Factors Affecting Somatic Embryogenesis from Cotyledonary Explants of Safflower

Mandal, Abul Kalam Azad; Gupta, S. Dutta; Chatterji, A. K.

doi:10.1023/a:1013722116224

Cited by 23 publications

(3 citation statements)

References 19 publications

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“…Direct somatic embryogenesis in safflower is earlier reported dependent on genotype, explant age, carbon source, ethylene (Mandal et al, 2001;Mandal et al, 2002) and cytokinin and auxins. Similar results were reported for somatic embryos that are formed by embryogenic cells, which arise from somatic cells of an explant, callus or suspension cells (Gaj, 2004).…”

Section: Discussionmentioning

confidence: 95%

Indirect somatic embryogenesis from transgenic immature leaf of safflower Carthamus tinctorius (Mohler, Roth, Schmidt & Boudreaux, 1967) (Asterales: Asteraceae)

Kumar¹,

Kumari²

2017

Braz. J. Biol. Sci.

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Abstract. Highly efficient protocol is developed for indirect somatic embryogenesis from transgenic immature leaf of safflower Carthamus tinctorius (Mohler, Roth, Schmidt & Boudreaux, 1967) (Asterales: Asteraceae) explants in solid culture. Here two different half strength mediums like MSG and EMM both were tested in addition to inorganic and organic nitrogen sources and also combination of the reference medium. Inorganic nitrogen source shows about 39.5 number of embryos with 37% embryo germination and organic Nitrogen source like L-glutamine (20 μM) on reference medium influenced 80.5 number of somatic embryos with 44.9% germination. However, maximum of 92.0 number of embryo induction with 52.5% germination was noticed on CH (casein hydrolysate) (1.5 mg/L) containing medium. Combination of inorganic and organic nitrogen sources like NH 4 NO 3 (40 μM) in combinations with reduced form of organic nitrogen casein hydrolysate (CH) (1.5 mg/L) was found to be most suitable for induction of somatic embryos with a maximum of 117 number of somatic embryos with 60.9% germination in immature leaf explants. About 57% plant conversion rate showed in NARI-6 cultivar after 2 weeks of culture. However it showed maximum plant conversion rate about 68% with basal medium along with 1.5% sucrose supplemented with GA 3 (1.5 μM) and spermidine (1.0 μM). Germinated embryos with shoot and root poles were isolated and subcultured on suitable root induction medium containing NAA (2.5 μM) + AgNO 3 (1.5 μM) showed maximum rooting frequency of about 69.6%.

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Section: Discussionmentioning

confidence: 95%

Indirect somatic embryogenesis from transgenic immature leaf of safflower Carthamus tinctorius (Mohler, Roth, Schmidt & Boudreaux, 1967) (Asterales: Asteraceae)

Kumar¹,

Kumari²

2017

Braz. J. Biol. Sci.

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“…When added at low concentrations of 5 to 50 µM (0.845 to 8.45 mg/l), it was found to inhibit somatic embryo formation in Coffea canephora leaf explants (Hatanaka et al, 1995). On the other hand, media supplemented with AgNO 3 has been shown to improve somatic embryogenesis in species such as Triticum durum (Poaceae) (Fernandez et al, 1999), Coffea canephora (Rubiaceae) (Fuentes et al, (2000), Spinacia oleracea (Ishizaki et al, 2000), Carthamus tinctorius (Asteraceae) (Mandal et al, 2001), Paspalum scrobiculatum (Poaceae) (Vikrant, 2002), Bactris gasipaes (Arecaceae) (Steinmacher et al, 2007), Paspalum scrobiculatum (Poaceae) and Eleusine coracana (Poaceae) (Kothari-Chajer et al, 2008), Hedychiurn bousigonianum (Gingeberacea) (Sakhanokho et al, 2009), Gossypium nelsonii (Malvaceae), Gossypium australe (Yan et al, 2010) and Pinus taeda (Pinaceae) (Pullman et al, 2003). Kong and Yeung (1995) reported that 100 µM AgNO 3 (16.9 mg/l AgNO 3 ) stimulated embryo formation in white spruce.…”

Section: Discussionmentioning

confidence: 99%

Induction and regeneration of somatic embryos from Vitex doniana (Lamiaceae) leaf explants

Dadjo¹,

Kahia²,

Muthuri³

et al. 2015

Int. J. Biotechnol. Mol. Biol. Res.

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The present study was conducted with the aim of evaluating some of the factors that influence induction and regeneration of somatic embryos in Vitex doniana since there are no available reports on tissue culture of this tree species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog supplemented with silver nitrate and four amino acids (proline, tryptophan, lysine and leucine) at varying concentrations, 0.11 mg/l thidiazuron, 2% sucrose and 100 mg/l myo-inositol in separate experiments. The explants cultured on media supplemented with tryptophan at 30.6 mg/l produced the optimal (6.5) number of embryos per explant. This number was fivefold more than the number obtained in the control. On the other hand, it was observed that the explants on media supplemented with silver nitrate at 8.45 mg/l gave the same mean (6.5) number of embryos per explant. These first ever results on the induction of somatic embryo in V. doniana could be used for mass propagation and to select useful traits of this tree species at the cellular level. However, further work needs to be done on the conversion of the regenerated embryos.

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“…Examples of this can be seen in cotton [11], soybean [12,13], safflower [14], barley [15] and wheat [16]. In the genus Medicago, special genotypes have been produced by selection and breeding in the case of M. sativa [17] and in M. truncatula by an initial cycle of tissue culture to produce a regenerated plant which was used as a source of seed for selection over four generations [18,19].…”

Section: Selection Of the Cultivar And Type Of Explantmentioning

confidence: 99%