Factors affecting adventitious root formation in microcuttings of Malus

Klerk, GJ De; Brugge, J. Ter

doi:10.1051/agro:19921002

Cited by 20 publications

(3 citation statements)

References 25 publications

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“…This demonstrates that the process of root formation on stem slices is highly susceptible for kanamycin especially during the first two days. The timing coincides well with the root induction phase and the initial differentiation events, such as the first cell divisions, as described for apple [2] .…”

Section: Discussionsupporting

confidence: 78%

The effects of exogenous auxin and rol genes on root formation in Rosa hybrida L. ‘Moneyway’

Salm¹,

Toorn²,

Cate³

et al. 1996

Plant Growth Regul

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The effect of indole-3-butyric acid (IBA) on the formation of non-transformed and rol gene transformed roots on stem slices of in vitro cultured shoots of Rosa hybridu L. 'Moneyway' was examined. Formation of adventitious roots on this rootstock was dependent on the IBA dose; it was not affected by the presence of other root primordia on the same explant. Application of 0.32 to 1 PM IBA during 5 days, followed by transfer to medium without hormones resulted in maximum root formation (90%) after three weeks. The formation of such untransformed roots was completely inhibited by transfer to medium with 5 mg 1-l kanamycin two days after excision. Ri roots were formed upon inoculation with A. rhizogenes LBA9402 harbouring two plasmids: pRil855, comprising the rol genes and the binary plasmid p 35Sgusintron with the nptll gene for kanamycin resistance and the CaMV 35Sgusintron gene. The formation of these Ri roots on kanamycin-containing medium was independent of the presence of IBA. Stem slices inoculated with a disarmed A. tumefuciens GV3 101, harbouring only the nptll gene, formed callus and subsequently roots in the presence of kanamycin exclusively on medium with high IBA concentrations (10 or 100 pw. Root formation at 100 PM IBA was considerably improved by transformation with the rolB gene under the influence of the strong CaMV 35s promoter. In addition, low IBA (0.1 and 1 ,uM) stimulated the formation of roots only on stem slices transformed with A. tumefuciens harbouring the rolA+rolB+rolC genes; the rooting response at 10 PM IBA was much improved. It was concluded that the 35SrolB gene and especially a combination of rolA, B and C genes promote the rooting response.Abbreviations: BAP = 6-benzylaminopurine; FeEDDHA = ferric ethylenediamine di(o-hydroxyphenylacetate); IBA = indole-3-butyric acid; LSD = least significant difference; RIM = root induction medium; SE = standard error; X-glut = 5-bromo-4-chloro-3-indolyl glucuronide

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Section: Discussionsupporting

confidence: 78%

The effects of exogenous auxin and rol genes on root formation in Rosa hybrida L. ‘Moneyway’

Salm¹,

Toorn²,

Cate³

et al. 1996

Plant Growth Regul

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“…A 24-h pulse with auxin had a maximum effect on rooting of rose (De Klerk and ter Brugge, 1992), whereas Van der Krieken et al (1993) showed that incubation of Malus stcm explants for 3 days on a medium with IBA resulted in a better root formation as compared with 2 or 5 days.…”

Section: Discussionmentioning

confidence: 93%

Biology of Root Formation and Development

Altman¹,

Waisel²

1997

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“…The observation that, in the infected cuttings treated with exogenous IBA, the meristemoids are distributed along an extensive part of the stem supports the hypothesis that IBA treatment increases the population of cells with the endogenous auxin content necessary for rooting. In apple, stem segments close to the apical bud of the cutting have a lower rooting capacity than the basal segments ; however, increasing exogenous IBA from 0.1 to 1 µM also increases the rooting response in the most apical segments (De Klerk & Ter Brugge, 1992).…”

Section: Localized Infection Positively Interacts With Iba In Enhancimentioning

confidence: 99%

How Agrobacterium rhizogenes triggers de novo root formation in a recalcitrant woody plant: an integrated histological, ultrastructural and molecular analysis

et al. 2000

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Adventitious rooting might be induced in recalcitrant woody genotypes by infection with Agrobacterium rhizogenes, and, in some cases, might also require exogenous auxin. The objective of the present study was to determine how agrobacteria trigger root formation in the stem of a recalcitrant woody microcutting, which cytological events result from the combined presence of infection and exogenous auxin, and which types of roots are induced by infection. Microcuttings of a recalcitrant walnut (Fuglans regia), infected or not with A. rhizogenes strain 1855, were cultured with either indolebutyric acid (IBA), IAA, or without exogenous hormones, to induce rhizogenesis. They were cytohistologically and ultrastructurally investigated at various times in culture. Southern blot and PCR analyses were performed to verify the frequency of transgenic, chimeric and bacterium-containing roots. The infection was sufficient per se to stimulate rhizogenesis. Rooting on the infected cuttings was enhanced by exogenous IBA, which accelerated and increased root meristemoid formation, in comparison with without hormone treatment. Meristemoids were organized both directly by the cambial cells and indirectly by the callus, and showed a pluricellular origin. Inter and intracellular bacteria were observed in the stem throughout the culture period (30 d). They were preferentially present in the vessels, and mainly in those showing polyphenol deposition. In the infected IAA-treated cultures, a high level of secondary xylem formation occurred instead of rhizogenesis. Nontransformed roots were preferentially produced by the infected cuttings treated with the auxins. Bacterium-containing and chimeric roots were produced by infected cuttings independently of the treatment. Thus, in a recalcitrant walnut, nontransformed root meristemoids are stimulated by combining infection and exogenous indolebutyric acid. Furthermore, the persistence of bacteria in the stem during the culture and the pluricellular origin of the meristemoids explain the presence of the bacterium-containing and chimeric roots

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Factors affecting adventitious root formation in microcuttings of Malus

Cited by 20 publications

References 25 publications

The effects of exogenous auxin and rol genes on root formation in Rosa hybrida L. ‘Moneyway’

The effects of exogenous auxin and rol genes on root formation in Rosa hybrida L. ‘Moneyway’

Biology of Root Formation and Development

How Agrobacterium rhizogenes triggers de novo root formation in a recalcitrant woody plant: an integrated histological, ultrastructural and molecular analysis

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