Factors Affecting Photosynthetic Activity and Stability of Isolated Mesophyll Cells from Chenopodium album

Baumann, G.; Günther, G.

doi:10.1016/s0015-3796(86)80051-8

Cited by 2 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isolation ofintact root protoplasts, modified after Baumann and Gunther (1986) 15 -20 g of freshly harvested, 5 em long root tips were chopped with a razor-blade and incubated for 20min in 250cm 3 nutrient solution with 4% sorbitol, 0.1 % BSAand 0.1 % PVP. Vacuum conditions were applied for 30s to remove the gas from the intercellular spaces and to obtain a better penetration of the medium.…”

Section: Plant Growthmentioning

confidence: 99%

ATPase Activities and Membrane Fine Structure of Rhizodermal Cells from Sorghum and Spartina Roots Grown Under Mild Salt Stress

1993

View full text Add to dashboard Cite

The study aims at correlations between ultrastructure of rhizodermal membranes and ATPase activities of two gramineous C 4-species effected by salinization of the growth medium.Comparative investigations were done with drought-resistant Sorghum and drought-sensitive, salt tolerant Spartina plants grown under steady-state conditions without or with 40 mol m -3 NaCI. Both species sustained this treatment and showed marked influences of salt on growth but not on root respiration. Generally, the intramembraneous particle (IMP) frequencies, found on freeze-fracture replicates of rhizodermal plasmic fracture faces of the plasmalemma (PPF) and tonoplast (TPF), were higher in Sorghum than in Spartina. NaCI leads in both species to an increase of the rhizodermal IMPfrequency in the TPF (-150 %) and PPF (-120 %).points out that salt-effected increases of ATPase activities of Sorghum roots were -4 times higher than the increases of IMP frequencies on rhizodermal membranes; in Spartina this effect is less intense.It is concluded that a salty environment leads to a higher ATPase activity per IMP in both species. The stronger reaction of Sorghum is explainable by the increase in K+/Na+ selectivity of the plasma membrane under salt stress. This is not the case for Spartina because the roots of this species include salt which subsequently is sequestered by the salt glands ofthe leaves. Abbreviations and SymbolsThe activities of T-and P-ATPase were determined for membrane vesicles from crude extracts and from isolated protoplasts of roots, respectively; the results from both preparations were the same. The vanadate-sensitive tissue ATPase activities increased under salt stress -5 times in Sorghum and -2 times in Spartina whilst the nitrate and azide-sensitive tissue ATPase activities increased -6 times only in Sorghum.

show abstract

Section: Plant Growthmentioning

confidence: 99%