Factors affecting biohydrogen production by unicellular halotolerant cyanobacterium Aphanothece halophytica

Taikhao, Samart; Junyapoon, Suwannee; Incharoensakdi, Aran; Phunpruch, Saranya

doi:10.1007/s10811-012-9892-3

Cited by 37 publications

(13 citation statements)

References 56 publications

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“…In the dark, A. halophytica cells had reduced photolysis but engaged in dark respiration, leading to a lower O 2 concentration in the system and enhanced H 2 production. Moreover, nitrogen-deprived cells of A. halophytica were able to generate more H 2 from electrons acquired through the degradation of stored glycogen under dark, anaerobic conditions than from photosynthesis under light conditions [6–8].…”

Section: Discussionmentioning

confidence: 99%

“…H 2 concentration in 500 μ L of headspace gas was analyzed by gas chromatograph (Hewlett-Packard HP5890A, Japan) with a molecular sieve 5°A, 60/80 mesh packed column using a thermal conductivity detector under previously described conditions [6]. The H 2 production rate was calculated as a term of μ mol H 2 g −1 dry weight h −1 .…”

Section: Methodsmentioning

confidence: 99%

“…The unicellular cyanobacterium Aphanothece halophytica is a halotolerant microorganism that can grow in a wide range of salinity from 0.25 to 3.0 M NaCl [5]. A. halophytica produces a large amount of dark fermentative H 2 compared with other marine cyanobacteria [6, 7]. H 2 production by A. halophytica is catalyzed by bidirectional hydrogenase and occurs particularly under nitrogen-deprived and dark anaerobic conditions [6–8].…”

Section: Introductionmentioning

confidence: 99%

“…A. halophytica produces a large amount of dark fermentative H 2 compared with other marine cyanobacteria [6, 7]. H 2 production by A. halophytica is catalyzed by bidirectional hydrogenase and occurs particularly under nitrogen-deprived and dark anaerobic conditions [6–8]. Hydrogenase is the only enzyme that catalyzes both H 2 uptake and H 2 production in this organism [8].…”

Section: Introductionmentioning

confidence: 99%

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Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Pansook

Incharoensakdi

Phunpruch

2019

The Scientific World Journal

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The unicellular halotolerant cyanobacterium Aphanothece halophytica is a potential dark fermentative producer of molecular hydrogen (H2) that produces very little H2 under illumination. One factor limiting the H2 photoproduction of this cyanobacterium is an inhibition of bidirectional hydrogenase activity by oxygen (O2) obtained from splitting water molecules via photosystem II activity. The present study aimed to investigate the effects of the photosystem II inhibitors carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) on H2 production of A. halophytica under light and dark conditions and on photosynthetic and respiratory activities. The results showed that A. halophytica treated with CCCP and DCMU produced H2 at three to five times the rate of untreated cells, when exposed to light. The highest H2 photoproduction rates, 2.26 ± 0.24 and 3.63 ± 0.26 μmol H2 g−1 dry weight h−1, were found in cells treated with 0.5 μM CCCP and 50 μM DCMU, respectively. Without inhibitor treatment, A. halophytica incubated in the dark showed a significant increase in H2 production compared with cells that were incubated in the light. Only CCCP treatment increased H2 production of A. halophytica during dark incubation, because CCCP functions as an uncoupling agent of oxidative phosphorylation. The highest dark fermentative H2 production rate of 39.50 ± 2.13 μmol H2 g−1 dry weight h−1 was found in cells treated with 0.5 μM CCCP after 2 h of dark incubation. Under illumination, CCCP and DCMU inhibited chlorophyll fluorescence, resulting in a low level of O2, which promoted bidirectional hydrogenase activity in A. halophytica cells. In addition, only CCCP enhanced the respiration rate, further reducing the O2 level. In contrast, DCMU reduced the respiration rate in A. halophytica.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Pansook

Incharoensakdi

Phunpruch

2019

The Scientific World Journal

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“…Hydrogen-evolving properties of halotolerant cyanobacteria are also being explored; of those, Aphanothece halophytica demonstrates a good potential with hydrogen production rate up to 14 μmol/(mg chlorophyll × h) [37].…”

Section: Cyanobacterial Nitrogenasementioning

confidence: 99%

Advances in Utilizing Cyanobacteria for Hydrogen Production

Kufryk¹

2013

AiM

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Cyanobacteria are photoautotrophic prokaryotes with a remarkable metabolic flexibility. Many species of cyanobacteria produce hydrogen, and the efficiency of this process can be improved by genetic engineering. Isolated photosynthetic complexes of cyanobacteria that are capable of light absorption and charge separation can be utilized in hydrogen-producing devices that are driven by solar energy. As photosynthetic microorganisms, cyanobacteria present a unique opportunity for creating low cost systems for hydrogen production in vivo and in vitro. This review is focused on recent advances in cyanobacterial hydrogen research.

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Advances in Whole‐Cell Photobiological Hydrogen Production

Chen

Wang

et al. 2021

Advanced NanoBiomed Research

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Solar energy is the largest energy source on Earth. In contrast to the limited and greenhouse gases‐emitting fossil fuels, solar energy is inexhaustible, carbon neutral, and nonpolluting. The conversion of this most abundant but highly diffused source into hydrogen is increasingly attractive. In nature, photosynthetic microorganisms exploit solar energy to produce hydrogen via photosynthesis, which is also known as photobiological hydrogen production. More recently, various types of artificial materials have been developed to hybrid microorganisms for converting solar energy into hydrogen, namely, semiartificial photosynthesis hydrogen production. Herein, the strategies for converting solar energy into hydrogen with whole‐cell biocatalyst are summarized and their potentials for future social sustainable development are discussed.

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Factors affecting biohydrogen production by unicellular halotolerant cyanobacterium Aphanothece halophytica

Cited by 37 publications

References 56 publications

Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Advances in Utilizing Cyanobacteria for Hydrogen Production

Advances in Whole‐Cell Photobiological Hydrogen Production

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