Facilities For Animal Research In Space

Bonting, S.L.; Kishiyama, Jenny S.; Arno, R. D.

doi:10.1016/s1569-2574(08)60127-6

Cited by 6 publications

(1 citation statement)

References 6 publications

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“…Both Immune 1 and 2 were 8-day flights that lasted 193 and 198 h, respectively. Animals were housed in NASA's animal enclosure module (AEM) (8,46), with six rats per AEM, two AEMs per flight, in the shuttle middeck. During Immune 1, animals were exposed to a mean temperature of 25.0 Ϯ 0.1(SE)°C (range, 21.9-26.3°C) and an average of 26.3 Ϯ 0.1°C (range, 24.7-29.4°C) during Immune 2.…”

Section: Methodsmentioning

confidence: 99%

Effects of spaceflight and PEG-IL-2 on rat physiological and immunological responses

Chapes

Simske

Sonnenfeld

et al. 1999

Journal of Applied Physiology

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Sprague-Dawley rats were subjected to two 8-day spaceflights on the space shuttle. Rats housed in the National Aeronautics and Space Administration's animal enclosure were injected (iv or sc) with pegylated interleukin-2 (PEG-IL-2) or a placebo. We tested the hypothesis that PEG-IL-2 would ameliorate some of the effects of spaceflight. We measured body and organ weights; blood cell differentials; plasma corticosterone; colony-forming units (macrophage and granulocyte macrophage); lymphocyte mitogenic, superantigenic, and interferon-gamma responses; bone marrow cell and peritoneal macrophage cytokine secretion; and bone strength and mass. Few immunological parameters were affected by spaceflight. However, some spaceflight effects were observed in each flight. Specifically, peritoneal macrophage spontaneous secretion of tumor necrosis factor-alpha occurred in the first but not in the second flight. A significant monocytopenia and lymphocytopenia were detected in the second but not in the first flight. The second mission produced bone changes more consistent with past spaceflight investigations. PEG-IL-2 did not appear to be beneficial; however, this was mostly due to the lack of spaceflight effects. These studies reflect the difficulty in reproducing experimental models by using current space shuttle conditions.

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Section: Methodsmentioning

confidence: 99%

Effects of spaceflight and PEG-IL-2 on rat physiological and immunological responses

Chapes

Simske

Sonnenfeld

et al. 1999

Journal of Applied Physiology

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Effects of spaceflight on rat peripheral blood leukocytes and bone marrow progenitor cells

Ichiki

Gibson

Jago

et al. 1996

Journal of Leukocyte Biology

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The white blood cell (WBC) elements and the bone marrow myeloid progenitor cell populations were analyzed to ascertain adaptation to micro-gravity and subsequent readaptation to 1 G in rats flown on the 14-day Spacelab Life Sciences-2 (SLS-2) mission. Bone marrow cells were harvested from one group of rats killed inflight (FD13) and blood was drawn from three other groups at various times. The WBC level was normal on FD14 with the exception of neutrophilia. On FD13, numbers of colony-forming units-granulocyte (CFU-G), CFU-GM, and CFU-M from flight animals were decreased compared with ground controls when incubated with recombinant rat interleukin-3 (rrIL-3) alone or in combination with recombinant human erythropoietin (rhEpo). On recovery (R + 0), flight rats had decreased numbers of total leukocytes and absolute numbers of lymphocytes and monocytes with elevated neutrophils compared with control rats. They had lower numbers of CD4, CD8, CD2, CD3, and B cells in the peripheral blood but no differences in spleen lymphocytes.

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Medical and surgical applications of space biosensor technology

Hines

1996

Acta Astronautica

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Facilities For Animal Research In Space

Cited by 6 publications

References 6 publications

Effects of spaceflight and PEG-IL-2 on rat physiological and immunological responses

Effects of spaceflight and PEG-IL-2 on rat physiological and immunological responses

Effects of spaceflight on rat peripheral blood leukocytes and bone marrow progenitor cells

Medical and surgical applications of space biosensor technology

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