Facilitated Production of Secretory IgA against Shiga Toxin B Subunits by Intranasal Application of Antigen‐Coated Polystyrene Microspheres

Kurohane, Kohta; Kobayashi, Chie; Imai, Yumiko

doi:10.1111/j.1348-0421.2005.tb03714.x

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…It may be possible that IgG ASC traffic predominately through peripheral lymphoid tissues following immunization. IgA is a critical component of the mucosal immune response and its secretion upon mucosal immunization provides an important first line of defense against invasion of bacterial and viral pathogens [42], [43].…”

Section: Discussionmentioning

confidence: 99%

Outer Membrane Protein A (OmpA) of Shigella flexneri 2a, Induces Protective Immune Response in a Mouse Model

et al. 2011

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BackgroundIn our earlier studies 34 kDa outer membrane protein (OMP) of Shigella flexneri 2a has been identified as an efficient immunostimulant.Key ResultsIn the present study MALDI-TOF MS analysis of the purified 34 kDa OMP of Shigella flexneri 2a shows considerable sequence homology (Identity 65%) with the OmpA of S. flexneri 2a. By using the specific primers, the gene of interest has been amplified from S. flexneri 2a (N.Y-962/92) genomic DNA, cloned in pET100/D-TOPO® vector and expressed using induction with isopropyl thiogalactoside (IPTG) for the first time. Immunogenicity and protective efficacy of the recombinant OmpA has been evaluated in an intranasally immunized murine pulmonary model. The recombinant protein induces significantly enhanced protein specific IgG and IgA Abs in both mucosal and systemic compartments and IgA secreting cells in the systemic compartment (spleen). The mice immunized with OmpA have been protected completely from systemic challenge with a lethal dose of virulent S. flexneri 2a. Immunization with the protein causes mild polymorphonuclear neutrophil infiltration in the lung, without inducing the release of large amounts of proinflammatory cytokines.ConclusionThese results suggest that the OmpA of S. flexneri 2a can be an efficacious mucosal immunogen inducing protective immune responses. Our findings also demonstrate that antibodies and Th1 immune response may be associated with the marked protective efficacy of immunized mice after intranasal shigellae infection.

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Section: Discussionmentioning

confidence: 99%

Outer Membrane Protein A (OmpA) of Shigella flexneri 2a, Induces Protective Immune Response in a Mouse Model

et al. 2011

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“…Oral or nasal immunization elicits both mucosal and systemic immune responses. We have demonstrated that nasal immunization with Stx1B plus CT induced mucosal anti‐Stx1B IgA as well as serum anti‐Stx1B IgG [28, 29]. In our previous study, an Stx1B‐specific IgA mAb, G2G7, was established using B cells from nasal‐associated lymphoid tissues after intranasal immunization with cross‐linked Stx1B [22].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Monoclonal Immunoglobulin A and G Against Shiga Toxin Binding Subunits Produced by Intranasal Immunization

et al. 2008

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Immunoglobulin A (IgA) is considered to play a major role in protection of the mucosal surface. However, its immunological and biological properties have not been extensively studied because the production of IgA class monoclonal antibodies (mAbs) is difficult. We compared the properties of IgA and IgG mAbs against Shiga toxin B subunits (Stx1B). These mAbs were secreted from hybridomas that had been produced from mice after intranasal immunization with recombinant Stx1B and cholera toxin. The dose response curves for the binding of the IgA (clone G2G7) and IgG (clone D11C6) mAbs to immobilized Stx1B were similar, as revealed on ELISA. The majority of the IgA mAb formed dimers while the IgG mAb was monomeric, as judged by immunoblot analysis. The IgG mAb completely inhibited the binding of Stx1B to Burkitt’s lymphoma cell line Ramos, while the inhibition by the IgA mAb was only partial. The IgG mAb was able to neutralize the cytotoxicity of Stx1 holotoxin towards Vero cells, whereas the IgA mAb was not. The binding affinity of each binding site was compared by means of surface plasmon resonance analysis involving a capture method, with which the binding of soluble Stx1B to immobilized mAb was detected. The association rate was similar but the dissociation rate was twofold faster in the case of the IgA mAb, resulting in twofold higher affinity of the IgG mAb. These results suggest that one can obtain high affinity IgA mAb but toxin neutralization is another challenge as to therapeutic antibodies of the IgA class.

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“…In addition, the fabrication of a polymer 'cushion' on the surface of inorganic substrates such as Au or TiO 2 can improve biosensor or medical implant function by minimizing conformational changes to biomolecules, typically associated with their direct adsorption on a metal surface [3]. Other areas of applications may include e.g., nanoelectromechanical systems [4], micro-fluidic devices [5,6] and drug delivery systems [7] all of which may also benefit from controlled construction of polymeric nanopatterns on metal surfaces.…”

Section: Introductionmentioning

confidence: 99%

Tunable 3D and 2D polystyrene nanoparticle assemblies using surface wettability, low volume fraction and surfactant effects

et al. 2008

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Polymer-based nanopatterning on metal surfaces is of increasing importance to a number of applications, including biosensors, bioelectronic devices and medical implants. Here we show that polycrystalline gold surfaces can be functionalized with monocomponent nanoparticle (NP) assemblies by a simple drop deposition method. Ordered 3D hexagonal close-packed structures consisting of 350 nm polystyrene (PS) NPs on hydrophobically modified gold surfaces from solutions of very low volume fraction (varphi = 0.0006) were obtained as a result of capillary force induced self-assembly, whilst 2D self-assembly of PS NPs was generated over large area on hydrophilic gold and TiO(2) surfaces by spin coating. Furthermore, we show that when Triton X-100 is added to the PS NP suspending medium longer range ordering is obtained. Our observations may initiate interesting applications in the areas of nanoengineering of metal-based sensors and as a means to design new nanostructures for biocompatible implant surfaces.

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Facilitated Production of Secretory IgA against Shiga Toxin B Subunits by Intranasal Application of Antigen‐Coated Polystyrene Microspheres

Cited by 4 publications

References 38 publications

Outer Membrane Protein A (OmpA) of Shigella flexneri 2a, Induces Protective Immune Response in a Mouse Model

Outer Membrane Protein A (OmpA) of Shigella flexneri 2a, Induces Protective Immune Response in a Mouse Model

Characterization of Monoclonal Immunoglobulin A and G Against Shiga Toxin Binding Subunits Produced by Intranasal Immunization

Tunable 3D and 2D polystyrene nanoparticle assemblies using surface wettability, low volume fraction and surfactant effects

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