Facklamia Species as an Underrecognized Pathogen

Rahmati, Elham; Martín, Vanessa; Wong, Darren; Sattler, Fred R.; Petterson, Jonas; Ward, Pamela; Butler-Wu, Susan M.; She, Rosemary C.

doi:10.1093/ofid/ofw272

Cited by 36 publications

(34 citation statements)

References 13 publications

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“…Enterorhabdus has been implicated in the development of obesity (Wang et al 2016), autism spectrum disorders (de Theije et al 2014), and is predominant in prediabetic patients (Yang et al 2015). Facklamia, alpha-haemolytic bacteria, have been shown to cause bacteraemia (Rahmati et al 2017), acute cystitis and sepsis (Mostafa et al 2019). Veillonella species have been reported to be aetiological factor of endocarditis (Saladi et al 2017) and associated with Crohn's disease (Kugathasan et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Prevention of excessive exercise‐induced adverse effects in rats withBacillus subtilisBSB3

et al. 2019

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Aims To characterize efficacy of the Bacillus subtilis BSB3 (BSB3) strain in the prevention of excessive exercise‐induced side effects and in maintaining stability of the gut microbiota. Methods and Results Rats were pretreated by oral gavage with B. subtilis BSB3 (BSB3) or with phosphate‐buffered saline (PBS) twice a day for 2 days, and were either exposed forced treadmill running or remained sedentary. Histological analysis of intestine, immunofluorescence staining of tight junction (TJ) proteins, serum lipopolysaccharide and intestinal fatty acid‐binding protein assay, culture‐based analysis and pyrosequencing for the gut microbiota were performed for each rat. Forced running resulted in a substantial decrease in intestinal villi height and total mucosa thickness, the depletion of Paneth cells, an inhibition of TJ proteins expression. Short‐term treatment of rats with BSB3 before running prevented these adverse effects. Culture‐based analysis of the gut microbiota revealed significant elevation of pathogenic microorganisms only in treadmill‐exercised rats pretreated with PBS. High‐throughput 16S rRNA gene sequencing also revealed an increase in pathobionts in this group. Preventive treatment of animals with BSB3 resulted in predominance of beneficial bacteria. Conclusions BSB3 prevents excessive exercise‐associated complications by beneficial modulation of the gut microbiota. Significance and Impact of the Study Our study shows a new application of beneficial bacteria for prevention the adverse effects of excessive exercise.

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Section: Discussionmentioning

confidence: 99%

Prevention of excessive exercise‐induced adverse effects in rats withBacillus subtilisBSB3

et al. 2019

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“…The rst detection of Facklamia languida extends the number of BV-associated bacteria reported in the literature up to now [49][50][51][52][53]. Interestingly, Facklamia species have recently been associated with the etiology of numerous clinical infections [54]. The microbial population detected in bacterial vaginosis ora was greatly similar to bacterial communities found in human faeces, with the presence of bacteria belonging to the genera Prevotella, Peptoniphilus, and Clostridium [55].…”

Section: Discussionmentioning

confidence: 58%

Characterisation of the Vaginal Microbiota Using Culturomics and Metagenomics Suggests Transplantation of Gut Microbiota Into the Vagina During Bacterial Vaginosis

Diop

Fall

Levasseur

et al. 2020

Preprint

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Background: Bacterial vaginosis is a very common vaginal disorder, with a gynaeco-obstetrical impact, but remains poorly understood. Current antibiotic treatment most often fails. Our objective was to exhaustively map the bacterial community present in bacterial vaginosis and normal flora, using not only a molecular approach but also a highly efficient culture approach, culturomics. Vaginal bacterial diversity was evaluated using both strategies for 24 Caucasian French women, including 7 cases of bacterial vaginosis, and 10 rural Senegalese women, including 5 cases of bacterial vaginosis. An additional 16 specimens (three cases of bacterial vaginosis and 13 normal flora) obtained during the follow-up visits of five French women with bacterial vaginosis were analysed using metagenomics.Results: The combination of culturomics and metagenomics reveals the richness and diversity of vaginal microbiota. Our data demonstrated that compared to healthy women, those with bacterial vaginosis present a shift in vaginal flora. The microbiota of the bacterial vaginosis group can be easily distinguished from that of healthy group by their increased bacterial diversity, abundance of Bacteroidales and Leptotrichiaeceae, and depletion of Proteobacteria and Lactobacillaceae species. In addition, a complex of 10 genera was associated with bacterial vaginosis: Gardnerella, Atopobium, Snethia, Aerococcus, Prevotella, Gemella, Facklamia, Porphyromonas, Mycoplasma, and Urinacoccus. Overall, the microbial population detected in bacterial vaginosis flora was largely similar to the bacterial communities found in gut microbiota. The two approaches showed a diverse vaginal microbiota that was largely non-overlapping, with only 146 common species. Furthermore, culturomics extends the repertoire of human-associated bacteria with the isolation of 27 new bacterial species.Conclusions: This study provides the most representative topology of the vaginal microbiota of normal flora and bacterial vaginosis and suggests the transplantation of gut microbiota into the vagina during bacterial vaginosis. Several sets of clinically relevant anaerobic bacteria and new species were also isolated from the human vagina, confirming that some previously “uncultivated” species may be cultivated using an appropriate strategy. Exhaustive characterisation of vaginal microbiota will enable a better understanding and management of bacterial vaginosis. Obtaining isolates will allow us to explore the bacterial interactions of the vaginal microbiota and will serve as a basis for the potential development of bacteriotherapy to prevent or treat vaginal dysbiosis.

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“…and Micrococcus spp. are considered to be part of the normal flora of the female genital tract . Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Limitations of a chromogenic agar plate for the identifying bacteria isolated from equine endometritis samples

Vera

Boyen

Visscher

et al. 2018

Equine Veterinary Journal

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Facklamia Species as an Underrecognized Pathogen

Cited by 36 publications

References 13 publications

Prevention of excessive exercise‐induced adverse effects in rats withBacillus subtilisBSB3

Prevention of excessive exercise‐induced adverse effects in rats withBacillus subtilisBSB3

Characterisation of the Vaginal Microbiota Using Culturomics and Metagenomics Suggests Transplantation of Gut Microbiota Into the Vagina During Bacterial Vaginosis

Limitations of a chromogenic agar plate for the identifying bacteria isolated from equine endometritis samples

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