Factorial screening of antibody purification processes using three chromatography steps without protein A

Recovery and purification process development for monoclonal antibody production

“…Salt-independent antibody binding and successful elution at a somewhat higher pH range than is possible with Protein A chromatography has been demonstrated; 56,66,67 however, one critical drawback of HCIC is that it has stronger non-specific binding and can be less efficient than Protein A chromatography in reducing impurities such as host cell protein. Consequently, use of HCIC in an antibody purification process can be challenging.…”

“…Generally, a linear salt or pH gradient elution program can be conducted to determine the best elution condition. [55][56][57][58] An elution condition development study conducted for an IgG1 is shown in Figure 6. Four runs were performed in this study with two Fractogel cation exchange resins, Fractogel COO -(weak cation exchanger) chromatographic step such as cation exchange, so it may not be applicable to all products.…”

Cation-exchange chromatography of monoclonal antibodies: Characterisation of a novel stationary phase designed for production-scale purification

“…9,10 Successful use of appropriate cation exchange columns has been reported to resolve downstream bottlenecks, sustain cost-effective production, and manage large quantities. [11][12][13][14] Cation exchangers have proven to give high step yields and reduce the level of host cell proteins (hcp) very effectively. Another advantage of ion exchangers is their considerably higher chemical stability, which minimizes ligand leaching.…”

Monoclonal antibody purification with hydroxyapatite

“…Intermediate and polishing purification HA has been used for intermediate purification of many IgG [14,15,[19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36], IgA [37][38][39][40][41], and IgM antibodies [17,37,38,[42][43][44]. The majority of these applications employ simple phosphate gradients.…”