FACS analysis of bovine leukemia virus (BLV)-infected cell lines with monoclonal antibodies (mAbs) to B cells and to monocytes/ macrophages

Letesson, Jean‐Jacques; Broecke, A. van den; Marbaix-Cleuter, Y; Delcommenne, Marc; Mager, A.; Mammerickx, Marc; Burny, Arsène; Depelchin, A.

doi:10.1016/0165-2427(91)90102-i

Cited by 17 publications

(7 citation statements)

References 12 publications

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“…They have been also found in various B cell tumors of mice and B cell chronic lymphocyte leukemia in humans [7,11,14]. The same cells have also been observed in normal B lymphocytes and tumor-like B cells infected with BLV [8,13,20,23].…”

mentioning

confidence: 63%

Relation between Phenotype of Tumor Cells and Clinicopathology in Bovine Leukosis

Yin

Makara

Pan

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Thirty-three cases of enzootic bovine leukosis (EBL) and 14 cases of sporadic bovine leukosis (SBL) were examined by immunohistochemistry using 6 monoclonal antibodies against leukocyte differentiation molecules of bovine leukocytes. There were 17 cases of B-1a cell type, 10 cases of B-1b cell type and 6 cases of B-2 cell type in EBL, and 5 cases originating from B cells (B-2 cell type) and 9 cases originating from immature T cells in SBL. The average age for the EBL cases of B-1a cell type was 8.6 years, B-1b cell type was 6.5 years, and of B-2 cell type was 4.5 years. In cases of SBL, immature T cell type patients were younger than B-2 ce ll type ones. The lymphoma originating from B cells differed from that originating from T cells in morphology. In T cell tumors, the nucleus of tumor cells was round, the edge of the cytoplasm obvious, and tumor cells were sporadically present and proliferated. When compared with T cells, the region among B cells was obscure. But, there was no relation between phenotype and the histologic classification of tumor cells. In EBL, beyond the lymph node, tumors of B-1a and B-1b types had developed in the heart and abomasum, and those of the B-2 type tended to occur in liver. In SBL, B-2 type and T type cells formed tumors in the liver, kidney, thymus, and one case of T-cell type tumor formed on the skin. We would like to propose a new classification of bovine leukosis as EBL, calf type B-cell lymphoma, juvenile T-cell lymphoma and skin type T-cell lymphoma.

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confidence: 63%

Relation between Phenotype of Tumor Cells and Clinicopathology in Bovine Leukosis

Yin

Makara

Pan

et al. 2003

The Journal of Veterinary Medical Science

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“…Persistent lymphocytosis in BLV-infected sheep and cattle is characterized by an expansion of the B-cell population coexpressing CD5 and myeloid markers (7,30,31). CD5+ B cells in cattle and humans are considered to be the equivalent of Lyl + murine B cells and express the usual B-cell markers as well as myeloid markers and an IgMhigh IgDIow phenotype (14,38).…”

Section: Discussionmentioning

confidence: 99%

In vivo leukocyte tropism of bovine leukemia virus in sheep and cattle

Schwartz

Bensaïd

Polack

et al. 1994

J Virol

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Bovine leukemia virus (BLV), an oncovirus related to human T-cell leukemia virus type I, causes a B-cell lymphoproliferative syndrome in cattle, leading to an inversion of the T-cell/B-cell ratio and, more rarely, to a B-cell lymphosarcoma. Sheep are highly sensitive to BLV experimental infection and develop B-cell pathologies similar to those in cattle in 90%o of the cases. BLV tropism for B cells has been well documented, but the infection of other cell populations may also be involved in the BLV-induced lymphoproliferative syndrome. We thus looked for BLV provirus in other leukocyte populations in sheep and cattle by using PCR. We found that while B cells harbor the highest proviral load, CD8+ T cells, monocytes, and granulocytes, but not CD4+ T cells, also bear BLV provirus. As previously described, we found that persistent lymphocytosis in cows is characterized by an expansion of the CD5+ B-cell subpopulation but we did not confirm this observation in sheep in which the expanded B-cell population expressed the CD11b marker. Nevertheless, BLV could be detected both in bovine CD5+ and CD5-B cells and in sheep CD11b+ and CDllb-B cells, indicating that the restricted BLV tropism for a specific B-cell subpopulation cannot explain its expansion encountered in BLV infection. Altogether, this work shows that BLV tropism in leukocytes is wider than previously thought. These results lead the way to further studies of cellular interactions among B cells and other leukocytes that may intervene in the development of the lymphoproliferative syndrome induced by BLV infection.

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“…This conclusion holds true if the B lymphocyte population is considered as homogeneous, and if all putative subpopulations of cells replicate at similar rates. Of note, phenotypic characterization by means of CD5 or CD11b markers, two molecules suggested to be associated with BLV infection (33,45), did not reveal any preferential proliferation of a given B cell subtype (data not shown). However, long-term analysis of BrdUrd incorporation demonstrated that a significant proportion of B lymphocytes might remain positive even at 44 days after labeling (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…After 18 h of culture, PBMCs were collected, labeled with 1H4 antibody recognizing surface immunoglobulin M (sIgM; ref. 33) and with a fluorescein isothiocyanate (FITC)-conjugate (Dako). The cells were stained with propidium iodide (PI; Sigma) and analyzed by flow cytometry, as described (17).…”

Section: Isolation Of Pbmcs and Analysis Of In Vitro (Ex Vivo) Apoptomentioning

confidence: 99%

Increased cell proliferation, but not reduced cell death, induces lymphocytosis in bovine leukemia virus-infected sheep

Debacq

Asquith

Kerkhofs

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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Lymphocyte homeostasis is the result of a critical balance between cell proliferation and death. Disruption of this subtle equilibrium can lead to the onset of leukemia, an increase in the number of lymphocytes being potentially due to both of these parameters. The relative importance of cell proliferation vs. apoptosis during pathogenesis induced by the primate T cell lymphotropic viruses and bovine leukemia virus (BLV) has been difficult to assess because of conflicting data from a range of in vitro and ex vivo experimental systems. Here, we aim to resolve this issue by measuring the rates of cell proliferation and death in the BLV-ovine system, an animal model of human T lymphotropic virus (HTLV-1). We use a method based on the i.v. injection of 5-bromodeoxyuridine into BLV-infected sheep. We show that B lymphocytes in BLV ؉ asymptomatic sheep proliferate significantly faster than in uninfected controls (average proliferation rate: 0.020 per day vs. 0.011 per day). In contrast, the rates of cell death were not significantly different between aleukemic BLV-infected and control sheep (average death rate 0.089 per day vs. 0.094 per day, respectively). We conclude that the increase in the number of B cells during BLVinduced lymphocytosis results from higher proliferation rates but is not due to a significant decrease in apoptosis, in contrast to data from in vitro (ex vivo) experiments. The imbalance created by the net increase in proliferation in the absence of compensating cell death reveals a complex mechanism of feedback regulation controlling homeostasis in the blood compartment.

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FACS analysis of bovine leukemia virus (BLV)-infected cell lines with monoclonal antibodies (mAbs) to B cells and to monocytes/ macrophages

Cited by 17 publications

References 12 publications

Relation between Phenotype of Tumor Cells and Clinicopathology in Bovine Leukosis

Relation between Phenotype of Tumor Cells and Clinicopathology in Bovine Leukosis

In vivo leukocyte tropism of bovine leukemia virus in sheep and cattle

Increased cell proliferation, but not reduced cell death, induces lymphocytosis in bovine leukemia virus-infected sheep

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