The Edinburgh Handedness Inventory (EHI) is persistently the most used inventory to evaluate handedness, being neuropsychological investigation and clinical practice. Despite this, there is no information on how this instrument functions in a Portuguese population. The objective of this study was therefore to examine the sociodemographic influences on handedness and establish psychometric properties of the EHI in a Portuguese sample. The sample consisted of 342 adults (157 men and 185 women), assessed with a battery of neuropsychological tests. The mean EHI Laterality Quotient was 63.52 (SD ¼ 38.00). A much high percentage of ambiguous-handedness compared to left-handedness was detected. An inconsistency was found between the preference for formal education activities (writing-drawing-using scissors) and the remaining EHI activities. From sociodemographic variables, only age, area, and regions of residence showed significant influence on EHI scores. The reliability and temporal reliability of EHI were adequate. Confirmatory factor analysis indicated a one-factor model (� 2 /df ¼ 2.141; TLI ¼ 0.972; CFI ¼ 0.979; RMSEA ¼ 0.058). The inconsistency between formal education and nonformal activities could be an indicator of social pressure. The present data give support for the notion that handedness measured by EHI is potentially sensitive to sociodemographic and cultural influences. KEYWORDS Edinburgh handedness; hand preference; handedness; inventory; psychometric properties 65 handedness) (Dragovic, Milenkovic, & Hammond, 2008). To evaluate handedness, the two methods most commonly used include observation of the use of the dominant hand and the application of inventories answered by the individual (Barbieri & Gobbi, 2009). 70 The three most popular inventories (van Strien, 2002) are by Crovitz and Zener (1962 Q5), Annett (1970), and Oldfield (1971). The Edinburgh Handedness Inventory (EHI; Oldfield, 1971) is the most used from the previous three (Fazio & Cantor, 2015; Veale, 2014). The EHI 75 offers the advantage of being a simple and brief method of evaluating laterality using a quantitative scale (Oldfield, 1971). The first version of the Oldfield Inventory was based on a modified version of the Humphrey inventory with 80 20 items (Büsch, Hagemann, & Bender, 2010). There is