Factor VIII related antigen as an endothelial cell marker in benign and malignant diseases

Abstract

The presence and distribution of Factor VIII related antigen (FVIIR:Ag) in formation fixed, paraffin embedded tissue were studied in benign and malignant vascular tumors, inflammatory vascular diseases, normal tissue from various organs and a number of malignant tumors. The unlabeled peroxidase-anti-peroxidase method was utilized. Immunostaining was observed only in endothelial cells, in tumor cells of endothelial cell origin and in megakaryocytes and platelets. The staining method gave a distinct picture of the vascular pattern in all types of tissue examined. The demonstration of FVIIIR:Ag by means of the immunoperoxidase technique is considered a valuable method in diagnosing tumors of vascular origin. The method also facilitates detection of vascular invasion of malignant tumors in small caliber vessels.

Gap Junction Localization and Connexin Expression in Cytochemically Identified Endothelial Cells of Arterial Tissue

“…Furthermore, because two of the connexins reported to be expressed in endothelial cells, connexin43 and connexin40, have also been reported in vascular smooth muscle cells (Beyer et al 1992;Pepper et al 1992;Blackburn et al 1995;Little et al 1995a;Burt 1994,1995), the two cell types cannot be reliably distinguished on the basis of connexin immunolabeling patterns alone. All the interpretative uncertainties arising from these sources are overcome by our dual cell marking/connexin labeling technique, in which use of either a specific lectin or labeling of the characteristic endothelial product VWF (Wharton et al 1990;Sehested and Hou-Jensen 1981), permits unequivocal correlation of connexin labeling with the endothelial layer.…”

Anti-transforming growth factor (TGF)-beta antibodies inhibit breast cancer cell tumorigenicity and increase mouse spleen natural killer cell activity. Implications for a possible role of tumor cell/host TGF-beta interactions in human breast cancer progression.

“…1% CaC12. This was followed by a peroxidase-antiperoxidase (PAP) histochemical procedure using a 1:200 dilution ofthe antihuman von Willebrand factor purified Ig fraction of rabbit antiserum (Dakopatts, Glostrup, Denmark) as previously described (22). Nonimmune rabbit Ig fraction was used as control.…”

Single-laser three-color immunolabeling of a histological section by laser scanning microscopy: application to senile plaque-related structures in post-mortem human brain tissue.

“…Before staining, the sections were treated in formic acid (>99%) for 1 min. After pre-incubation with the blocking serum (596, goat) in PBS-Tx, the sections were incubated for 2 days with the first primary antibody, anti-vWF (1:500, polyclonal IgG raised in rabbit) (Dako; Glostrup, Denmark (8) diluted with PBS-E containing 5 % goat serum. The sections were then incubated for 6 hr with a goat anti-rabbit IgG conjugated with F I E (1:200) (Cappel; Durham, NC) diluted with PBS-E containing 5% goat serum, followed by incuba-tion with 50% normal rabbit serum in PBS-E for 1 hr to block the unoccupied binding sites of the FITC-conjugated anti-rabbit IgG.…”

“…These fluorochromes are excited by a single 488-nm laser beam. HLA-DR and von Willebrand factor ( v m ) are used as specific markers for microglia (6,7) and vascular endothelial cells (8), respectively. With this method, the spatial relationship among blood vessels, reactive microglia, and amyloid P-protein (AB) deposits in the brain of patients with Alzheimer's disease (AD) is clearly visualized in a single photomicrograph.…”