Factorial design analysis and optimisation of chitosan‐based nanogels as controlled release system for gentamicin

Abstract

The aim of this study was preparation and optimisation of a controlled-release delivery system to decrease the dosedependent side effects of gentamicin. Hydrogel nanoparticles composed of a polycationic polymer (chitosan) and an inorganic polyanion (sodium tripolyphosphate) were fabricated in the presence of gentamicin. An experimental design was drawn upon to determine the optimum condition of nanoparticle preparation. Various features of the nanoparticles including drug loading parameters, particle size distribution, zeta potential and in vitro drug release profile were evaluated. Ultimately, the antimicrobial activity of the gentamicin-loaded nanoparticles was analysed by determination of the minimum inhibitory concentration (MIC) and the potency test. As a result, the nanocarriers with an average size of about 250 nm (unloaded) and 493 nm (gentamicinloaded) were obtained with unimodal distribution and a notable polydispersity index (≤0.3). The drug loading efficiency was between 28 and 32%. The gradual and sustained releases (∼90%) of gentamicin were achieved in 24 h. The MIC and potency test showed no significant decrease in the antibacterial activity of gentamicin-loaded nanoparticles. The outcomes demonstrated that the optimised chitosan nanogels prepared in this study can be considered as a suitable carrier for a controlled release system.

D-Optimal Mixture Design: Optimization, Formulation, and Evaluation of Biosynthesis Nanogold Gels

“…Table 4 presents the Results of ANOVA for particle size. As can be seen from table 4, the model is highly statistically significant (P<0.05); also, the overall model F-value of 46.42 and P-values<0.05 show that the model is considerable model terms [22]. The p-value of the quadratic model is 0.00006 (<0.05), which means that the results are significant.…”

Linum usitatissimum Delivery over Chitosan Nanobiopolymer: Enhanced Effects on Polycystic Ovary Syndrome Condition

“…Chitosan is a natural heteropolymer, which has sparked a great interest in miscellaneous areas of medicine, especially drug delivery studies, on the basis of being biodegradable, biocompatible, soluble, and safe [ 1 ]. Hence, a large number of studies are devoted to developing advanced therapeutic drug delivery systems using this macromolecule, which is nontoxic and has a high potential for absorption-enhancing effects [ 2 , 3 ]. However, attempts are being made to address the inadequate mechanical quality of chitosan under wet conditions, as it restricts chitosan application for carrying therapeutic compounds in the body [ 4 ].…”