Facilitating THP-1 macrophage studies by differentiating and investigating cell functions in polystyrene test tubes

Small, Annabelle; Lansdown, Nikki; Al-Baghdadi, Marwah; Quach, Alex; Fèrrante, Antonio

doi:10.1016/j.jim.2018.06.019

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…60 To perform this experiment, human THP-1 monocytes were first differentiated into macrophages using phorbol 12-myristate 13-acetate (PMA) (Figure S4). 61,62 We then treated the macrophages with bare and active protein-coated SNAs for 1−8 h at 37 °C. We hypothesized that IgG preadsorption would improve the SNA uptake efficiency by macrophages while HSA preadsorption would reduce it.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…To perform this experiment, human THP-1 monocytes were first differentiated into macrophages using phorbol 12-myristate 13-acetate (PMA) (Figure S4). , We then treated the macrophages with bare and active protein-coated SNAs for 1–8 h at 37 °C. We hypothesized that IgG preadsorption would improve the SNA uptake efficiency by macrophages while HSA preadsorption would reduce it. , Interestingly, both IgG and HSA adsorption lowered the uptake of the SNAs into the macrophages, with HSA adsorption having a more significant impact on reducing SNA clearance by macrophages (Figure B).…”

Section: Resultsmentioning

confidence: 99%

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Spherical Nucleic Acids with Tailored and Active Protein Coronae

2019

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Spherical nucleic acids (SNAs) are nanomaterials typically consisting of a nanoparticle core and a functional, dense, and highly oriented oligonucleotide shell with unusual biological properties that make them appealing for many applications, including sequence-specific gene silencing, mRNA quantification, and immunostimulation. When placed in biological fluids, SNAs readily interact with serum proteins, leading to the formation of ill-defined protein coronae on the surface, which can influence the targeting capabilities of the conjugate. In this work, SNAs were designed and synthesized with functional proteins, such as antibodies and serum albumin, deliberately adsorbed onto their surfaces. These particles exhibit increased resistance to protease degradation compared with native SNAs but still remain functional, as they can engage in hybridization with complementary oligonucleotides. SNAs with adsorbed targeting antibodies exhibit improved cellular selectivity within mixed cell populations. Similarly, SNAs coated with the dysopsonizing protein serum albumin show reduced macrophage uptake, providing a strategy for tailoring selective SNA delivery. Importantly, the protein coronae remain stable on the SNAs in human serum, exhibiting a less than 45% loss of protein through exchange after 12 h at 37 °C. Taken together, these results show that protein–SNA complexes and the method used to prepare them provide a new avenue for enhancing SNA stability, targeting, and biodistribution.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Spherical Nucleic Acids with Tailored and Active Protein Coronae

2019

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“…The human monocyte cell line THP-1 is widely used in research thanks to the ability of monocytes to differentiate into macrophages [24]. THP-1 monocytes have a round shape in suspension; when they differentiate upon stimulation by phorbol 12-myristate-13-acetate (PMA), the cells adhere to the culture plates, gaining phenotypic and functional characteristics similar to primary human macrophages [25,26].…”

Section: Introductionmentioning

confidence: 99%

Candidate Polyurethanes Based on Castor Oil (Ricinus communis), with Polycaprolactone Diol and Chitosan Additions, for Use in Biomedical Applications

et al. 2019

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Polyurethanes are widely used in the development of medical devices due to their biocompatibility, degradability, non-toxicity and chemical versatility. Polyurethanes were obtained from polyols derived from castor oil, and isophorone diisocyanate, with the incorporation of polycaprolactone-diol (15% w/w) and chitosan (3% w/w). The objective of this research was to evaluate the effect of the type of polyol and the incorporation of polycaprolactone-diol and chitosan on the mechanical and biological properties of the polyurethanes to identify the optimal ones for applications such as wound dressings or tissue engineering. Polyurethanes were characterized by stress-strain, contact angle by sessile drop method, thermogravimetric analysis, differential scanning calorimetry, water uptake and in vitro degradation by enzymatic processes. In vitro biological properties were evaluated by a 24 h cytotoxicity test using the colorimetric assay MTT and the LIVE/DEAD kit with cell line L-929 (mouse embryonic fibroblasts). In vitro evaluation of the possible inflammatory effect of polyurethane-based materials was evaluated by means of the expression of anti-inflammatory and proinflammatory cytokines expressed in a cellular model such as THP-1 cells by means of the MILLIPLEX® MAP kit. The modification of polyols derived from castor oil increases the mechanical properties of interest for a wide range of applications. The polyurethanes evaluated did not generate a cytotoxic effect on the evaluated cell line. The assessed polyurethanes are suggested as possible candidate biomaterials for wound dressings due to their improved mechanical properties and biocompatibility.

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“…as described previously [18]. Human AB serum was prepared from peripheral blood of healthy volunteers.…”

Section: Using Commercially Available Labelled Zymosan a Bioparticlesmentioning

confidence: 99%

The Role of Phagocytes in Immunity to Candida albicans

Small¹,

King²,

Rathjen³

et al. 2019

Candida Albicans

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Body clearance of fungi such as Candida albicans involves phagocytosis by fixed tissue macrophages as well as infiltrating monocytes and neutrophils. Through phagocytosis, the fungi are confined and killed by the oxidative and non-oxidative anti-microbial systems. These include oxygen derived reactive species, generated from the activation of the NADPH oxidase complex and granule constituents. These same mechanisms are responsible for the damage to hyphal forms of C. albicans. Complement promotes phagocytosis, through their interaction with a series of complement receptors including the recently described complement receptor immunoglobulin. However, it is also evident that under other conditions, the killing of yeast and hyphal forms can occur in a complement-independent manner. Phagocytosis and killing of Candida is enhanced by the cytokine network, such as tumour necrosis factor and interferon gamma. Patients with primary immunodeficiency diseases who have phagocytic deficiencies, such as those with defects in the NADPH oxidase complex are predisposed to fungal infections, providing evidence for the critical role of phagocytes in anti-fungal immunity. Secondary immunodeficiencies can arise as a result of treatment with anticancer or other immunosuppressive drugs. These agents may also predispose patients to fungal infections due to their ability to compromise the anti-microbial activity of phagocytes.

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Facilitating THP-1 macrophage studies by differentiating and investigating cell functions in polystyrene test tubes

Cited by 5 publications

References 10 publications

Spherical Nucleic Acids with Tailored and Active Protein Coronae

Spherical Nucleic Acids with Tailored and Active Protein Coronae

Candidate Polyurethanes Based on Castor Oil (Ricinus communis), with Polycaprolactone Diol and Chitosan Additions, for Use in Biomedical Applications

The Role of Phagocytes in Immunity to Candida albicans

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