Factor XIII Cross-Linked Hyaluronan Hydrogels for Cartilage Tissue Engineering

Broguière, Nicolas; Cavalli, Emma; Salzmann, Gian M.; Applegate, Lee Ann; Zenobi‐Wong, Marcy

doi:10.1021/acsbiomaterials.6b00378

Cited by 61 publications

(80 citation statements)

References 46 publications

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“…ι-carrageenan is a highly sulfated polysaccharide that forms ionic crosslinks upon the addition of monovalent as well as divalent cations ( 24 ). Hyaluronan hydrogels were either crosslinked enzymatically by activated factor XIII ( 25 ) or chemically by divinyl sulfone ( 26 ). Finally, collagen, a material prevalent in tissue engineering because of its abundance in the extracellular matrix of many tissues was crosslinked with carbodiimide chemistry ( 27 ).…”

Section: Resultsmentioning

confidence: 99%

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3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Kessel¹,

Lee²,

Bonato³

et al. 2019

Preprint

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31Hydrogels are an excellent biomimetic of the extracellular matrix and have found great 32 use in tissue engineering. Nanoporous monolithic hydrogels have limited mass transport, 33 restricting diffusion of key biomolecules. Structured microbead-hydrogels overcome some 34 of these limitations, but suffer from lack of controlled anisotropy. Here we introduce a 35 novel method for producing architected hydrogels based on entanglement of microstrands. 36 The microstrands are mouldable and form a porous structure which is stable in water. 37 Entangled microstrands are useable as bioinks for 3D bioprinting, where they align during 38 the extrusion process. Cells co-printed with the microstrands show excellent viability and 39 augmented matrix deposition resulting in a modulus increase from 2.7 kPa to 780.2 kPa 40 after 6 weeks of culture. Entangled microstands are a new class of bioinks with 41 unprecedented advantages in terms of scalability, material versatility, mass transport, 42 showing foremost outstanding properties as a bioink for 3D printed tissue grafts. 43 44 45 46 47 48 49 50 129 130 131 Results 132 133 Entangled Microstrand Materials are Mouldable, Stable in Water and Macroporous 134 135Here we report on a robust and versatile method for preparing 'entangled' microstrands. 136 Bulk hyaluronan-methacrylate (HA-MA) hydrogels were mechanically pressed through a 137

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Section: Resultsmentioning

confidence: 99%

“…For HA-TG hydrogel precursors, two different batches of HA were substituted with reactive glutamine (HA-TG/Gln) and lysine (HA-TG/Lys) residues respectively following published protocols ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Kessel¹,

Lee²,

Bonato³

et al. 2019

Preprint

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“…The aim of this study was to evaluate and compare the chondrogenic potential of a recently developed tissue engineered scaffold for cartilage repair applications in four different mouse strains, namely NSG, nude, NSG-SGM3 humanized and C57BL/6. The biomaterial used was a hyaluronan-based hydrogel [22,23], produced by functionalizing the hyaluronan (HA) backbone with transglutaminase (TG) crosslinkable peptides, hereby addressed as HA-TG. The material was investigated alone and in combination with one of two cell types of different origins: human auricular chondrocytes (hAUR) and…”

Section: Introductionmentioning

confidence: 99%

A comparative study of cartilage engineered constructs in immunocompromised, humanized and immunocompetent mice

Cavalli

Fisch

Formica

et al. 2018

Journal of Immunology and Regenerative Medicine

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Highlights  Chondrogenesis of a tissue-engineered cartilage graft is feasible in immunocompetent small animals  Immunocompetent and immunodeficient animals lead to analogous results in terms of chondrogenesis, as long as the implanted cells are shielded from the host by a biomaterial  Subcutaneous implantation in small animals with a complete and human immune system could help to predict the outcome of engineered grafts for cartilage applications

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“…Firstly, HA was modified with thiol moieties (HA-SH) following, in part, a literature procedure. 19,20 As a result, HA-SH ( Fig. 1A) was obtained in adequate yield (82% ± 8.7%) with a high degree of modification (39.7% ± 1.8% of the disaccharide units were functionalized with the thiol group, as determined by 1 H NMR spectroscopy, Fig.…”

mentioning

confidence: 92%

“…16 In contrast, HA often requires chemical modifications to introduce functional groups that are then able to cross-link the HAbased network (e.g. methacrylates, 17 thiols, 18 enzymes, 19,20 amino acids (lysine 21 or tyramine 22 ), gallol conjugates, 23 and Schiff's bases, 24,25 among others 26,27 ). In addition, supramolecular assembly of guest-host pairs produces physically-crosslinked HA hydrogels.…”

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confidence: 99%