Factors affecting shoot organogenesis in leaf disc culture of African violet

Lo, Kuo-Chin

doi:10.1016/s0304-4238(97)00116-7

Cited by 16 publications

(11 citation statements)

References 16 publications

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“…Similar callus formation before shoot proliferation has been reported in some Gesneriaceaea species (Godo et al 2010;Takagi et al 2011). The roots that occurred on the explants were short and hairy, which corroborates the earlier reports from other Gesneriaceae species (Appelgren and Helde Ola 1972;Lo 1997;Tóth et al 2004). …”

Section: Effect Of Auxinssupporting

confidence: 90%

“…Among the members of Gesnericaeae, efficient regeneration protocols have been established in Achimenes (Vlahos et al 1995), Saintpaulia (Lo 1997), Didymocarpus (Krishnarajah et al 2002;Shaib et al 2004), Kholeria (Kozak et al 2007), Chirita (Tan et al 2009), Aeschynanthus (Cui et al 2009), Ramonda (Dontcheva et al 2009), Streptocarpus (Wei et al 2010), Metabriggsia (Ma et al 2011), Titanotrichum (Takagi et al 2011), Sinningia (Park et al 2012), Primulina (Qin et al 2013) and Lysionotus (Li et al 2013). The efficient micropropagation system calls for the genetic stability of in vitro regenerated plantlets.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India

Prameela¹,

Ramakrishnaiah²,

Krishna

et al. 2015

Physiol Mol Biol Plants

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Henckelia incana is an endemic medicinal plant used for the treatment of fever and skin allergy. In the present study shoot regeneration was evaluated on Murashige and Skoog's (MS) medium supplemented with auxins, Indole-3-acetic acid (IAA), Indole-3- butyric acid (IBA), 1-Naphthaleneacetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and cytokinins, 6-Benzylaminopurine (BAP) and Kinetin (Kn) at concentrations of 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 mgl(-1). MS medium with IBA (18.08), NAA (17.83) and IAA (17.58) at 0.5 mgl(-1) concentrations showed efficient regeneration. Regenerated shoots were rooted on half-strength MS medium with and without 0.5 mgl(-1) IBA or NAA. The plantlets were successfully hardened in rooting trays (peat, vermiculite and sand) and transferred to field mileu. The genetic fidelity of in vitro raised plants was assessed by using three different single primer amplification reaction (SPAR) markers namely random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and direct amplification of mini-satellite DNA region (DAMD). The results consistently demonstrated true-to-true type propagation. This is the first report of in vitro propagation and establishment of true-to-true type genetic fidelity in H. incana.

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Section: Effect Of Auxinssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India

Prameela¹,

Ramakrishnaiah²,

Krishna

et al. 2015

Physiol Mol Biol Plants

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“…However, Meng et al (2004) found that the abaxial leaf surface was more competent for shoot regeneration in 'Marion' blackberry. The lower shoot regeneration on the leaf surface touching the medium is possibly the result of poor gas exchange (Lo 1997). A lack of O 2 may result in inadequate supply of free energy for shoot induction and differentiation from the covered tissue (Thorpe 1988).…”

Section: Discussionmentioning

confidence: 99%

Direct shoot organogenesis and assessment of genetic stability in regenerants of Solanum aculeatissimum Jacq.

Ghimire

Chung

2011

Plant Cell Tiss Organ Cult

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A simple and efficient procedure was developed for in vitro propagation of Solanum aculeatissimum Jacq. using leaf and petiole explants cultured on Murashige and Skoog (MS) medium supplemented with a-naphthalene acetic acid (NAA) and 6-benzyladenine (BA). Effects of various plant growth regulators, explant types, carbohydrates, and basal salts on induction of adventitious shoots were also studied. Leaf explants appeared to have better regeneration capacity than petiole explants in the tested media. The highest regeneration frequency (79.33 ± 3.60%) and shoot number (11.33 ± 2.21 shoots per explant) were obtained in leaf explants in MS medium containing 3% sucrose and 0.8% agar, supplemented with 0.1 mg/l NAA and 2.0 mg/l BA, whereas petiole explants were more responsive to 0.1 mg/l NAA and 1.0 mg/l thiadiazuron. Developed shoots rooted best on MS medium with 1.0 mg/l indole acetic acid (IAA), producing 18.33 ± 2.51 roots per shoot. Histological investigation showed that the shoot buds originated mainly from epidermal cells of wounded tissues, without callus formation. The regenerated plantlets were successfully acclimatized in a greenhouse, where over 90% developed into morphologically normal and fertile plants. Results of flow cytometry analysis on S. aculeatissimum indicated no variation in the ploidy levels of plants regenerated via direct shoot formation and showed almost the same phenotype as that of mother plants. This adventitious shoot regeneration method may be used for large-scale shoot propagation and genetic engineering studies of S. aculeatissimum.

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“…Although efficient regeneration systems have already been established for several gesneriaceous species such as Streptocarpus nobilis (Handro 1983), Kohleria sp. (Geier 1988), Saintpaulia ionantha (Jungnickel and Zaid 1992;Lo 1997;Mithila et al 2003;Mølgaard et al 1991), Sinningia speciosa (Scaramuzzi et al 1999), Aeschynanthus radicans (Jin et al 2009) and Chirita spp. (Nakano et al 2009), there have been no reports of in vitro plant regeneration in T. oldhamii.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder

Takagi

Sugawara

Saito

et al. 2011

Plant Biotechnol Rep

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The gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing 0.1 mg l -1 benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing 0.1 mg l -1 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing 0.1 mg l -1 indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosomedoubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.

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Factors affecting shoot organogenesis in leaf disc culture of African violet

Cited by 16 publications

References 16 publications

Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India

Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India

Direct shoot organogenesis and assessment of genetic stability in regenerants of Solanum aculeatissimum Jacq.

Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder

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