Factors affecting in vivo viability of DNA-injected bovine blastocysts produced in vitro

Han, Yong Mahn; Lee, Chul Sang; Lee, J H; Kim, Sun Jung; Choi, Junho; Lee, H T; Chung, Byung-Hyun; Chung, Kyuha; Kim, Y H; Lee, K S; Lee, Kyung Kwang

doi:10.1016/s0093-691x(96)00235-x

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…In this context, Plachot et al (1987) proposed the natural selection of normal conceptuses against chromosome abnormalities. This fact may explain the low survival of microinjected embryos with foreign DNA Seidel 1992 andHan et al 1996). It is well known that chromosome abnormalities affect developmental potential of early embryos and may be potential predictors of developmental outcome (King et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Analysis of Transgenic Rabbit Offspring Resulting from the F4 Generations

et al. 2008

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SummaryThe present study was conducted to determine whether the chromosomal aberrations accompanied by production of transgenic rabbit could transmit through generations or abnormal cells are apparently eliminated through offspring. Two lines of transgenic and non-transgenic offspring (F4 generation), each from the same litters were produced by breeding transgenic females with non-transgenic male (line I) and transgenic females with transgenic male (line II). Altogether 5 transgenic and 3 non-transgenic rabbits from line I and 5 transgenic and 2 non-transgenic rabbits from line II, of about 9 months old were analyzed. Transgenic rabbits were produced in F0 generation by microinjection of mWAP-hFVIII gene construct into male pronucleus of fertilized eggs from superovulated New Zealand white or Californian rabbits. Samples from bone marrow cells were collected from transgenic and non-transgenic rabbits for chromosomal analysis. The results indicated that, the frequencies of chromosomal abnormalities increased significantly (pϽ0.01) in the fourth generations of transgenic rabbits expressing recombinant human clotting Factor VIII in their mammary gland. Both numerical aberrations as polyploidy and structural aberrations as fragments, deletion, centromeric attenuations and chromatid gaps were recorded. The percentage of chromosomal aberrations in transgenic F4 offspring was 2.61% for numerical and 3.37% for structural ones. Polyploidy and fragments were more frequent types of aberrations that were reported in bone marrow cells of all examined rabbits. It is concluded that, chromosomal abnormalities were transmitted to the fourth generation of transgenic rabbit offspring by low percentage. So, it will be necessary to use cytogenetic analysis for eliminating any animal with chromosomal aberrations and this will help in selecting the optimal lines for dissemination.

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Section: Discussionmentioning

confidence: 99%

Cytogenetic Analysis of Transgenic Rabbit Offspring Resulting from the F4 Generations

et al. 2008

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“…The first transgenic mice in 1982 by microinjection into introduction of foreign genes into eggs for production of transgenic animals. Especially, research on producing transgenic dairy cows that secrete large amounts of milk for the production of new biopharmaceutical proteins has been attempted by many researchers for a long time (Bowen et al, 1994;Han et al, 1996). To produce these transgenic cows, embryos at the blastocyst stage are transferred to surrogate mothers (Han et al, 1996;Richt et al, 2007;Yang et al, 2011;Xu et al, 2013;Wu et al, 2015;Su et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Especially, research on producing transgenic dairy cows that secrete large amounts of milk for the production of new biopharmaceutical proteins has been attempted by many researchers for a long time (Bowen et al, 1994;Han et al, 1996). To produce these transgenic cows, embryos at the blastocyst stage are transferred to surrogate mothers (Han et al, 1996;Richt et al, 2007;Yang et al, 2011;Xu et al, 2013;Wu et al, 2015;Su et al, 2016). The successful offspring production by fertilized embryo transfer depends on several factors on the conception rate.…”

Section: Introductionmentioning

confidence: 99%

Effect of supplement of SCM in culture medium for in vitro development of bovine in vitro fertilized oocytes

Uhm

2023

J Anim Reprod Biotechnol

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Background: The successful production of superior or transgenic offspring from in vitro produced embryos in cattle relies heavily on the quality of blastocyst stage embryos. In order to enhance the developmental competency of these embryos, a novel culture method was devised. Methods: This study utilized stem cell culture medium (SCM) from hESCs as a supplement within the culture medium for bovine in vitro produced embryos. To gauge the efficacy of this approach, in vitro fertilized embryos were subjected to culture in CR1aa medium enriched with one of three supplements: 0.3% BSA, 10% FBS, or 10% SCM. Results: The blastocyst development and hatching rates of one-cell zygotes cultured in CR1aa medium supplemented with SCM (23.9% and 10.2%) surpassed those cultured in CR1aa medium supplemented with BSA (9.3% and 0.0%) or FBS (3.1% and 0.0%) (p < 0.05). Furthermore, post-zygotic gene activation, cleaved embryos cultured in CR1aa medium supplemented with SCM (57.8% and 34.5%) exhibited notably higher rates (p < 0.05) compared to those cultured with BSA (12.9% and 0.0%) or FBS (45.7% and 22.5%) supplementation. Furthermore, the microinjection of SCM into the cytoplasm or pronucleus of fertilized zygotes resulted in elevated blastocyst development and hatching rates, particularly when the microinjected embryos were subsequently cultured in CR1aa medium supplemented with SCM from the 8-cell embryo stage onwards (p < 0.05), in contrast to those cultured with FBS supplementation. Conclusions: In conclusion, this study conclusively demonstrated that the incorporation of SCM into the culture medium significantly enhances the developmental progress of preimplantation embryos.

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Cryopreservation of in vitro-derived bovine blastocysts microinjected with foreign DNA at the pronuclear stage

Ito¹,

Otake²,

Hirabayashi³

et al. 1998

Theriogenology

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Factors affecting in vivo viability of DNA-injected bovine blastocysts produced in vitro

Cited by 6 publications

References 24 publications

Cytogenetic Analysis of Transgenic Rabbit Offspring Resulting from the F4 Generations

Cytogenetic Analysis of Transgenic Rabbit Offspring Resulting from the F4 Generations

Effect of supplement of SCM in culture medium for in vitro development of bovine in vitro fertilized oocytes

Cryopreservation of in vitro-derived bovine blastocysts microinjected with foreign DNA at the pronuclear stage

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