Factor V Gene Polymorphism (G1691A) Among Sudanese Patients with Intracerebral Hemorrhage, Khartoum State, 2022

Abstract

Background Intracranial hemorrhage refers to any bleeding within the intracranial vault, including the brain parenchyma and surrounding meningeal spaces. In Sudan, there is no published data regarding factor V Leiden mutation. Therefore this study was designed to detect the possible present of factor V polymorphism (G1691A) among Sudanese patients with intercereberal hemorrhage. Material and method This study was a cross sectional hospital-based study, conducted at the research laboratory of the national center of neurological sciences (NCNS), Khartoum, Sudan during the period June 2022 to August 2022.It included all patients attended with Intracerebral hemorrhage, DNA extraction was done from blood of all patients and controls.PCR for factor V gene was done and thus Sanger sequencing. Results The PCR results showed; 100% were positive for factor V gene. Sequencing results revealed single based exchange in factor V geneG to A (G1691A). Conclusion Factor V gene polymorphism (G1691A) was detected and might be in association with intercereberal hemorrhage among Sudanese patients