Factors affecting plant regeneration from immature inflorescence of two winter wheat cultivars

Kavas, Musa; Öktem, Hüseyin Avni; Yücel, Meral

doi:10.1007/s10535-008-0122-4

Cited by 11 publications

(7 citation statements)

References 31 publications

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“…In Vitro PGR-induced floral reversion has been shown in a variety of dicots and monocots. In monocots, it is widely used in many commercially important crops such as grasses, palms, bananas, and grains [62,98,[100][101][102]. In dicots, floral reversion has been employed less frequently; however, it has been shown in the Brassicaceae family and has been employed in conservation efforts of recalcitrant dicots [96,97,103,104].…”

Section: Floral Reversion: An Alternate Micropropagation Approachmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

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Section: Floral Reversion: An Alternate Micropropagation Approachmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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“…A good and efficient callus induction system in wheat highly depends on sterilization process, type of explants, genotypes, media composition and its pH, growth hormones, inducers and incubation conditions (Parmar et al 2012;Mamrutha et al 2014). Different explants have been tested as starting material for wheat callus cultures, such as immature embryos (Hakam et al 2015), leaf segment (Yu et al 2012), anthers (Redha and Suleman 2011), immature inflorescence (Kavas et al 2008), microspores (Shariatpanahi et al 2006) and mature embryos (Parmar et al 2012) which showed the variable response for callus induction and regeneration. Immature embryos have been found most suitable explant source due to its high callus induction and regeneration capabilities (Redway et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Development of an efficient and reproducible regeneration system in wheat (Triticum aestivum L.)

Kumar

Mamrutha

Kaur

et al. 2017

Physiol Mol Biol Plants

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The availability of reproducible regeneration system through tissue culture is a major bottleneck in wheat improvement program. The present study has considered to develop an efficient callus induction and regeneration system using mature and immature embryos as explants in recently released agronomically superior spring wheat varieties. An efficient sterilization process was standardized using 0.1% HgCl and 70% ethanol for both seeds and embryos. The maximum possible combinations of plant growth regulators (PGRs) were evaluated for their effect on different wheat regeneration processes through tissue culture starting from callus to root induction. Picloram is found as an effective auxin with 87.63-98.67% callus induction efficiency in both explants. Supplementation of CuSO along with 2,4-D, zeatin in regeneration medium significantly enhanced the multiple shoot induction. The shoot development was achieved using full strength Murashige and Skoog's (MS) medium and root induction using half MS medium without PGRs. The optimized medium and method has resulted up to 100% regeneration irrespective of the genotype used with high reproducibility. Thus, the standardized regeneration system can be used in the regeneration of healthy plants from embryos rescued from interspecies crosses, transgenic production, induced mutation breeding and recently developed genome editing techniques for the procreation of wheat plants having novel traits.

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“…The difference may also be related to the medium used and the development stage of the inflorescences. Medium composition, callus age and vernalization period of donor plants were the main factors reported, which had great effects on inflorescences regeneration capacity (Kavas et al, 2008;Maddock et al, 1983;Pellegrineschi et al, 2003). Our preliminary studies indicate that the inflorescences which have yellowish transparent color with the length less than 2 mm cannot be induced into callus at all, and those with the sizes from 3 to 5 mm responded to induction medium, but not all of them can develop into callus, whereas those with the sizes from 5 to 8 mm can be initiated into callus easily.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that the callus induction and regeneration system is the major one used in wheat transformation, and tissue culture capacities of different explants had great effects on transformation efficiency (Pellegrineshi et al, 2002). To date, various explants such as immature embryos (Barro et al, 1999;Fennell et al, 1996;Wu et al, 2009), inflorescences (Caswell et al, 2000;Kavas et al, 2008;Maddock et al,1983), isolated anthers (Brisbe et al, 2000;Soriano et al, 2008), isolated microspores (Mejza et al, 1993;Liu et al, 2004), mature embryos Patnaik et al, 2006;Özgen et al, 1998, and shoot tips (Haliloglu et al, 2006;Viertel et al, 1996), leaf base (Wang et al, 2004) were successfully used for tissue culture, and immature embryos, 14 days after pollination with the size about 1.0 mm, were the most common explants used for transformation.…”

Section: Introductionmentioning

confidence: 99%

Tissue culture capacities of different explants from China elite common wheat and their correlation analysis

Gao¹,

FengZhi²,

Li³

et al. 2013

Afr. J. Biotechnol.

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To screen out cultivars with excellent tissue culture capacities for genetic transformation, the callus induction and regeneration response of immature embryos, inflorescences and anthers from 50 China new released cultivars with excellent agronomic performance were evaluated. The results showed that, among the surveyed cultivars, the callus induction frequency of immature embryos and inflorescences were genotype-independent. Whereas, the regeneration frequency of callus induced from immature embryos were significantly different, with less than 60%, from 60 to 80% and more than 90% in 36, eight and six cultivars, respectively. The regeneration frequency of the induced callus from inflorescences differed significantly, with less than 60%, from 40 to 90% and more than 90% in 34, 11 and five cultivars, respectively. However, both the callus induction and green plantlets regeneration frequencies of anthers were significant different among the cultivars, with the induction frequency less than 25%, from 25 to 60%, and more than 75% in 33, 14 and three cultivars, respectively. The green plantlets regeneration frequency of the callus induced from anthers ranged from 42.5 to 88.5% in different cultivars. Tissue culture capacities of immature embryos and inflorescences were highly correlated. Whereas, both of them have low correlations with anther culture capacity.

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Factors affecting plant regeneration from immature inflorescence of two winter wheat cultivars

Cited by 11 publications

References 31 publications

The Past, Present and Future of Cannabis sativa Tissue Culture

The Past, Present and Future of Cannabis sativa Tissue Culture

Development of an efficient and reproducible regeneration system in wheat (Triticum aestivum L.)

Tissue culture capacities of different explants from China elite common wheat and their correlation analysis

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