreased particularly in samples cryopreserved in the presence of 100 μmol/L EPI (p < 0.001). Our results suggest that EPI could behave as an effective antioxidant which may prevent oxidative insults to spermatozoa, and thus, preserve their vitality and functionality. Nevertheless, its potential to achieve higher fertilization rates in reproductive technologies needs to be validated.

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Section: Discussionmentioning

confidence: 99%

Epicatechin Provides Antioxidant Protection to Bovine Spermatozoa Subjected to Induced Oxidative Stress

Tvrdá

Straka

Galbavy³

et al. 2019

Molecules

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“…It is usually unclear why females develop exaggerated morphologies in spermstorage organs that make sperm manipulation difficult for males. Polyandry with restricted sperm displacement may be an adaptive strategy for obtaining fresh young sperm (e.g., Lodesani et al 2004;Tsuchiya and Hayashi 2010), while minimizing the risk of an infertile mating or mating with insufficient sperm supply (Wedell et al 2000;García-González 2004;South and Lewis 2011). For female E. plebeja, infertile matings seldom occur (Kamimura 2003b).…”

Section: Alternative Hypotheses For the Function Of Elongatedmentioning

confidence: 99%

What Is Indirect Cryptic Female Choice? Theoretical Considerations and an Example from a Promiscuous Earwig

Kamimura

2015

Cryptic Female Choice in Arthropods

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Cryptic female choice (CFC) does not necessarily involve discriminative responses in the female nervous system to sperm from multiple mates. Even without any active sperm-choice mechanisms, polyandrous females can gain genetic benefits by having an arena in which genetically superior sperm are "automatically" sorted. In this chapter, possible mechanisms in this CFC category, termed "indirect CFC," are reviewed. A simple theoretical model is developed to examine the hypothesis that females obtain genetic benefits by allowing only partial displacement of stored sperm by subsequent mates. The model predicts that such restricted sperm displacement automatically grants genetic benefits when genetically superior males copulate more times per encounter with the female than less fit males. The promiscuous earwig species, Euborellia plebeja, provides an empirical example of this type of indirect CFC. The elongated female sperm-storage organs allow only partial removal and displacement of stored sperm by shorter male genitalia, resulting in a 20 % gain in paternity per mating. In staged mating trials, large males dominated male-male competition for burrows housing females, resulting in a significant increase in paternity by repeated matings with the same female. A numerical simulation based on this mating pattern showed that restricted sperm displacement (~20 % per mating) is optimal for females to accumulate sperm from larger males. Given that male body size is heritable, females were estimated to gain a 1.4 % increase in their sons' mating success as a genetic benefit. Advantages and disadvantages of indirect CFC are discussed and compared with precopulatory mate choice and direct CFC.

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“…Scathophaga stercoria (Linnaeus, 1758), Diptera (Bernasconi et al, 2002); Photinus greeni Lloyd, 1969and Photinus ignites Fall, 1927, Coleoptera (Demary, 2005; Bombus terrestris Linnaeus, 1758, Hymenoptera (Greeff & Schmid-Hempel, 2008); Mnais *Corresponding author. E-mail: francocarg@gmail.com pruinosa Selys, 1853 and Calopteryx cornelia Selys, 1853, Odonata (Tsuchiya & Hayashi, 2010)]. Moreover, the exact mechanisms of sperm viability decline have not been fully clarified, although it might be related to an intrinsic property of males (i.e.…”

Section: Introductionmentioning

confidence: 99%

Copulatory behaviour increases sperm viability in female spiders

Cargnelutti

Calbacho‐Rosa

Uñates

et al. 2020

Biological Journal of the Linnean Society

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One remarkable reproductive feature in animals with internal fertilization is a reduction in sperm viability over time in females. Whether this reduction is driven by male–male competition and/or cryptic female choice is unclear. From the perspective of cryptic female choice, we postulated that sperm viability is affected by a particular male copulatory behaviour. In this study, we investigated the following aspects: (1) sperm viability in mated females vs. males; (2) whether sperm viability varies temporally after mating; and (3) whether male copulatory behaviour covaries positively with sperm viability within females. We used the spider Holocnemus pluchei, whose males use several copulatory behaviours to court females. We found that females that stored sperm for 4 or 15 days showed no difference in sperm viability but had lower sperm viability compared with males, and males that performed a longer post-insemination behaviour had higher sperm viability inside the female. It is unclear how sperm viability is reduced and how male post-insemination behaviour affects this. It is possible that extending copulation allows males to induce females to keep sperm alive for longer. This result is predicted by theory whereby males induce females to facilitate sperm to reach and fertilize eggs based on male postcopulatory behaviour.

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Factors Affecting Sperm Quality Before and After Mating of Calopterygid Damselflies

Cited by 6 publications

References 29 publications

Epicatechin Provides Antioxidant Protection to Bovine Spermatozoa Subjected to Induced Oxidative Stress

Epicatechin Provides Antioxidant Protection to Bovine Spermatozoa Subjected to Induced Oxidative Stress

What Is Indirect Cryptic Female Choice? Theoretical Considerations and an Example from a Promiscuous Earwig

Copulatory behaviour increases sperm viability in female spiders

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